NDC80_CAEEL
ID NDC80_CAEEL Reviewed; 590 AA.
AC Q17635; Q23107;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Kinetochore protein ndc-80;
DE Short=CeNDC-80;
DE AltName: Full=Hec1 homolog;
GN Name=ndc-80; ORFNames=W01B6.9;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX
RP WITH HIM-10 AND KNL-1, AND SUBCELLULAR LOCATION.
RX PubMed=14522947; DOI=10.1101/gad.1126303;
RA Desai A., Rybina S., Mueller-Reichert T., Shevchenko A., Shevchenko A.,
RA Hyman A., Oegema K.;
RT "KNL-1 directs assembly of the microtubule-binding interface of the
RT kinetochore in C. elegans.";
RL Genes Dev. 17:2421-2435(2003).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=18765790; DOI=10.1101/gad.1687508;
RA Gassmann R., Essex A., Hu J.-S., Maddox P.S., Motegi F., Sugimoto A.,
RA O'Rourke S.M., Bowerman B., McLeod I., Yates J.R. III, Oegema K.,
RA Cheeseman I.M., Desai A.;
RT "A new mechanism controlling kinetochore-microtubule interactions revealed
RT by comparison of two dynein-targeting components: SPDL-1 and the
RT Rod/Zwilch/Zw10 complex.";
RL Genes Dev. 22:2385-2399(2008).
RN [4]
RP FUNCTION.
RX PubMed=19109417; DOI=10.1091/mbc.e08-10-1047;
RA Essex A., Dammermann A., Lewellyn L., Oegema K., Desai A.;
RT "Systematic analysis in Caenorhabditis elegans reveals that the spindle
RT checkpoint is composed of two largely independent branches.";
RL Mol. Biol. Cell 20:1252-1267(2009).
RN [5]
RP FUNCTION.
RX PubMed=23085020; DOI=10.1016/j.devcel.2012.09.012;
RA Schmidt J.C., Arthanari H., Boeszoermenyi A., Dashkevich N.M.,
RA Wilson-Kubalek E.M., Monnier N., Markus M., Oberer M., Milligan R.A.,
RA Bathe M., Wagner G., Grishchuk E.L., Cheeseman I.M.;
RT "The kinetochore-bound Ska1 complex tracks depolymerizing microtubules and
RT binds to curved protofilaments.";
RL Dev. Cell 23:968-980(2012).
RN [6]
RP FUNCTION, INTERACTION WITH ROD-1 AND ZWL-1, DISRUPTION PHENOTYPE, AND
RP MUTAGENESIS OF THR-8; SER-18; SER-44; SER-51; LYS-100; LYS-144 AND HIS-155.
RX PubMed=24231804; DOI=10.1126/science.1246232;
RA Cheerambathur D.K., Gassmann R., Cook B., Oegema K., Desai A.;
RT "Crosstalk between microtubule attachment complexes ensures accurate
RT chromosome segregation.";
RL Science 342:1239-1242(2013).
CC -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC ndc-80 complex, which is required for chromosome segregation in mitosis
CC and meiosis and spindle checkpoint activity (PubMed:14522947). Plays a
CC role in kinetochore assembly and recruits the checkpoint protein mdf-2
CC and the spindly-like protein spdl-1 to unattached kinetochores
CC (PubMed:18765790, PubMed:19109417, PubMed:24231804). Mediates the
CC formation of end-on kinetochore-microtubule attachments through
CC recruitment of spdl-1 (PubMed:24231804). The ndc-80 complex
CC synergistically enhances the affinity of the ska-1 complex for
CC microtubules and may allow the ndc-80 complex to track depolymerizing
CC microtubules (PubMed:23085020). {ECO:0000269|PubMed:14522947,
CC ECO:0000269|PubMed:18765790, ECO:0000269|PubMed:19109417,
CC ECO:0000269|PubMed:23085020, ECO:0000269|PubMed:24231804}.
CC -!- SUBUNIT: Component of the NDC80 complex, which is composed of at least
CC ndc-80 and him-10 (PubMed:14522947). The NDC80 complex interacts with
CC knl-1 (PubMed:14522947). Interacts with the RZZ complex components rod-
CC 1 (via N-terminus) and zwl-1 (PubMed:24231804).
