ID   NDB4G_UROYA             Reviewed;          79 AA.
AC   L0GCV8;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2013, sequence version 1.
DT   25-MAY-2022, entry version 13.
DE   RecName: Full=Antimicrobial peptide UyCT1 {ECO:0000303|PubMed:23182832};
DE            Short=CT1 {ECO:0000303|PubMed:23182832};
DE   AltName: Full=Non-disulfide-bridged peptide 4.16 {ECO:0000303|PubMed:24184590};
DE            Short=NDBP-4.16 {ECO:0000303|PubMed:24184590};
DE   AltName: Full=Non-disulfide-bridged peptide 5.18 {ECO:0000303|PubMed:23624072};
DE            Short=NDBP-5.18 {ECO:0000303|PubMed:23624072};
DE   Contains:
DE     RecName: Full=Antimicrobial peptide UyCT2;
DE     AltName: Full=Non-disulfide-bridged peptide 5.19 {ECO:0000303|PubMed:23624072};
DE              Short=NDBP-5.19 {ECO:0000303|PubMed:23624072};
DE   Flags: Precursor;
OS   Urodacus yaschenkoi (Inland robust scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Iurida; Scorpionoidea; Scorpionidae; Urodacinae; Urodacus.
OX   NCBI_TaxID=1273102;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SYNTHESIS OF 24-37 (CT1), SYNTHESIS OF 25-37
RP   (CT2), AND FUNCTION.
RC   TISSUE=Venom gland;
RX   PubMed=23182832; DOI=10.1016/j.toxicon.2012.11.017;
RA   Luna-Ramirez K., Quintero-Hernandez V., Vargas-Jaimes L., Batista C.V.,
RA   Winkel K.D., Possani L.D.;
RT   "Characterization of the venom from the Australian scorpion Urodacus
RT   yaschenkoi: molecular mass analysis of components, cDNA sequences and
RT   peptides with antimicrobial activity.";
RL   Toxicon 63:44-54(2013).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=23624072; DOI=10.1016/j.peptides.2013.03.026;
RA   Zeng X.C., Zhou L., Shi W., Luo X., Zhang L., Nie Y., Wang J., Wu S.,
RA   Cao B., Cao H.;
RT   "Three new antimicrobial peptides from the scorpion Pandinus imperator.";
RL   Peptides 45:28-34(2013).
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=24184590; DOI=10.1016/j.peptides.2013.10.021;
RA   Almaaytah A., Albalas Q.;
RT   "Scorpion venom peptides with no disulfide bridges: a review.";
RL   Peptides 51:35-45(2014).
CC   -!- FUNCTION: [Antimicrobial peptide UyCT1]: Inhibits the growth of Gram-
CC       positive (S.aureus, MIC=15 uM) and Gram-negative bacteria (E.coli,
CC       MIC=10 uM and P.aeruginosa, MIC=10 uM). It also shows 26% of hemolysis
CC       when 15 uM are tested (81% at 50 uM) (PubMed:23182832).
CC       {ECO:0000269|PubMed:23182832}.
CC   -!- FUNCTION: [Antimicrobial peptide UyCT2]: Inhibits the growth of Gram-
CC       negative bacteria (E.coli, MIC=25 uM and P.aeruginosa, MIC=40 uM). It
CC       also shows 7% of hemolysis when 50 uM are tested. Does not show
CC       activity against the Gram-positive bacteria S.aureus (PubMed:23182832).
CC       {ECO:0000269|PubMed:23182832}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Target cell membrane
CC       {ECO:0000250}. Note=Forms a helical membrane channel in the prey.
CC       {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- SIMILARITY: Belongs to the non-disulfide-bridged peptide (NDBP)
CC       superfamily. Short antimicrobial peptide (group 4) family.
CC       {ECO:0000305}.
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DR   EMBL; JX274240; AGA82754.1; -; mRNA.
DR   AlphaFoldDB; L0GCV8; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Amidation; Antibiotic; Antimicrobial; Cleavage on pair of basic residues;
KW   Cytolysis; Hemolysis; Membrane; Secreted; Signal; Target cell membrane;
KW   Target membrane; Transmembrane.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   PEPTIDE         24..37
FT                   /note="Antimicrobial peptide UyCT1"
FT                   /id="PRO_5001091946"
FT   PEPTIDE         25..37
FT                   /note="Antimicrobial peptide UyCT2"
FT                   /id="PRO_5001091945"
FT   PROPEP          41..79
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000430183"
FT   MOD_RES         37
FT                   /note="Isoleucine amide"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   79 AA;  8765 MW;  44788B738FCD44C9 CRC64;
     MKTQLAFLAI TVILMQLFAQ TEAGFWGKLW EGVKNAIGKR GLRNVDQIAD LFDSGLSDAD
     DLFDSGLSDA DAKFMKMFM