NCYM_HUMAN
ID NCYM_HUMAN Reviewed; 109 AA.
AC P40205; Q53TD4;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 2.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=N-cym protein;
DE AltName: Full=N-myc opposite strand;
GN Name=MYCNOS; Synonyms=CYMN, NCYM;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=1419902;
RA Armstrong B.C., Krystal G.W.;
RT "Isolation and characterization of complementary DNA for N-cym, a gene
RT encoded by the DNA strand opposite to N-myc.";
RL Cell Growth Differ. 3:385-390(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INTERACTION WITH
RP MYCN AND GSK3B.
RX PubMed=24391509; DOI=10.1371/journal.pgen.1003996;
RA Suenaga Y., Islam S.M., Alagu J., Kaneko Y., Kato M., Tanaka Y., Kawana H.,
RA Hossain S., Matsumoto D., Yamamoto M., Shoji W., Itami M., Shibata T.,
RA Nakamura Y., Ohira M., Haraguchi S., Takatori A., Nakagawara A.;
RT "NCYM, a Cis-antisense gene of MYCN, encodes a de novo evolved protein that
RT inhibits GSK3beta resulting in the stabilization of MYCN in human
RT neuroblastomas.";
RL PLoS Genet. 10:E1003996-E1003996(2014).
CC -!- FUNCTION: Regulates stability of MYCN in neuroblastoma cells by
CC inhibiting GSK3B-mediated MYCN phosphorylation. Inhibits GSK3B activity
CC by promoting its phosphorylation at 'Ser-9' (PubMed:24391509).
CC {ECO:0000269|PubMed:24391509}.
CC -!- SUBUNIT: Interacts with MYCN and GSK3B. {ECO:0000269|PubMed:24391509}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:24391509}. Nucleus
CC {ECO:0000269|PubMed:24391509}.
CC -!- TISSUE SPECIFICITY: Expressed in the neuronal cells of the cerebrum and
CC cerebellum, spermatocytes of the testis, pancreatic cells and also the
CC heart. Expressed in both primary and metastatic neuroblastomas and in
CC thyroid tumors (at protein level). Expression is associated with poor
CC prognosis in neuroblastoma. Expressed in the fetal brain, lung, liver
CC and kidney at varying low levels. {ECO:0000269|PubMed:1419902,
CC ECO:0000269|PubMed:24391509}.
CC -!- DEVELOPMENTAL STAGE: Expressed during fetal development, as well as in
CC tumor cell lines containing amplified MYCN loci, where it is expressed
CC at very high levels.
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DR EMBL; S49953; AAB24206.1; -; mRNA.
DR EMBL; AC010145; AAY14951.1; -; Genomic_DNA.
DR PIR; A49009; A49009.
DR RefSeq; NP_001316897.1; NM_001329968.1.
DR RefSeq; NP_006307.1; NM_006316.1.
DR AlphaFoldDB; P40205; -.
DR BioMuta; HGNC:16911; -.
DR PeptideAtlas; P40205; -.
DR PRIDE; P40205; -.
DR GeneCards; MYCNOS; -.
DR HGNC; HGNC:16911; MYCNOS.
DR MalaCards; MYCNOS; -.
DR MIM; 605374; gene.
DR neXtProt; NX_P40205; -.
DR OrthoDB; 1477400at2759; -.
DR PathwayCommons; P40205; -.
DR GenomeRNAi; 10408; -.
DR Pharos; P40205; Tdark.
DR PRO; PR:P40205; -.
DR Proteomes; UP000005640; Unplaced.
DR RNAct; P40205; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0033673; P:negative regulation of kinase activity; IDA:UniProtKB.
DR GO; GO:0031647; P:regulation of protein stability; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..109
FT /note="N-cym protein"
FT /id="PRO_0000096769"
FT CONFLICT 70
FT /note="V -> L (in Ref. 1; AAB24206)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 109 AA; 11733 MW; 1F6A1628CF07F6F3 CRC64;
MQHPPCEPGN CLSLKEKKIT EGSGGVCWGG ETDASNPAPA LTACCAAERE ANVEQGLAGR
LLLCNYERRV VRRCKIAGRG RAPLGTRPLD VSSFKLKEEG RPPCLKINK
