ID   NDB4F_SCOTI             Reviewed;          69 AA.
AC   P0DJO4;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2012, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=Amphipathic peptide CT2;
DE            Short=StCT2;
DE   AltName: Full=Non-disulfide-bridged peptide 4.15 {ECO:0000303|PubMed:24184590};
DE            Short=NDBP-4.15 {ECO:0000303|PubMed:24184590};
DE   AltName: Full=Non-disulfide-bridged peptide 5.17 {ECO:0000303|PubMed:23624072};
DE            Short=NDBP-5.17 {ECO:0000303|PubMed:23624072};
DE   Flags: Precursor;
OS   Scorpiops tibetanus (Scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Iurida; Chactoidea; Euscorpiidae; Scorpiopinae; Scorpiopini;
OC   Scorpiops.
OX   NCBI_TaxID=500600;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SYNTHESIS OF 24-37, FUNCTION, SUBCELLULAR
RP   LOCATION, AND CIRCULAR DICHROISM ANALYSIS.
RC   TISSUE=Venom gland;
RX   PubMed=22542475; DOI=10.1016/j.peptides.2012.04.010;
RA   Cao L., Li Z., Zhang R., Wu Y., Li W., Cao Z.;
RT   "StCT2, a new antibacterial peptide characterized from the venom of the
RT   scorpion Scorpiops tibetanus.";
RL   Peptides 36:213-220(2012).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=23624072; DOI=10.1016/j.peptides.2013.03.026;
RA   Zeng X.C., Zhou L., Shi W., Luo X., Zhang L., Nie Y., Wang J., Wu S.,
RA   Cao B., Cao H.;
RT   "Three new antimicrobial peptides from the scorpion Pandinus imperator.";
RL   Peptides 45:28-34(2013).
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=24184590; DOI=10.1016/j.peptides.2013.10.021;
RA   Almaaytah A., Albalas Q.;
RT   "Scorpion venom peptides with no disulfide bridges: a review.";
RL   Peptides 51:35-45(2014).
CC   -!- FUNCTION: Antimicrobial peptide that is rapidly bactericidal against
CC       Gram-positive bacteria. {ECO:0000269|PubMed:22542475}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Target cell membrane
CC       {ECO:0000269|PubMed:22542475}. Note=Forms a helical membrane channel in
CC       the prey.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- MISCELLANEOUS: Is highly capable of inhibiting antibiotic-resistant
CC       pathogen growth, including methicillin-resistant S.aureus. In vivo,
CC       shows high antimicrobial activity on a S.aureus-infected mouse model
CC       (PubMed:22542475). {ECO:0000305|PubMed:22542475}.
CC   -!- SIMILARITY: Belongs to the non-disulfide-bridged peptide (NDBP)
CC       superfamily. Short antimicrobial peptide (group 4) family.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P0DJO4; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Amidation; Antibiotic; Antimicrobial; Cleavage on pair of basic residues;
KW   Cytolysis; Hemolysis; Membrane; Secreted; Signal; Target cell membrane;
KW   Target membrane; Transmembrane.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000250"
FT   PEPTIDE         24..37
FT                   /note="Amphipathic peptide CT2"
FT                   /id="PRO_0000418787"
FT   PROPEP          41..69
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000418788"
FT   MOD_RES         37
FT                   /note="Isoleucine amide"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   69 AA;  7930 MW;  36C9AEB21F9AE5AA CRC64;
     MKTQFAVLII SMILMQMLVQ TEAGFWGKLW EGVKSAIGKR SLRNQDQFDN MFDSDLSDAD
     LKLLDDLFD