ID   NDB28_ANDMU             Reviewed;          73 AA.
AC   N0EAL3;
DT   04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT   26-JUN-2013, sequence version 1.
DT   25-MAY-2022, entry version 13.
DE   RecName: Full=Mauriporin {ECO:0000303|Ref.1};
DE   AltName: Full=Non-disulfide-bridged peptide 2.8 {ECO:0000303|PubMed:24184590};
DE            Short=NDBP-2.8 {ECO:0000303|PubMed:24184590};
DE   Flags: Precursor;
OS   Androctonus mauritanicus (Fat-tailed scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Androctonus.
OX   NCBI_TaxID=6859;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SYNTHESIS OF 23-70, FUNCTION, MASS
RP   SPECTROMETRY, SUBCELLULAR LOCATION, AND CIRCULAR DICHROISM.
RC   TISSUE=Venom, and Venom gland;
RA   Almaaytah A., Tarazi S., Mhaidat N., Al Balas Q., Mukattash T.L.;
RT   "Mauriporin, a novel cationic alpha-helical peptide with selective
RT   cytotoxic activity against prostate cancer cell lines from the venom of the
RT   scorpion Androctonus mauritanicus.";
RL   Int. J. Pept. Res. Ther. 4:281-293(2013).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=24184590; DOI=10.1016/j.peptides.2013.10.021;
RA   Almaaytah A., Albalas Q.;
RT   "Scorpion venom peptides with no disulfide bridges: a review.";
RL   Peptides 51:35-45(2014).
CC   -!- FUNCTION: Amphipathic peptide that exerts potent selective cytotoxic
CC       and antiproliferative activity against three different prostate cancer
CC       cell lines (IC(50)=4.4-7.8 uM), compared to non-tumorigenic cells
CC       (IC(50)=59.7 in Vero and 62.5 in HUVEC cells). This peptide possibly
CC       exerts its cytotoxic activity through a necrotic mode of cell death.
CC       {ECO:0000269|Ref.1}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}. Target cell
CC       membrane {ECO:0000305|Ref.1}. Note=Forms a helical membrane channel in
CC       the prey. {ECO:0000305|Ref.1}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=5398.1; Method=MALDI;
CC       Evidence={ECO:0000269|Ref.1};
CC   -!- MISCELLANEOUS: Only shows diminished hemolytic activity against sheep
CC       erythrocytes. Does not induce cell death through apoptosis and
CC       consequently is not acting upon an intracellular target.
CC       {ECO:0000269|Ref.1}.
CC   -!- SIMILARITY: Belongs to the non-disulfide-bridged peptide (NDBP)
CC       superfamily. Long chain multifunctional peptide (group 2) family.
CC       {ECO:0000305}.
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DR   EMBL; HF545613; CCN80315.1; -; mRNA.
DR   AlphaFoldDB; N0EAL3; -.
DR   SMR; N0EAL3; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Cleavage on pair of basic residues; Membrane;
KW   Secreted; Signal; Target cell membrane; Target membrane; Toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..70
FT                   /note="Mauriporin"
FT                   /id="PRO_0000432337"
SQ   SEQUENCE   73 AA;  8417 MW;  F07132E1C03B6BD0 CRC64;
     MNKKTLLVIF FITMLIVDEV NSFKIGGFIK KLWRSKLAKK LRAKGRELLK DYANRVINGG
     PEEEAAVPAE RRR