ID   NDC80_DANRE             Reviewed;         632 AA.
AC   Q6DRJ7; Q502U1;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Kinetochore protein NDC80 homolog;
DE   AltName: Full=Kinetochore protein Hec1;
DE   AltName: Full=Kinetochore-associated protein 2-like;
GN   Name=ndc80; Synonyms=kntc2, kntc2l; ORFNames=zgc:111801;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RX   PubMed=15256591; DOI=10.1073/pnas.0403929101;
RA   Amsterdam A., Nissen R.M., Sun Z., Swindell E.C., Farrington S.,
RA   Hopkins N.;
RT   "Identification of 315 genes essential for early zebrafish development.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:12792-12797(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Liver;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       NDC80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity. Required for kinetochore integrity and the
CC       organization of stable microtubule binding sites in the outer plate of
CC       the kinetochore. The NDC80 complex synergistically enhances the
CC       affinity of the SKA1 complex for microtubules and may allow the NDC80
CC       complex to track depolymerizing microtubules. May play a role in
CC       chromosome congression and may be essential for the end-on attachment
CC       of the kinetochores to spindle microtubules.
CC       {ECO:0000250|UniProtKB:O14777}.
CC   -!- SUBUNIT: Component of the NDC80 complex, which is composed of ndc80,
CC       cdca1, spbc24 and spbc25. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8AWF5}.
CC       Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q8AWF5}.
CC       Note=Localizes to kinetochores from late prophase to anaphase.
CC       {ECO:0000250|UniProtKB:Q8AWF5}.
CC   -!- SIMILARITY: Belongs to the NDC80/HEC1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH95554.1; Type=Miscellaneous discrepancy; Note=Artifact. Missing internal sequence that doesn't correspond to an exon-intron boundary.; Evidence={ECO:0000305};
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DR   EMBL; AY648762; AAT68080.1; -; mRNA.
DR   EMBL; BC095554; AAH95554.1; ALT_SEQ; mRNA.
DR   RefSeq; NP_001003863.1; NM_001003863.1.
DR   AlphaFoldDB; Q6DRJ7; -.
DR   SMR; Q6DRJ7; -.
DR   STRING; 7955.ENSDARP00000012236; -.
DR   PaxDb; Q6DRJ7; -.
DR   Ensembl; ENSDART00000007335; ENSDARP00000012236; ENSDARG00000071694.
DR   GeneID; 445386; -.
DR   KEGG; dre:445386; -.
DR   CTD; 10403; -.
DR   ZFIN; ZDB-GENE-030131-904; ndc80.
DR   eggNOG; KOG0995; Eukaryota.
DR   GeneTree; ENSGT00390000018386; -.
DR   HOGENOM; CLU_012583_2_0_1; -.
DR   InParanoid; Q6DRJ7; -.
DR   OMA; WVAERAQ; -.
DR   OrthoDB; 925552at2759; -.
DR   PhylomeDB; Q6DRJ7; -.
DR   TreeFam; TF101177; -.
DR   Reactome; R-DRE-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR   Reactome; R-DRE-2467813; Separation of Sister Chromatids.
DR   Reactome; R-DRE-2500257; Resolution of Sister Chromatid Cohesion.
DR   Reactome; R-DRE-5663220; RHO GTPases Activate Formins.
DR   Reactome; R-DRE-9648025; EML4 and NUDC in mitotic spindle formation.
DR   PRO; PR:Q6DRJ7; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 12.
DR   Bgee; ENSDARG00000071694; Expressed in testis and 29 other tissues.
DR   ExpressionAtlas; Q6DRJ7; baseline.
DR   GO; GO:0000776; C:kinetochore; ISS:UniProtKB.
DR   GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0140483; F:kinetochore adaptor activity; ISS:UniProtKB.
DR   GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; ISS:UniProtKB.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; ISS:UniProtKB.
DR   GO; GO:0051310; P:metaphase plate congression; ISS:UniProtKB.
DR   GO; GO:0007052; P:mitotic spindle organization; ISS:UniProtKB.
DR   GO; GO:0090267; P:positive regulation of mitotic cell cycle spindle assembly checkpoint; ISS:UniProtKB.
DR   Gene3D; 1.10.418.30; -; 1.
DR   InterPro; IPR040967; DUF5595.
DR   InterPro; IPR005550; Kinetochore_Ndc80.
DR   InterPro; IPR038273; Ndc80_sf.
DR   PANTHER; PTHR10643; PTHR10643; 1.
DR   Pfam; PF18077; DUF5595; 1.
DR   Pfam; PF03801; Ndc80_HEC; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..632
FT                   /note="Kinetochore protein NDC80 homolog"
FT                   /id="PRO_0000249554"
FT   COILED          263..419
FT                   /evidence="ECO:0000255"
FT   COILED          459..632
FT                   /evidence="ECO:0000255"
FT   CONFLICT        162
FT                   /note="L -> H (in Ref. 2; AAH95554)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        392
FT                   /note="N -> R (in Ref. 2; AAH95554)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        618
FT                   /note="L -> R (in Ref. 2; AAH95554)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   632 AA;  73217 MW;  9FBA099115E4D8BF CRC64;
     MSRRPSSRYS EMPMRVTDSR MSLINATPQN KDNAFGKLNI PKPQSTTSER RTSFFGKGIG
     AGGQRNSMFG SYGGSEKMKD PRALHDKAFV QQCIKQLYEF LVDRGFPGSI TVKALQSPST
     KEFLKIYEFI YNFLEPSFQM PTAKVEEEIP RMLKDLGYPF ALSKSSMYSI GAPHTWPLAL
     GALIWLMDAV KLFGGQREQD LLFSDFSDEL CDLEDRTEYN KLIMEYCSDT YNKFMQGADT
     FDDEDDDFLY KLKKLYNVDE ALLHSQQEKH SMLMEHVERL ERESQTDRLV GKRTEKLRLQ
     TDLQKLQNYR CTLEAHKTGL ENKSAGLTEE LEAVEMQLEG LKQERTRLQH ILENQKFTPA
     DIERINRERN ELQQTIHGLS QSLEEGEQLV WNEEVNLSKT KEKAELKVAE YNKLGRKLKL
     IPLSAENACG HDFEIRADYS ATTITQYKTQ IQNPLKNMMV EVEEEFSRLS NVNLSLEETV
     EQVKSNIFDK ENDIKQLKEQ IRKVDQQLEN AMQEMALEDD KWAAELDSAE THKKLFEKNV
     MQGIEEAEEE VKAAQQQYHV VVQETNEKNR MVVKNMTDLF SSTVNHLFAV EKHCDEQLKR
     FDKLKDIVRE DEADINQLTD LVENFIKKAN SL