ID   NDB47_ISOMC             Reviewed;          74 AA.
AC   C7B247;
DT   20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT   22-SEP-2009, sequence version 1.
DT   03-AUG-2022, entry version 21.
DE   RecName: Full=Imcroporin {ECO:0000303|PubMed:28941793};
DE   AltName: Full=Non-disulfide-bridged peptide 4.7 {ECO:0000303|PubMed:24184590};
DE            Short=NDBP-4.7 {ECO:0000303|PubMed:24184590};
DE   Flags: Precursor;
OS   Isometrus maculatus (Lesser brown scorpion) (Scorpio maculatus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Isometrus.
OX   NCBI_TaxID=497827;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SYNTHESIS, FUNCTION, AND TISSUE SPECIFICITY.
RC   TISSUE=Venom gland;
RX   PubMed=19451300; DOI=10.1128/aac.01436-08;
RA   Zhao Z., Ma Y., Dai C., Zhao R., Li S., Wu Y., Cao Z., Li W.;
RT   "Imcroporin, a new cationic antimicrobial peptide from the venom of the
RT   scorpion Isometrus maculates.";
RL   Antimicrob. Agents Chemother. 53:3472-3477(2009).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=24184590; DOI=10.1016/j.peptides.2013.10.021;
RA   Almaaytah A., Albalas Q.;
RT   "Scorpion venom peptides with no disulfide bridges: a review.";
RL   Peptides 51:35-45(2014).
RN   [3]
RP   PROTEIN SEQUENCE OF 23-39, SUBCELLULAR LOCATION, MASS SPECTROMETRY,
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND AMIDATION AT LYS-39.
RC   TISSUE=Venom {ECO:0000303|PubMed:28941793};
RX   PubMed=28941793; DOI=10.1016/j.toxicon.2017.09.010;
RA   Miyashita M., Kitanaka A., Yakio M., Yamazaki Y., Nakagawa Y., Miyagawa H.;
RT   "Complete de novo sequencing of antimicrobial peptides in the venom of the
RT   scorpion Isometrus maculatus.";
RL   Toxicon 139:1-12(2017).
CC   -!- FUNCTION: Has potent antibacterial activity against Gram-positive
CC       bacteria M.luteus, B.thuringiensis, S.aureus and B.subtilis, but not
CC       Gram-negative bacteria. Shows a weak cytotoxicity effect against
CC       mammalian cell lines and relatively low hemolytic activity against
CC       human erythrocytes. {ECO:0000269|PubMed:19451300}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:28941793}. Target
CC       cell membrane {ECO:0000305|PubMed:28941793}. Note=Probably forms a
CC       helical membrane channel in the prey. {ECO:0000305|PubMed:28941793}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:19451300}.
CC   -!- MASS SPECTROMETRY: Mass=1760.0; Method=MALDI; Note=Amidated.;
CC       Evidence={ECO:0000269|PubMed:28941793};
CC   -!- MASS SPECTROMETRY: Mass=1761.0; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:28941793};
CC   -!- MISCELLANEOUS: A fragment comprising residues 4-17 has been detected in
CC       venom but it is unclear whether it has a physiological role or is
CC       simply due to degradation. {ECO:0000305|PubMed:28941793}.
CC   -!- SIMILARITY: Belongs to the non-disulfide-bridged peptide (NDBP)
CC       superfamily. Short antimicrobial peptide (group 4) family.
CC       {ECO:0000305}.
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DR   EMBL; FJ750949; ACT78488.1; -; mRNA.
DR   AlphaFoldDB; C7B247; -.
DR   SMR; C7B247; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Antibiotic; Antimicrobial; Cleavage on pair of basic residues;
KW   Direct protein sequencing; Membrane; Secreted; Signal;
KW   Target cell membrane; Target membrane.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000269|PubMed:28941793"
FT   PEPTIDE         23..39
FT                   /note="Imcroporin"
FT                   /evidence="ECO:0000269|PubMed:28941793"
FT                   /id="PRO_0000393447"
FT   PROPEP          45..74
FT                   /evidence="ECO:0000305|PubMed:28941793"
FT                   /id="PRO_0000393448"
FT   MOD_RES         39
FT                   /note="Lysine amide; partial"
FT                   /evidence="ECO:0000269|PubMed:28941793"
SQ   SEQUENCE   74 AA;  8777 MW;  CFF4AE6A2C2F1529 CRC64;
     MKFQYLLAVF LIVLVVTDHC QAFFSLLPSL IGGLVSAIKG RRRRQLEARF EPKQRNFRKR
     ELDFEKLFAN MPDY