ID   NDB4T_OPICY             Reviewed;          69 AA.
AC   C5J887;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   03-AUG-2022, entry version 17.
DE   RecName: Full=Amphipathic peptide OcyC2 {ECO:0000303|PubMed:19379768};
DE   AltName: Full=Non-disulfide-bridged peptide 5.8 {ECO:0000303|PubMed:19379768};
DE            Short=NDBP-5.8 {ECO:0000303|PubMed:19379768};
DE   Contains:
DE     RecName: Full=OcyC2f {ECO:0000303|PubMed:19379768};
DE   Flags: Precursor;
OS   Opisthacanthus cayaporum (South American scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Iurida; Scorpionoidea; Hemiscorpiidae; Opisthacanthus.
OX   NCBI_TaxID=573324;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-35, MASS SPECTROMETRY,
RP   SUBCELLULAR LOCATION, AND NOMENCLATURE.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=19379768; DOI=10.1016/j.toxicon.2009.04.010;
RA   Silva E.C., Camargos T.S., Maranhao A.Q., Silva-Pereira I., Silva L.P.,
RA   Possani L.D., Schwartz E.F.;
RT   "Cloning and characterization of cDNA sequences encoding for new venom
RT   peptides of the Brazilian scorpion Opisthacanthus cayaporum.";
RL   Toxicon 54:252-261(2009).
RN   [2]
RP   FUNCTION, AND SYNTHESIS OF 27-37.
RX   PubMed=27917162; DOI=10.3389/fmicb.2016.01844;
RA   Guilhelmelli F., Vilela N., Smidt K.S., de Oliveira M.A.,
RA   da Cunha Morales Alvares A., Rigonatto M.C., da Silva Costa P.H.,
RA   Tavares A.H., de Freitas S.M., Nicola A.M., Franco O.L., Derengowski L.D.,
RA   Schwartz E.F., Mortari M.R., Bocca A.L., Albuquerque P., Silva-Pereira I.;
RT   "Activity of scorpion venom-derived antifungal peptides against planktonic
RT   cells of Candida spp. and Cryptococcus neoformans and Candida albicans
RT   biofilms.";
RL   Front. Microbiol. 7:1844-1844(2016).
CC   -!- FUNCTION: Amphipathic peptide with antimicrobial activity
CC       (PubMed:27917162). Shows antifungal activity with MIC values ranging
CC       from 25 to 200 uM (PubMed:27917162). {ECO:0000269|PubMed:27917162}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19379768}. Target
CC       cell membrane {ECO:0000250}. Note=Forms an alpha-helical membrane
CC       channel in the prey. {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:19379768}.
CC   -!- MASS SPECTROMETRY: [Amphipathic peptide OcyC2]: Mass=1511.91;
CC       Method=Unknown; Note=Monoisotopic mass.;
CC       Evidence={ECO:0000269|PubMed:19379768};
CC   -!- MASS SPECTROMETRY: [OcyC2f]: Mass=1286.84; Method=Unknown;
CC       Note=Monoisotopic mass.; Evidence={ECO:0000269|PubMed:19379768};
CC   -!- MISCELLANEOUS: Does not show antifungal activity against Candida
CC       glabrata (ATCC90030) and Candida parapsilosis (ATCC22019) (MIC>400 uM).
CC       {ECO:0000269|PubMed:27917162}.
CC   -!- SIMILARITY: Belongs to the non-disulfide-bridged peptide (NDBP)
CC       superfamily. Short antimicrobial peptide (group 4) family.
CC       {ECO:0000305}.
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DR   EMBL; FM998744; CAX51391.1; -; mRNA.
DR   AlphaFoldDB; C5J887; -.
DR   SMR; C5J887; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Antimicrobial; Direct protein sequencing; Fungicide; Membrane;
KW   Secreted; Signal; Target cell membrane; Target membrane.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000269|PubMed:19379768"
FT   PEPTIDE         24..37
FT                   /note="Amphipathic peptide OcyC2"
FT                   /id="PRO_5000471232"
FT   PEPTIDE         24..35
FT                   /note="OcyC2f"
FT                   /evidence="ECO:0000269|PubMed:19379768"
FT                   /id="PRO_5000471231"
FT   PROPEP          41..69
FT                   /id="PRO_0000398605"
FT   MOD_RES         37
FT                   /note="Isoleucine amide"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   69 AA;  7771 MW;  E9DE9D182191E2F5 CRC64;
     MKTQFAILMI AVVLMQMLVQ TEGGILGKIW EGVKSLIGKR GLKKLDQLDD TFDSDLSDAD
     VKLLREMFK