NDB4A_ISOMC
ID NDB4A_ISOMC Reviewed; 17 AA.
AC C0HL59;
DT 31-JAN-2018, integrated into UniProtKB/Swiss-Prot.
DT 31-JAN-2018, sequence version 1.
DT 25-MAY-2022, entry version 8.
DE RecName: Full=Peptide Im-4 {ECO:0000303|PubMed:28941793};
OS Isometrus maculatus (Lesser brown scorpion) (Scorpio maculatus).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC Scorpiones; Buthida; Buthoidea; Buthidae; Isometrus.
OX NCBI_TaxID=497827 {ECO:0000303|PubMed:28941793};
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY,
RP IDENTIFICATION BY MASS SPECTROMETRY, AND AMIDATION AT LYS-17.
RC TISSUE=Venom {ECO:0000303|PubMed:28941793};
RX PubMed=28941793; DOI=10.1016/j.toxicon.2017.09.010;
RA Miyashita M., Kitanaka A., Yakio M., Yamazaki Y., Nakagawa Y., Miyagawa H.;
RT "Complete de novo sequencing of antimicrobial peptides in the venom of the
RT scorpion Isometrus maculatus.";
RL Toxicon 139:1-12(2017).
CC -!- FUNCTION: Probably forms pores in target membranes. Has antibacterial
CC activity against Gram-positive bacteria S.aureus NBRC 13276 (MIC=5-10
CC uM) and B.subtilis NBRC 3009 (MIC=2.5-5 uM) but not against Gram-
CC negative bacterium E.coli NBRC 3972. {ECO:0000269|PubMed:28941793}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:28941793}. Target
CC cell membrane {ECO:0000305|PubMed:28941793}. Note=Probably forms a
CC helical membrane channel in the prey. {ECO:0000305|PubMed:28941793}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:28941793}.
CC -!- MASS SPECTROMETRY: Mass=1714.0; Method=MALDI; Note=Amidated.;
CC Evidence={ECO:0000269|PubMed:28941793};
CC -!- MASS SPECTROMETRY: Mass=1714.9; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:28941793};
CC -!- MISCELLANEOUS: Fragments comprising residues 4-17 and 5-17 have been
CC detected in venom but it is unclear whether they have a physiological
CC role or are simply due to degradation. {ECO:0000305|PubMed:28941793}.
CC -!- SIMILARITY: Belongs to the non-disulfide-bridged peptide (NDBP)
CC superfamily. Short antimicrobial peptide (group 4) family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR AlphaFoldDB; C0HL59; -.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR GO; GO:0031640; P:killing of cells of another organism; IDA:UniProtKB.
PE 1: Evidence at protein level;
KW Amidation; Antibiotic; Antimicrobial; Direct protein sequencing; Membrane;
KW Secreted; Target cell membrane; Target membrane.
FT PEPTIDE 1..17
FT /note="Peptide Im-4"
FT /evidence="ECO:0000269|PubMed:28941793"
FT /id="PRO_0000442987"
FT MOD_RES 17
FT /note="Lysine amide; partial"
FT /evidence="ECO:0000269|PubMed:28941793"
SQ SEQUENCE 17 AA; 1700 MW; 6CB4561BFA2546B9 CRC64;
FIGMIPGLIG GLISAIK