NDB4H_UROYA
ID NDB4H_UROYA Reviewed; 68 AA.
AC L0GCI6;
DT 03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2013, sequence version 1.
DT 25-MAY-2022, entry version 13.
DE RecName: Full=Antimicrobial peptide UyCT3 {ECO:0000303|PubMed:23182832};
DE Short=CT3 {ECO:0000303|PubMed:23182832};
DE AltName: Full=Non-disulfide-bridged peptide 4.17 {ECO:0000303|PubMed:24184590};
DE Short=NDBP-4.17 {ECO:0000303|PubMed:24184590};
DE AltName: Full=Non-disulfide-bridged peptide 5.15 {ECO:0000303|PubMed:23624072};
DE Short=NDBP-5.15 {ECO:0000303|PubMed:23624072};
DE Flags: Precursor;
OS Urodacus yaschenkoi (Inland robust scorpion).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC Scorpiones; Iurida; Scorpionoidea; Scorpionidae; Urodacinae; Urodacus.
OX NCBI_TaxID=1273102;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SYNTHESIS OF 24-36, FUNCTION, MASS
RP SPECTROMETRY, AND AMIDATION AT PHE-36.
RC TISSUE=Venom, and Venom gland;
RX PubMed=23182832; DOI=10.1016/j.toxicon.2012.11.017;
RA Luna-Ramirez K., Quintero-Hernandez V., Vargas-Jaimes L., Batista C.V.,
RA Winkel K.D., Possani L.D.;
RT "Characterization of the venom from the Australian scorpion Urodacus
RT yaschenkoi: molecular mass analysis of components, cDNA sequences and
RT peptides with antimicrobial activity.";
RL Toxicon 63:44-54(2013).
RN [2]
RP NOMENCLATURE.
RX PubMed=23624072; DOI=10.1016/j.peptides.2013.03.026;
RA Zeng X.C., Zhou L., Shi W., Luo X., Zhang L., Nie Y., Wang J., Wu S.,
RA Cao B., Cao H.;
RT "Three new antimicrobial peptides from the scorpion Pandinus imperator.";
RL Peptides 45:28-34(2013).
RN [3]
RP NOMENCLATURE.
RX PubMed=24184590; DOI=10.1016/j.peptides.2013.10.021;
RA Almaaytah A., Albalas Q.;
RT "Scorpion venom peptides with no disulfide bridges: a review.";
RL Peptides 51:35-45(2014).
CC -!- FUNCTION: Antimicrobial peptide that inhibits the growth of Gram-
CC positive (S.aureus, MIC=10 uM) and Gram-negative bacteria (E.coli,
CC MIC=15 uM and P.aeruginosa, MIC=6 uM). It also shows 35% of hemolysis
CC when 15 uM are tested (95% at 50 uM). {ECO:0000269|PubMed:23182832}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Target cell membrane
CC {ECO:0000250}. Note=Forms a helical membrane channel in the prey.
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC -!- PTM: The non-amidated UyCT3 does not show antimicrobial activity.
CC {ECO:0000269|PubMed:23182832}.
CC -!- MASS SPECTROMETRY: Mass=1433.5; Method=Electrospray; Note=Average
CC mass.; Evidence={ECO:0000269|PubMed:23182832};
CC -!- SIMILARITY: Belongs to the non-disulfide-bridged peptide (NDBP)
CC superfamily. Short antimicrobial peptide (group 4) family.
CC {ECO:0000305}.
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DR EMBL; JX274241; AGA82755.1; -; mRNA.
DR AlphaFoldDB; L0GCI6; -.
DR SMR; L0GCI6; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Amidation; Antibiotic; Antimicrobial; Cleavage on pair of basic residues;
KW Cytolysis; Hemolysis; Membrane; Secreted; Signal; Target cell membrane;
KW Target membrane; Transmembrane.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT PEPTIDE 24..36
FT /note="Antimicrobial peptide UyCT3"
FT /id="PRO_5001091941"
FT PROPEP 40..68
FT /evidence="ECO:0000255"
FT /id="PRO_5001091942"
FT MOD_RES 36
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:23182832"
SQ SEQUENCE 68 AA; 7830 MW; B95C97D28CB4978E CRC64;
MKNQFVLLLL AIVFLQMIFQ SDAILSAIWS GIKSLFGKRG LENMDKFDEL FDGDLSEADL
DFLKELMR
