ID   NCTB_ASPFU              Reviewed;         142 AA.
AC   Q4WFF8;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   17-APR-2007, sequence version 2.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=NCT transcriptional regulatory complex subunit B {ECO:0000303|PubMed:31969561};
DE   AltName: Full=Negative cofactor 2 B {ECO:0000303|PubMed:31969561};
GN   Name=nctB {ECO:0000303|PubMed:31969561}; ORFNames=AFUA_3G02340;
OS   Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS   A1100) (Aspergillus fumigatus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=330879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX   PubMed=16372009; DOI=10.1038/nature04332;
RA   Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA   Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA   Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA   Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA   Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA   Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA   Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA   Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA   Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA   Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA   O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA   Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA   Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA   Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA   Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA   Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA   Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA   Barrell B.G., Denning D.W.;
RT   "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT   Aspergillus fumigatus.";
RL   Nature 438:1151-1156(2005).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH NCTA AND MOT1.
RX   PubMed=31969561; DOI=10.1038/s41467-019-14191-1;
RA   Furukawa T., van Rhijn N., Fraczek M., Gsaller F., Davies E., Carr P.,
RA   Gago S., Fortune-Grant R., Rahman S., Gilsenan J.M., Houlder E.,
RA   Kowalski C.H., Raj S., Paul S., Cook P., Parker J.E., Kelly S.,
RA   Cramer R.A., Latge J.P., Moye-Rowley S., Bignell E., Bowyer P.,
RA   Bromley M.J.;
RT   "The negative cofactor 2 complex is a key regulator of drug resistance in
RT   Aspergillus fumigatus.";
RL   Nat. Commun. 11:427-427(2020).
CC   -!- FUNCTION: Part of the NCT transcriptional regulatory complex that acts
CC       as a key regulator of ergosterol biosynthesis and the azole exporter
CC       cdr1B (PubMed:31969561). The NCT complex binds the promoters of genes
CC       linked to azole susceptibility, and especially represses the expression
CC       of cdr1B transporter (PubMed:31969561). {ECO:0000269|PubMed:31969561}.
CC   -!- SUBUNIT: Forms the NCT transcriptional regulatory complex with nctA and
CC       mot1. {ECO:0000269|PubMed:31969561}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:31969561}.
CC   -!- DISRUPTION PHENOTYPE: Leads to a multidrug-resistance phenotype,
CC       including the azoles (itraconazole, voriconazole and posaconazole), as
CC       well as the salvage therapeutic amphotericin B and terbinafine, an
CC       agent used in the treatment of chronic and allergic disease
CC       (PubMed:31969561). Leads also to transcriptional derepression of cdr1B
CC       and its over-production (PubMed:31969561). Results also in a notable
CC       increase in the immunogenic properties of Aspergillus fumigatus, but
CC       does not result in loss of virulence (PubMed:31969561).
CC       {ECO:0000269|PubMed:31969561}.
CC   -!- SIMILARITY: Belongs to the NC2 beta/DR1 family. {ECO:0000305}.
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DR   EMBL; AAHF01000010; EAL86519.2; -; Genomic_DNA.
DR   RefSeq; XP_748557.2; XM_743464.2.
DR   AlphaFoldDB; Q4WFF8; -.
DR   SMR; Q4WFF8; -.
DR   STRING; 746128.CADAFUBP00004511; -.
DR   EnsemblFungi; EAL86519; EAL86519; AFUA_3G02340.
DR   GeneID; 3505855; -.
DR   KEGG; afm:AFUA_3G02340; -.
DR   VEuPathDB; FungiDB:Afu3g02340; -.
DR   eggNOG; KOG0871; Eukaryota.
DR   HOGENOM; CLU_066247_11_3_1; -.
DR   InParanoid; Q4WFF8; -.
DR   OMA; KTIAPDH; -.
DR   OrthoDB; 1465912at2759; -.
DR   Proteomes; UP000002530; Chromosome 3.
DR   GO; GO:0017054; C:negative cofactor 2 complex; IBA:GO_Central.
DR   GO; GO:0003682; F:chromatin binding; IEA:EnsemblFungi.
DR   GO; GO:0001046; F:core promoter sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0140223; F:general transcription initiation factor activity; IEA:InterPro.
DR   GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR   GO; GO:0017025; F:TBP-class protein binding; IBA:GO_Central.
DR   GO; GO:0003713; F:transcription coactivator activity; IEA:EnsemblFungi.
DR   GO; GO:0003714; F:transcription corepressor activity; IEA:EnsemblFungi.
DR   GO; GO:0006338; P:chromatin remodeling; IBA:GO_Central.
DR   GO; GO:0017055; P:negative regulation of RNA polymerase II transcription preinitiation complex assembly; IEA:EnsemblFungi.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0016480; P:negative regulation of transcription by RNA polymerase III; IEA:EnsemblFungi.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0061408; P:positive regulation of transcription from RNA polymerase II promoter in response to heat stress; IEA:EnsemblFungi.
DR   GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IBA:GO_Central.
DR   Gene3D; 1.10.20.10; -; 1.
DR   InterPro; IPR003958; CBFA_NFYB_domain.
DR   InterPro; IPR009072; Histone-fold.
DR   InterPro; IPR042225; Ncb2.
DR   PANTHER; PTHR46138; PTHR46138; 1.
DR   Pfam; PF00808; CBFD_NFYB_HMF; 1.
DR   SUPFAM; SSF47113; SSF47113; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..142
FT                   /note="NCT transcriptional regulatory complex subunit B"
FT                   /id="PRO_0000449614"
SQ   SEQUENCE   142 AA;  16096 MW;  A5355151498B8736 CRC64;
     MSDREFSSND DLSLPKATVQ KIITEILPPS SGQTFSKDAR DLLMECCVEF ITLISSEAND
     ISEKEAKKTI ACEHVERALR DLGFGDYIPE VLAVAEEHKE QLKSREKKQS KMEQSGLTEE
     ELLRQQQELF RSATEKYNAA PE