CC {ECO:0000269|PubMed:14522947, ECO:0000269|PubMed:24231804}.
CC -!- INTERACTION:
CC Q17635; Q21952: him-10; NbExp=6; IntAct=EBI-314429, EBI-326288;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14522947}.
CC Chromosome, centromere, kinetochore {ECO:0000269|PubMed:14522947,
CC ECO:0000269|PubMed:18765790}. Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:24231804}. Note=Localizes to kinetochores
CC (PubMed:14522947). The NDC80 complex localizes to microtubules.
CC {ECO:0000269|PubMed:24231804}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in embryonic
CC lethality (PubMed:24231804). RNAi-mediated knockdown results in
CC defective cell division characterized by irregular chromosome
CC segregation, premature spindle pole separation and defective formation
CC of kinetochore-microtubule attachments (PubMed:24231804). In addition
CC there is reduced localization of the spindly-like protein spdl-1 to
CC kinetochores (PubMed:18765790). {ECO:0000269|PubMed:18765790,
CC ECO:0000269|PubMed:24231804}.
CC -!- SIMILARITY: Belongs to the NDC80/HEC1 family. {ECO:0000305}.
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DR EMBL; Z68301; CAA92629.1; -; Genomic_DNA.
DR EMBL; Z70718; CAA92629.1; JOINED; Genomic_DNA.
DR PIR; T18927; T18927.
DR RefSeq; NP_501830.1; NM_069429.4.
DR AlphaFoldDB; Q17635; -.
DR SMR; Q17635; -.
DR BioGRID; 42976; 28.
DR ComplexPortal; CPX-806; Ndc80 complex.
DR DIP; DIP-25850N; -.
DR IntAct; Q17635; 11.
DR STRING; 6239.W01B6.9; -.
DR EPD; Q17635; -.
DR PaxDb; Q17635; -.
DR PeptideAtlas; Q17635; -.
DR PRIDE; Q17635; -.
DR EnsemblMetazoa; W01B6.9.1; W01B6.9.1; WBGene00003576.
DR GeneID; 177873; -.
DR KEGG; cel:CELE_W01B6.9; -.
DR UCSC; W01B6.9.1; c. elegans.
DR CTD; 177873; -.
DR WormBase; W01B6.9; CE06537; WBGene00003576; ndc-80.
DR eggNOG; KOG0995; Eukaryota.
DR GeneTree; ENSGT00390000018386; -.
DR HOGENOM; CLU_454349_0_0_1; -.
DR InParanoid; Q17635; -.
DR OMA; IRFKVHV; -.
DR OrthoDB; 925552at2759; -.
DR PhylomeDB; Q17635; -.
DR SignaLink; Q17635; -.
DR PRO; PR:Q17635; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00003576; Expressed in adult organism and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000776; C:kinetochore; IDA:UniProtKB.
DR GO; GO:0005874; C:microtubule; IDA:ComplexPortal.
DR GO; GO:0031262; C:Ndc80 complex; IDA:WormBase.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0019904; F:protein domain specific binding; IPI:WormBase.
DR GO; GO:0044877; F:protein-containing complex binding; IPI:UniProtKB.
DR GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IMP:WormBase.
DR GO; GO:0008608; P:attachment of spindle microtubules to kinetochore; IDA:ComplexPortal.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0035262; P:gonad morphogenesis; IMP:WormBase.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0007079; P:mitotic chromosome movement towards spindle pole; IGI:UniProtKB.
DR GO; GO:1905561; P:positive regulation of kinetochore assembly; IGI:UniProtKB.
DR GO; GO:1901970; P:positive regulation of mitotic sister chromatid separation; IGI:UniProtKB.
DR GO; GO:1905342; P:positive regulation of protein localization to kinetochore; IMP:UniProtKB.
DR GO; GO:0034501; P:protein localization to kinetochore; IMP:UniProtKB.
DR GO; GO:1903394; P:protein localization to kinetochore involved in kinetochore assembly; IMP:UniProtKB.
