ID   NDBH2_TITSE             Reviewed;          25 AA.
AC   P84190; P84192;
DT   13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   13-JUN-2006, sequence version 1.
DT   25-MAY-2022, entry version 32.
DE   RecName: Full=Hypotensin-2 {ECO:0000305|PubMed:18445483};
DE   AltName: Full=Hypotensin II {ECO:0000303|PubMed:18445483};
DE            Short=TsHpt-II {ECO:0000303|PubMed:18445483};
DE   AltName: Full=Toxin Ts14 2 {ECO:0000303|PubMed:19689419};
DE   Contains:
DE     RecName: Full=Hypotensin-4 {ECO:0000305|PubMed:18445483};
DE     AltName: Full=Hypotensin IV {ECO:0000303|PubMed:18445483};
DE              Short=TsHpt-IV {ECO:0000303|PubMed:18445483};
DE     AltName: Full=Toxin Ts14 4 {ECO:0000303|PubMed:19689419};
OS   Tityus serrulatus (Brazilian scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Tityus.
OX   NCBI_TaxID=6887;
RN   [1]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=18445483; DOI=10.1016/j.bbrc.2008.04.104;
RA   Verano-Braga T., Rocha-Resende C., Silva D.M., Ianzer D.,
RA   Martin-Eauclaire M.F., Bougis P.E., de Lima M.E., Santos R.A.S.,
RA   Pimenta A.M.C.;
RT   "Tityus serrulatus hypotensins: a new family of peptides from scorpion
RT   venom.";
RL   Biochem. Biophys. Res. Commun. 371:515-520(2008).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND PHOSPHORYLATION AT SER-6.
RX   PubMed=23731212; DOI=10.1021/pr4003068;
RA   Verano-Braga T., Dutra A.A., Leon I.R., Melo-Braga M.N., Roepstorff P.,
RA   Pimenta A.M., Kjeldsen F.;
RT   "Moving pieces in a venomic puzzle: unveiling post-translationally modified
RT   toxins from Tityus serrulatus.";
RL   J. Proteome Res. 12:3460-3470(2013).
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=19689419; DOI=10.2174/092986609788923329;
RA   Cologna C.T., Marcussi S., Giglio J.R., Soares A.M., Arantes E.C.;
RT   "Tityus serrulatus scorpion venom and toxins: an overview.";
RL   Protein Pept. Lett. 16:920-932(2009).
CC   -!- FUNCTION: Agonist of the B2 bradykinin receptor (BDKRB2). Potentiates
CC       the hypotensive effect of bradykinin (BK) and induces a direct
CC       vasorelaxing effect, independently of BK, by endothelium- and nitric
CC       oxide (NO)-dependent mechanisms in rat aortic ring preparations. Does
CC       not inhibit the angiotensin-converting enzyme (ACE). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18445483}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:18445483}.
CC   -!- PTM: The ultimate C-terminal Ala residue of TsHpt-I is quickly removed
CC       by carboxypeptidase Y in (in vitro) experiments. {ECO:0000250}.
CC   -!- MASS SPECTROMETRY: [Hypotensin-2]: Mass=2725.6; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:18445483};
CC   -!- MASS SPECTROMETRY: [Hypotensin-4]: Mass=2654.6; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:18445483};
CC   -!- SIMILARITY: Belongs to the non-disulfide-bridged peptide (NDBP)
CC       superfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P84190; -.
DR   iPTMnet; P84190; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0008217; P:regulation of blood pressure; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Bradykinin receptor impairing toxin; Direct protein sequencing;
KW   G-protein coupled receptor impairing toxin; Hypotensive agent;
KW   Phosphoprotein; Secreted; Toxin.
FT   PEPTIDE         1..25
FT                   /note="Hypotensin-2"
FT                   /evidence="ECO:0000269|PubMed:18445483"
FT                   /id="PRO_0000239434"
FT   PEPTIDE         1..24
FT                   /note="Hypotensin-4"
FT                   /evidence="ECO:0000269|PubMed:18445483"
FT                   /id="PRO_0000239436"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            23..24
FT                   /note="Important for potentiating BK effects"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         6
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:23731212"
SQ   SEQUENCE   25 AA;  2726 MW;  8D99F16FE445C0C6 CRC64;
     AEIDFSGIPE DIIKEIKETN AKPPA