NCX9_CAEEL
ID NCX9_CAEEL Reviewed; 651 AA.
AC O16242;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Mitochondrial sodium/calcium exchanger protein {ECO:0000305};
DE AltName: Full=Sodium/calcium exchanger 9 {ECO:0000303|PubMed:28196860};
DE Flags: Precursor;
GN Name=ncx-9 {ECO:0000303|PubMed:28196860, ECO:0000312|WormBase:C13D9.8};
GN ORFNames=C13D9.8 {ECO:0000312|WormBase:C13D9.8};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, ACTIVITY REGULATION, TISSUE SPECIFICITY,
RP AND MUTAGENESIS OF GLY-205.
RX PubMed=28196860; DOI=10.1074/jbc.m116.758953;
RA Sharma V., Roy S., Sekler I., O'Halloran D.M.;
RT "The NCLX-type Na(+)/Ca(2+) exchanger NCX-9 is required for patterning of
RT neural circuits in Caenorhabditis elegans.";
RL J. Biol. Chem. 292:5364-5377(2017).
CC -!- FUNCTION: Mitochondrial sodium/calcium antiporter that mediates sodium-
CC dependent calcium efflux from mitochondrion, thereby acting as a key
CC regulator of mitochondrion calcium homeostasis (Probable). Required for
CC patterning of neural circuits: functions in the same pathway as RAC-
CC dependent effectors of the unc-6/netrin signaling pathway to set left/
CC right patterning of the VD/DD GABAergic circuit (PubMed:28196860).
CC {ECO:0000269|PubMed:28196860}.
CC -!- ACTIVITY REGULATION: Inhibited by the sodium/calcium exchanger
CC inhibitor CGP-37157. {ECO:0000269|PubMed:28196860}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000305|PubMed:28196860}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in the seam cells of the organism.
CC Expression is visible in the seam cells across all larval stages, and
CC expression persists into the adult stage of the organism.
CC {ECO:0000269|PubMed:28196860}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. SLC24A subfamily. {ECO:0000305}.
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DR EMBL; BX284605; CCD64394.1; -; Genomic_DNA.
DR PIR; T03889; T03889.
DR RefSeq; NP_504342.1; NM_071941.1.
DR AlphaFoldDB; O16242; -.
DR STRING; 6239.C13D9.8; -.
DR TCDB; 2.A.19.4.14; the ca(2+):cation antiporter (caca) family.
DR PaxDb; O16242; -.
DR EnsemblMetazoa; C13D9.8.1; C13D9.8.1; WBGene00003574.
DR GeneID; 182576; -.
DR KEGG; cel:CELE_C13D9.8; -.
DR UCSC; C13D9.8; c. elegans.
DR CTD; 182576; -.
DR WormBase; C13D9.8; CE52885; WBGene00003574; ncx-9.
DR eggNOG; KOG2399; Eukaryota.
DR GeneTree; ENSGT00970000196478; -.
DR HOGENOM; CLU_004979_3_1_1; -.
DR InParanoid; O16242; -.
DR OMA; YPKMAFA; -.
DR OrthoDB; 478735at2759; -.
DR PhylomeDB; O16242; -.
DR PRO; PR:O16242; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00003574; Expressed in adult organism.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0005432; F:calcium:sodium antiporter activity; IDA:UniProtKB.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0099093; P:calcium export from the mitochondrion; IDA:UniProtKB.
DR GO; GO:0006812; P:cation transport; IBA:GO_Central.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IBA:GO_Central.
DR GO; GO:1905815; P:regulation of dorsal/ventral axon guidance; IMP:UniProtKB.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1420.30; -; 2.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR Pfam; PF01699; Na_Ca_ex; 2.
PE 1: Evidence at protein level;
KW Antiport; Calcium; Calcium transport; Ion transport; Membrane;
KW Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Sensory transduction; Sodium; Sodium transport; Transit peptide;
KW Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000305"
FT CHAIN ?..651
FT /note="Mitochondrial sodium/calcium exchanger protein"
FT /id="PRO_0000440955"
FT TOPO_DOM ?..50
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 51..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 72..91
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 113..126
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 148..161
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 183
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 205..398
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 399..419
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 420..428
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 429..449
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 450..458
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 459..479
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 480..486
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 487..507
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 508..510
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 511..531
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 532..559
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 560..580
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 581..595
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 596..616
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 617..626
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 627..647
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 648..651
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT MUTAGEN 205
FT /note="G->E: In gk-773708; development defects in
FT stereotyped left/right axon guidance choices within the
FT GABAergic motor neuron circuit."
FT /evidence="ECO:0000269|PubMed:28196860"
SQ SEQUENCE 651 AA; 73508 MW; 29CED65FDA0221AD CRC64;
MCSSEECLID KSWTSEEKCE YIKCNQDSCE GGGYLTWSHY VKCQYNIGVR VILIILGILY
LIILFVIMSS IADDFFCPAI SGIVSHLRMS ESIAGVTFLA FGNGAPDVFS SISSVLTTPK
PKADLALGDL FGTSIFVTTV VLAIIIFTKS FKVAIIPTLR DLIFYMTTLA FIVFCFLKFD
KIEVWMPATF LGIYGVYVVT VIILGIYRTH RKKRNLKKKN KELEDFLSRP SSAASTTPIF
GAMKLKEETI SVSALFHFMI GYSQFIKNLT RANLKRTTNN NNNDNKNEKR GIVNLGFSEP
YSIPSERKIS TIFKQNTFET DLESLESLAD LDGSDDEGRE EKFGYAHHTV FTSHDQISLV
ASEIEEIEIT TWRSWDWVWD LFNHLKSWPS RDEFSEMNIF IKIVTVIKVV PVFFFKLTVP
SNEMSWCKPL FILHCFASIQ FALFSIQIIT LKPFDGSPGL WLYGLGFSAI LAMVAMYFLP
LSKEQKYYKE IYSYLGFLMS IAWIYATSNE IVSVVTMIGV VTGLSMELLG LTIMAWSNCI
GDIVADIAVV KQGYPKMAMA AAIGGPLFNL LIGFGLPFTI AAAQGKEMEL LINPVYRLLM
LFLGISLVTT FVALFIQRFT VRRPHAVLLI FIFVVFLIFI CLAEFHVLEW N