DR GO; GO:0030334; P:regulation of cell migration; IMP:WormBase.
DR Gene3D; 1.10.418.30; -; 1.
DR InterPro; IPR005550; Kinetochore_Ndc80.
DR InterPro; IPR038273; Ndc80_sf.
DR PANTHER; PTHR10643; PTHR10643; 1.
DR Pfam; PF03801; Ndc80_HEC; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Cytoplasm;
KW Cytoskeleton; Kinetochore; Meiosis; Mitosis; Nucleus; Reference proteome.
FT CHAIN 1..590
FT /note="Kinetochore protein ndc-80"
FT /id="PRO_0000249557"
FT COILED 269..342
FT /evidence="ECO:0000255"
FT COILED 450..525
FT /evidence="ECO:0000255"
FT MUTAGEN 8
FT /note="T->A: Rescues embryonic lethality and chromosome
FT segregation defects in single knockdown mutant; when
FT associated with A-18, A-44 and A-51."
FT /evidence="ECO:0000269|PubMed:24231804"
FT MUTAGEN 18
FT /note="S->A: Rescues embryonic lethality and chromosome
FT segregation defects in single knockdown mutant; when
FT associated with A-8, A-44 and A-51."
FT /evidence="ECO:0000269|PubMed:24231804"
FT MUTAGEN 44
FT /note="S->A: Rescues embryonic lethality and chromosome
FT segregation defects in single knockdown mutant; when
FT associated with A-8, A-18 and A-51."
FT /evidence="ECO:0000269|PubMed:24231804"
FT MUTAGEN 51
FT /note="S->A: Rescues embryonic lethality and chromosome
FT segregation defects in single knockdown mutant; when
FT associated with A-8, A-18 and A-44."
FT /evidence="ECO:0000269|PubMed:24231804"
FT MUTAGEN 100
FT /note="K->A: Embryonic lethal and inhibits NDC80 complex
FT binding to microtubules; when associated with A-144 and A-
FT 155."
FT /evidence="ECO:0000269|PubMed:24231804"
FT MUTAGEN 144
FT /note="K->A: Embryonic lethal and inhibits NDC80 complex
FT binding to microtubules; when associated with A-100 and A-
FT 155."
FT /evidence="ECO:0000269|PubMed:24231804"
FT MUTAGEN 155
FT /note="H->A: Embryonic lethal and inhibits NDC80 complex
FT binding to microtubules; when associated with A-100 and A-
FT 144."
FT /evidence="ECO:0000269|PubMed:24231804"
SQ SEQUENCE 590 AA; 68913 MW; 77548C2EE701E8CD CRC64;
MFGDRRKTGG LNLNGRASIA ITPTKRFTDY TGSTSVRKTD ARPSLSQPRV SLFNTKNSSV
APRDVKSLVS LNGSKIYNFL VEYESSDAPS EQLIMKPRGK NDFIACFELI YQHLSKDYEF
PRHERIEEEV SQIFKGLGYP YPLKNSYYQP MGSSHGYPHL LDALSWLIDI IRINSAVSED
TQNILFGDFM EQGKAQEKTL NYAWMTSTFR DYTNDRKAAE NPSSSYWDDT KHRLRKYFEQ
SNEFEDMTKT AASALEMLNY ECDEIEADKG NEASLKEEIS RIRDDIRKAK DYLEQNLHVK
QHMEKELAMV KSEQEEKISE NEKVQKMVDD LKNKIELQKQ IHGLTGKEVR QMNLDNNKDK
EVVLEIQSEL DRLSKETWKL KDEDFFKEQK SKFIHLAEQI MKILSGLNIQ MNLEPLRAPT
NERDLKDYWE TLNKIWVPEI SRQLHQRKLE LETEQSRFSN KAVTAEERIQ IQSETLCEAK
KNEAREERIR RNERDSWKDA RKHIEQRYEQ LLNEKEVLLK QMKLDGSLEK EIEDETARMS
ATGEEHIQKR SQLEAGIRQI LDLMVVEIAE IENKKIGFHV QCAGIEKAVL
