NDB4B_ISOMC
ID NDB4B_ISOMC Reviewed; 25 AA.
AC C0HL58;
DT 31-JAN-2018, integrated into UniProtKB/Swiss-Prot.
DT 31-JAN-2018, sequence version 1.
DT 25-MAY-2022, entry version 13.
DE RecName: Full=Peptide Im-5 {ECO:0000303|PubMed:28941793};
OS Isometrus maculatus (Lesser brown scorpion) (Scorpio maculatus).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC Scorpiones; Buthida; Buthoidea; Buthidae; Isometrus.
OX NCBI_TaxID=497827;
RN [1]
RP PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY, TOXIC
RP DOSE, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Venom;
RX PubMed=28941793; DOI=10.1016/j.toxicon.2017.09.010;
RA Miyashita M., Kitanaka A., Yakio M., Yamazaki Y., Nakagawa Y., Miyagawa H.;
RT "Complete de novo sequencing of antimicrobial peptides in the venom of the
RT scorpion Isometrus maculatus.";
RL Toxicon 139:1-12(2017).
CC -!- FUNCTION: Probably forms pores in target membranes. Has antibacterial
CC activity against Gram-negative bacterium E.coli NBRC 3972 (MIC=10 uM)
CC and against Gram-positive bacteria S.aureus NBRC 13276 (MIC=2.5-5 uM)
CC and B.subtilis NBRC 3009 (MIC=0.5-1 uM). Toxic to cricket A.domestica.
CC Has hemolytic activity against sheep erythrocytes.
CC {ECO:0000269|PubMed:28941793}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:28941793}. Target
CC cell membrane {ECO:0000305|PubMed:28941793}. Note=Probably forms a
CC helical membrane channel in the prey. {ECO:0000305|PubMed:28941793}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:28941793}.
CC -!- MASS SPECTROMETRY: Mass=2803.7; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:28941793};
CC -!- TOXIC DOSE: LD(50) is 61 nmol/g by intraabdominal injection into
CC cricket A.domestica. {ECO:0000269|PubMed:28941793}.
CC -!- MISCELLANEOUS: Fragments comprising residues 10-25, 1-19, 7-25, 1-22,
CC 4-25 and 1-23 have been detected in venom but it is unclear whether
CC they have a physiological role or are simply due to degradation.
CC {ECO:0000305|PubMed:28941793}.
CC -!- SIMILARITY: Belongs to the non-disulfide-bridged peptide (NDBP)
CC superfamily. Medium-length antimicrobial peptide (group 3) family.
CC {ECO:0000305}.
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DR AlphaFoldDB; C0HL58; -.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IDA:UniProtKB.
DR GO; GO:0051715; P:cytolysis in another organism; IDA:UniProtKB.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IDA:UniProtKB.
DR InterPro; IPR012523; Antimicrobial_4.
DR Pfam; PF08024; Antimicrobial_4; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Cytolysis; Direct protein sequencing; Hemolysis;
KW Membrane; Secreted; Target cell membrane; Target membrane.
FT PEPTIDE 1..25
FT /note="Peptide Im-5"
FT /evidence="ECO:0000269|PubMed:28941793"
FT /id="PRO_0000442986"
SQ SEQUENCE 25 AA; 2805 MW; EF61297EB0D8D6F1 CRC64;
FLGSLFSIGS KLLPGVIKLF QRKKQ