ID   NDB26_ISOMC             Reviewed;          56 AA.
AC   P0CF38;
DT   20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT   20-APR-2010, sequence version 1.
DT   25-MAY-2022, entry version 20.
DE   RecName: Full=Im-1 {ECO:0000303|PubMed:20139620};
DE   AltName: Full=Non-disulfide-bridged peptide 2.6 {ECO:0000303|PubMed:24184590};
DE            Short=NDBP-2.6 {ECO:0000303|PubMed:24184590};
OS   Isometrus maculatus (Lesser brown scorpion) (Scorpio maculatus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Isometrus.
OX   NCBI_TaxID=497827;
RN   [1]
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, FUNCTION, TOXIC DOSE, SYNTHESIS, AND
RP   SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=20139620; DOI=10.1271/bbb.90723;
RA   Miyashita M., Sakai A., Matsushita N., Hanai Y., Nakagawa Y., Miyagawa H.;
RT   "A novel amphipathic linear peptide with both insect toxicity and
RT   antimicrobial activity from the venom of the scorpion Isometrus
RT   maculatus.";
RL   Biosci. Biotechnol. Biochem. 74:364-369(2010).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=24184590; DOI=10.1016/j.peptides.2013.10.021;
RA   Almaaytah A., Albalas Q.;
RT   "Scorpion venom peptides with no disulfide bridges: a review.";
RL   Peptides 51:35-45(2014).
CC   -!- FUNCTION: Causes cytolysis by the formation of pores in the membrane.
CC       Causes rapid and reversible paralysis in crickets. Has antibacterial
CC       activity on the Gram-negative bacterium E.coli (NBRC 3972) (MIC=0.4-0.8
CC       uM), and the Gram-positive bacteria S.aureus (NBRC 13276) (MIC=13-25
CC       uM) and B.subtilis (NBRC 3009) (MIC=0.8-1.6 uM).
CC       {ECO:0000269|PubMed:20139620}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20139620}. Target
CC       cell membrane {ECO:0000305|PubMed:20139620}. Note=Forms a helical
CC       membrane channel in the prey. {ECO:0000305|PubMed:20139620}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=6344.5; Method=Electrospray; Note=Monoisotopic
CC       mass.; Evidence={ECO:0000269|PubMed:20139620};
CC   -!- TOXIC DOSE: PD(50) is 38 mg/kg into the insect A.domestica.
CC       {ECO:0000269|PubMed:20139620}.
CC   -!- SIMILARITY: Belongs to the non-disulfide-bridged peptide (NDBP)
CC       superfamily. Long chain multifunctional peptide (group 2) family.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P0CF38; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Cytolysis; Direct protein sequencing;
KW   Ion transport; Membrane; Secreted; Target cell membrane; Target membrane;
KW   Toxin; Transmembrane; Transport.
FT   CHAIN           1..56
FT                   /note="Im-1"
FT                   /evidence="ECO:0000269|PubMed:20139620"
FT                   /id="PRO_0000393446"
SQ   SEQUENCE   56 AA;  6348 MW;  CABDD8F97AA9FE96 CRC64;
     FSFKRLKGFA KKLWNSKLAR KIRTKGLKYV KNFAKDMLSE GEEAPPAAEP PVEAPQ