ID   NDB43_MESMA             Reviewed;          74 AA.
AC   Q718F4; Q2M5D6; Q95P85;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Peptide BmKb1;
DE   AltName: Full=Antimicrobial peptide-like protein;
DE   AltName: Full=BmKb1';
DE   AltName: Full=BmKb1*;
DE   AltName: Full=Non-disulfide-bridged peptide 4.2 {ECO:0000303|PubMed:16036557};
DE            Short=NDBP-4.2 {ECO:0000303|PubMed:16036557};
DE   AltName: Full=Non-disulfide-bridged peptide 4.3 {ECO:0000303|PubMed:24184590};
DE            Short=NDBP-4.3 {ECO:0000303|PubMed:24184590};
DE   AltName: Full=Toxin peptide 6;
DE   Flags: Precursor;
OS   Mesobuthus martensii (Manchurian scorpion) (Buthus martensii).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Mesobuthus.
OX   NCBI_TaxID=34649;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SYNTHESIS OF 24-40, AMIDATION AT LYS-40, AND
RP   FUNCTION.
RC   TISSUE=Venom gland;
RX   PubMed=15062994; DOI=10.1016/j.peptides.2003.12.003;
RA   Zeng X.-C., Wang S.-X., Zhu Y., Zhu S.-Y., Li W.-X.;
RT   "Identification and functional characterization of novel scorpion venom
RT   peptides with no disulfide bridge from Buthus martensii Karsch.";
RL   Peptides 25:143-150(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=16040157; DOI=10.1016/j.peptides.2005.06.008;
RA   Luo F., Zeng X.-C., Hahin R., Cao Z.-J., Liu H., Li W.-X.;
RT   "Genomic organization of four novel nondisulfide-bridged peptides from
RT   scorpion Mesobuthus martensii Karsch: gaining insight into evolutionary
RT   mechanism.";
RL   Peptides 26:2427-2433(2005).
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=16036557; DOI=10.1080/15216540500058899;
RA   Zeng X.C., Corzo G., Hahin R.;
RT   "Scorpion venom peptides without disulfide bridges.";
RL   IUBMB Life 57:13-21(2005).
RN   [4]
RP   NOMENCLATURE.
RX   PubMed=24184590; DOI=10.1016/j.peptides.2013.10.021;
RA   Almaaytah A., Albalas Q.;
RT   "Scorpion venom peptides with no disulfide bridges: a review.";
RL   Peptides 51:35-45(2014).
CC   -!- FUNCTION: Has antibacterial activity against Gram-positive bacteria
CC       S.aureus, M.luteus, B.subtilis, and Gram-negative bacteria E.coli, and
CC       P.aeruginosa. {ECO:0000269|PubMed:15062994}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Target cell membrane
CC       {ECO:0000250}. Note=Forms a helical membrane channel in the prey.
CC       {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the non-disulfide-bridged peptide (NDBP)
CC       superfamily. Short antimicrobial peptide (group 4) family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK61826.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AF543048; AAQ11422.1; -; mRNA.
DR   EMBL; AF159979; AAK61826.1; ALT_SEQ; mRNA.
DR   EMBL; AY700566; AAW23032.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q718F4; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Antibiotic; Antimicrobial; Cleavage on pair of basic residues;
KW   Membrane; Secreted; Signal; Target cell membrane; Target membrane;
KW   Transmembrane.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PEPTIDE         23..40
FT                   /note="Peptide BmKb1"
FT                   /id="PRO_0000231496"
FT   PROPEP          46..74
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000231497"
FT   MOD_RES         40
FT                   /note="Lysine amide"
FT                   /evidence="ECO:0000269|PubMed:15062994"
FT   CONFLICT        50..74
FT                   /note="Missing (in Ref. 1; AAK61826)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        50
FT                   /note="Y -> Q (in Ref. 2; AAW23032)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   74 AA;  8616 MW;  C0F93E0F23AD8B82 CRC64;
     MEIKYLLTVF LVLLIVSDHC QAFLFSLIPS AISGLISAFK GRRKRDLNGY IDHFKNFRKR
     DAELEELLSK LPIY