ID   NDB4_HETSP              Reviewed;          67 AA.
AC   A0A0C4G4L1;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2015, sequence version 1.
DT   03-AUG-2022, entry version 9.
DE   RecName: Full=Spiniferin {ECO:0000303|PubMed:24389272};
DE   Flags: Precursor;
OS   Heterometrus spinifer (Asia giant forest scorpion) (Malaysian black
OS   scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Iurida; Scorpionoidea; Scorpionidae; Heterometrinae;
OC   Heterometrus.
OX   NCBI_TaxID=118530;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, SYNTHESIS OF 24-36,
RP   MUTAGENESIS OF GLU-27 AND ASP-34, AND PROBABLE AMIDATION AT LEU-36.
RX   PubMed=24389272; DOI=10.1016/j.peptides.2013.12.012;
RA   Wu S., Nie Y., Zeng X.C., Cao H., Zhang L., Zhou L., Yang Y., Luo X.,
RA   Liu Y.;
RT   "Genomic and functional characterization of three new venom peptides from
RT   the scorpion Heterometrus spinifer.";
RL   Peptides 53:30-41(2014).
CC   -!- FUNCTION: Alpha-helical and amphipathic peptide with weak antimicrobial
CC       activities against both Gram-positive (MIC=41 uM to >82 uM) and Gram-
CC       negative (MIC>82 uM) bacteria. It has extremely weak hemolytic activity
CC       against human erythrocytes. {ECO:0000269|PubMed:24389272}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:24389272}. Target
CC       cell membrane {ECO:0000305|PubMed:24389272}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000250|UniProtKB:P0DME7}.
CC   -!- SIMILARITY: Belongs to the non-disulfide-bridged peptide (NDBP)
CC       superfamily. Short antimicrobial peptide (group 4) family.
CC       {ECO:0000305}.
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DR   EMBL; KC538871; AGK88597.1; -; mRNA.
DR   EMBL; KC538872; AGK88598.1; -; Genomic_DNA.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Antimicrobial; Cleavage on pair of basic residues; Membrane;
KW   Secreted; Signal; Target cell membrane; Target membrane.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   PEPTIDE         24..36
FT                   /note="Spiniferin"
FT                   /evidence="ECO:0000305|PubMed:24389272"
FT                   /id="PRO_5007393681"
FT   PROPEP          40..67
FT                   /evidence="ECO:0000305|PubMed:24389272"
FT                   /id="PRO_0000454585"
FT   MOD_RES         36
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000305|PubMed:24389272"
FT   MUTAGEN         27
FT                   /note="E->K: In spiniferin-M; increase in antibacterial and
FT                   hemolytic activity."
FT                   /evidence="ECO:0000269|PubMed:24389272"
FT   MUTAGEN         34
FT                   /note="D->N: In spiniferin-M; increase in antibacterial and
FT                   hemolytic activity."
FT                   /evidence="ECO:0000269|PubMed:24389272"
SQ   SEQUENCE   67 AA;  7600 MW;  32A3F83DA2274A57 CRC64;
     MKTQLAILLI TLVLFQMFSQ SDAILGEIWK GIKDILGKRG LNDLSDLDEL FDGEISKADL
     DFLREIM