ID   NDB4S_TITCO             Reviewed;          73 AA.
AC   Q5G8B5;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 25.
DE   RecName: Full=Putative antimicrobial peptide clone 4;
DE   AltName: Full=Non-disulfide-bridged peptide 4.23-like {ECO:0000250|UniProtKB:S6D3A7};
DE            Short=NDBP-4.23-like {ECO:0000250|UniProtKB:S6D3A7};
DE   Flags: Precursor;
OS   Tityus costatus (Brazilian scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Tityus.
OX   NCBI_TaxID=309814;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=15683865; DOI=10.1016/j.toxicon.2004.10.014;
RA   Diego-Garcia E., Batista C.V.F., Garcia-Gomez B.I., Lucas S., Candido D.M.,
RA   Gomez-Lagunas F., Possani L.D.;
RT   "The Brazilian scorpion Tityus costatus Karsch: genes, peptides and
RT   function.";
RL   Toxicon 45:273-283(2005).
RN   [2]
RP   FUNCTION, AND SYNTHESIS OF 23-39.
RX   PubMed=27917162; DOI=10.3389/fmicb.2016.01844;
RA   Guilhelmelli F., Vilela N., Smidt K.S., de Oliveira M.A.,
RA   da Cunha Morales Alvares A., Rigonatto M.C., da Silva Costa P.H.,
RA   Tavares A.H., de Freitas S.M., Nicola A.M., Franco O.L., Derengowski L.D.,
RA   Schwartz E.F., Mortari M.R., Bocca A.L., Albuquerque P., Silva-Pereira I.;
RT   "Activity of scorpion venom-derived antifungal peptides against planktonic
RT   cells of Candida spp. and Cryptococcus neoformans and Candida albicans
RT   biofilms.";
RL   Front. Microbiol. 7:1844-1844(2016).
CC   -!- FUNCTION: Antimicrobial peptide (PubMed:27917162) (By similarity). Has
CC       a high antibacterial activity against the Gram-positive bacterium
CC       S.aureus (MIC=5-17.30 uM), the methicillin-resistant S.aureus (MRSA)
CC       (MIC=17.30 uM), and E.faecalis (MIC=69.23 uM) (By similarity). Has
CC       antifungal activity against Candida spp. and one Cryptococcus
CC       neoformans strains with MICs values ranging from 6.25 to 100 uM
CC       (PubMed:27917162). Shows also an inhibitory activity on C.albicans
CC       biofilms at high concentrations (PubMed:27917162). Has a moderate
CC       hemolytic potency (18% at 20 uM) (By similarity). Also inhibits the
CC       growth of the five cancer cell lines tested (By similarity). In the
CC       model of polymicrobial sepsis, it exhibits an antibiotic effect,
CC       reducing the levels of microorganisms in the infectious focus and the
CC       inflammatory responses in the lung and cecum of septic animals (By
CC       similarity). {ECO:0000250|UniProtKB:S6D3A7,
CC       ECO:0000269|PubMed:27917162}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:15683865}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:15683865}.
CC   -!- MISCELLANEOUS: The primary structure of the mature peptide is identical
CC       to that of NDBP4.23 from Tityus serrulatus (AC S6D3A7). {ECO:0000305}.
CC   -!- MISCELLANEOUS: Does not show antifungal activity against Candida
CC       glabrata (ATCC90030) (MIC>400 uM). {ECO:0000269|PubMed:27917162}.
CC   -!- SIMILARITY: Belongs to the non-disulfide-bridged peptide (NDBP)
CC       superfamily. Short antimicrobial peptide (group 4) family.
CC       {ECO:0000305}.
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DR   EMBL; AY740686; AAW72456.1; -; mRNA.
DR   AlphaFoldDB; Q5G8B5; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Antibiotic; Antimicrobial; Cleavage on pair of basic residues; Fungicide;
KW   Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PEPTIDE         23..39
FT                   /note="Putative antimicrobial peptide clone 4"
FT                   /evidence="ECO:0000305|PubMed:15683865"
FT                   /id="PRO_0000231509"
FT   PROPEP          45..73
FT                   /evidence="ECO:0000305|PubMed:15683865"
FT                   /id="PRO_0000231510"
SQ   SEQUENCE   73 AA;  8418 MW;  93BAEE6D261D92BA CRC64;
     MQMKYLIPIF FLVLIVADHC HAFLGMIPGL IGGLISAFKG RRKRDITSQI EQYRNLQKRE
     AELEDILANL PVY