NDC80_ARATH
ID NDC80_ARATH Reviewed; 568 AA.
AC Q9M1G5; A4FVQ9;
DT 23-MAY-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Kinetochore protein NDC80 homolog {ECO:0000303|PubMed:29356153};
GN Name=NDC80 {ECO:0000303|PubMed:29356153};
GN OrderedLocusNames=At3g54630 {ECO:0000312|Araport:AT3G54630};
GN ORFNames=T14E10_200 {ECO:0000312|EMBL:CAB77583.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=29356153; DOI=10.1111/tpj.13823;
RA Shin J., Jeong G., Park J.Y., Kim H., Lee I.;
RT "MUN (MERISTEM UNSTRUCTURED), encoding a SPC24 homolog of NDC80 kinetochore
RT complex, affects development through cell division in Arabidopsis
RT thaliana.";
RL Plant J. 93:977-991(2018).
CC -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC NDC80 complex, which is required for chromosome segregation and spindle
CC checkpoint activity to ensure proper cell division.
CC {ECO:0000305|PubMed:29356153}.
CC -!- SUBUNIT: Component of the NDC80 complex, which consists of NDC80, NUF2,
CC SPC24 and SPC25. {ECO:0000269|PubMed:29356153}.
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere
CC {ECO:0000269|PubMed:29356153}.
CC -!- SIMILARITY: Belongs to the NDC80/HEC1 family. {ECO:0000305}.
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DR EMBL; AL138656; CAB77583.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79259.1; -; Genomic_DNA.
DR EMBL; BT030357; ABO38770.1; -; mRNA.
DR PIR; T47622; T47622.
DR RefSeq; NP_191024.1; NM_115320.2.
DR AlphaFoldDB; Q9M1G5; -.
DR SMR; Q9M1G5; -.
DR STRING; 3702.AT3G54630.1; -.
DR PaxDb; Q9M1G5; -.
DR PRIDE; Q9M1G5; -.
DR ProteomicsDB; 251125; -.
DR EnsemblPlants; AT3G54630.1; AT3G54630.1; AT3G54630.
DR GeneID; 824628; -.
DR Gramene; AT3G54630.1; AT3G54630.1; AT3G54630.
DR KEGG; ath:AT3G54630; -.
DR Araport; AT3G54630; -.
DR TAIR; locus:2096996; AT3G54630.
DR eggNOG; KOG0995; Eukaryota.
DR HOGENOM; CLU_019866_0_0_1; -.
DR InParanoid; Q9M1G5; -.
DR OMA; IRFKVHV; -.
DR OrthoDB; 925552at2759; -.
DR PhylomeDB; Q9M1G5; -.
DR PRO; PR:Q9M1G5; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M1G5; differential.
DR GO; GO:0031262; C:Ndc80 complex; IEA:InterPro.
DR GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IEA:InterPro.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR Gene3D; 1.10.418.30; -; 1.
DR InterPro; IPR005550; Kinetochore_Ndc80.
DR InterPro; IPR038273; Ndc80_sf.
DR Pfam; PF03801; Ndc80_HEC; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Mitosis;
KW Reference proteome.
FT CHAIN 1..568
FT /note="Kinetochore protein NDC80 homolog"
FT /id="PRO_0000444102"
FT REGION 1..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 241..334
FT /evidence="ECO:0000255"
FT COILED 433..469
FT /evidence="ECO:0000255"
FT COMPBIAS 28..48
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 260
FT /note="T -> N (in Ref. 3; ABO38770)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 568 AA; 63332 MW; 92B151FD6D486995 CRC64;
MRGGAAGKRR TTVGFGGAPP PPPPSIEQQR HLFNSRDSDA SFASSRPSSI GLGGRGASDD
RSSMIRFINA FLSTHNFPIS IRGNPVPSVK DISETLKFLL SALDYPCDSI KWDEDLVFFL
KSQKCPFKIT KSSLKAPNTP HNWPTVLAVV HWLAELARFH QHLVSNSTSV PEDNSMNFFA
IQSFGHFIRG EDDKVNDLDS QFLGKLEAEK TSVAETISGC EKISGELEAK LESLRKGPSK
KESLEKVKAD LENDVNKFRT IVVEYTDRNP AMEKVVEEKA KELKAKEEER ERISVENKEL
KKSVELQNFS AADVNRMRRE LQAVERDVAD AEVARDGWDQ KAWELNSQIR NQFHQIQTLA
IDCNQALRRL KLDIQFAVNE RGETPAAVMG VDYKSVVKPA LCSLCDGIKG SSAEKVEELV
TLQHHKSEMA SKIESKRSLL GSIQLQINDL EEKMKLVKKE TQELSTKCDL EAKTLVESVK
AEALNLEVVE KEAAEFVKAS ELRLQEAVKE SEEEVQACAA QLFALIDSIS KQKEYMDSKI
SEIKTGVADT ASAVSEIYKA NFKKNLGI
