ID   NDB2T_OPICA             Reviewed;          61 AA.
AC   Q5VJS9;
DT   03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Opistoporin-4 {ECO:0000303|Ref.1};
DE   Flags: Precursor;
OS   Opistophthalmus carinatus (African yellow leg scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Iurida; Scorpionoidea; Scorpionidae; Opistophthalminae;
OC   Opistophthalmus.
OX   NCBI_TaxID=190115;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RA   Zhu S., Tytgat J.;
RT   "Precursor organization and gene structure of scorpion venom antimicrobial
RT   peptides.";
RL   Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: At high concentrations, acts as pore former in cellular
CC       membranes and causes the leakage of the cells. At submicromolar
CC       concentrations, degranulates granulocytes and has a weak hemolytic
CC       activity against human erythrocytes. Also strongly inhibits the
CC       production of superoxide anions. Has a strong antibacterial activity
CC       against Gram-negative bacteria but is less active against Gram-positive
CC       bacteria. Also has antifungal activity. {ECO:0000250|UniProtKB:P83313}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P83313}. Target
CC       cell membrane {ECO:0000250|UniProtKB:P83313}. Note=Forms a helical
CC       membrane channel in the prey. {ECO:0000250|UniProtKB:P83313}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|Ref.1}.
CC   -!- SIMILARITY: Belongs to the non-disulfide-bridged peptide (NDBP)
CC       superfamily. Long chain multifunctional peptide (group 2) family.
CC       {ECO:0000305}.
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DR   EMBL; AY427949; AAQ94361.1; -; mRNA.
DR   AlphaFoldDB; Q5VJS9; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR012526; Antimicrobial_7.
DR   Pfam; PF08102; Antimicrobial_7; 1.
PE   3: Inferred from homology;
KW   Antibiotic; Antimicrobial; Cytolysis; Fungicide; Hemolysis; Ion transport;
KW   Membrane; Secreted; Target cell membrane; Target membrane; Transmembrane;
KW   Transport.
FT   CHAIN           1..44
FT                   /note="Opistoporin-4"
FT                   /evidence="ECO:0000305|Ref.1"
FT                   /id="PRO_5000092331"
FT   PROPEP          45..61
FT                   /evidence="ECO:0000250|UniProtKB:P83313"
FT                   /id="PRO_0000366113"
SQ   SEQUENCE   61 AA;  6886 MW;  4E0D1276D1B92D7B CRC64;
     GKVWDWIKKT AKDVLNSDVA KQLKNKALNA AKNFVAEKIG ATPSEAGQMP FDEFMDILHY
     Y