ID   NCZS_STRML              Reviewed;         113 AA.
AC   P0A3S0; P01550;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Neocarzinostatin;
DE            Short=NCS;
DE   AltName: Full=Mitomalcin;
DE            Short=MMC;
GN   Name=ncsA;
OS   Streptomyces malayensis.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1918;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=1831044; DOI=10.1021/bi00245a009;
RA   Gao X., Burkhart W.;
RT   "Two- and three-dimensional proton NMR studies of apo-neocarzinostatin.";
RL   Biochemistry 30:7730-7739(1991).
CC   -!- FUNCTION: NCS has antibiotic activity (for Gram-positive bacteria) and
CC       antitumor activity (for certain mouse tumors). NCS binds non-covalently
CC       to a chromophore which is the cytotoxic and mutagenic component of the
CC       antibiotic. The chromophore binds to DNA as a weak intercalator and
CC       causes single- and double-strand breaks.
CC   -!- SIMILARITY: Belongs to the neocarzinostatin family. {ECO:0000305}.
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DR   PIR; B40278; B40278.
DR   PDB; 4JW3; X-ray; 2.60 A; A/B=1-112.
DR   PDBsum; 4JW3; -.
DR   AlphaFoldDB; P0A3S0; -.
DR   BMRB; P0A3S0; -.
DR   SMR; P0A3S0; -.
DR   DrugBank; DB04408; Ncs-Chromophore.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR027273; Neocarzinostatin-like.
DR   InterPro; IPR002186; Neocarzinostatin_fam.
DR   Pfam; PF00960; Neocarzinostat; 1.
DR   PRINTS; PR01885; MACROMOMYCIN.
DR   SUPFAM; SSF49319; SSF49319; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic; Antimicrobial; Direct protein sequencing;
KW   Disulfide bond; DNA-binding.
FT   CHAIN           1..113
FT                   /note="Neocarzinostatin"
FT                   /id="PRO_0000213118"
FT   DISULFID        37..47
FT                   /evidence="ECO:0000250"
FT   DISULFID        88..93
FT                   /evidence="ECO:0000250"
FT   STRAND          4..9
FT                   /evidence="ECO:0007829|PDB:4JW3"
FT   STRAND          18..25
FT                   /evidence="ECO:0007829|PDB:4JW3"
FT   STRAND          31..41
FT                   /evidence="ECO:0007829|PDB:4JW3"
FT   STRAND          44..56
FT                   /evidence="ECO:0007829|PDB:4JW3"
FT   STRAND          63..68
FT                   /evidence="ECO:0007829|PDB:4JW3"
FT   STRAND          71..76
FT                   /evidence="ECO:0007829|PDB:4JW3"
FT   STRAND          82..87
FT                   /evidence="ECO:0007829|PDB:4JW3"
FT   TURN            88..90
FT                   /evidence="ECO:0007829|PDB:4JW3"
FT   STRAND          91..98
FT                   /evidence="ECO:0007829|PDB:4JW3"
FT   STRAND          100..102
FT                   /evidence="ECO:0007829|PDB:4JW3"
SQ   SEQUENCE   113 AA;  11096 MW;  5A2DDA5014EE882F CRC64;
     AAPTATVTPS SGLSDGTVVK VAGAGLQAGT AYDVGQCAWV DTGVLACNPA DFSSVTADAN
     GSASTSLTVR RSFEGFLFDG TRWGTVDCTT AACQVGLSDA AGNGPEGVAI SFN