ID   NDC80_XENLA             Reviewed;         638 AA.
AC   Q8AWF5; Q6NRM2;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   19-SEP-2006, sequence version 2.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Kinetochore protein NDC80 homolog;
DE   AltName: Full=Kinetochore protein Hec1;
DE   AltName: Full=Kinetochore-associated protein 2;
DE            Short=xNdc80;
GN   Name=ndc80; Synonyms=kntc2;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CDCA1, AND
RP   SUBCELLULAR LOCATION.
RX   PubMed=12514103; DOI=10.1101/gad.1040903;
RA   McCleland M.L., Gardner R.D., Kallio M.J., Daum J.R., Gorbsky G.J.,
RA   Burke D.J., Stukenberg P.T.;
RT   "The highly conserved Ndc80 complex is required for kinetochore assembly,
RT   chromosome congression, and spindle checkpoint activity.";
RL   Genes Dev. 17:101-114(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Oocyte;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE
RP   NDC80 COMPLEX.
RX   PubMed=14738735; DOI=10.1016/j.cub.2003.12.058;
RA   McCleland M.L., Kallio M.J., Barrett-Wilt G.A., Kestner C.A.,
RA   Shabanowitz J., Hunt D.F., Gorbsky G.J., Stukenberg P.T.;
RT   "The vertebrate Ndc80 complex contains Spc24 and Spc25 homologs, which are
RT   required to establish and maintain kinetochore-microtubule attachment.";
RL   Curr. Biol. 14:131-137(2004).
RN   [4]
RP   CHARACTERIZATION OF THE NDC80 COMPLEX, AND INTERACTION OF THE NDC80 COMPLEX
RP   WITH MIS12 AND ZWINT.
RX   PubMed=16079178; DOI=10.1091/mbc.e05-03-0239;
RA   Emanuele M.J., McCleland M.L., Satinover D.L., Stukenberg P.T.;
RT   "Measuring the stoichiometry and physical interactions between components
RT   elucidates the architecture of the vertebrate kinetochore.";
RL   Mol. Biol. Cell 16:4882-4892(2005).
RN   [5]
RP   INTERACTION WITH CEP57R.
RX   PubMed=17803911; DOI=10.1016/j.cell.2007.07.023;
RA   Emanuele M.J., Stukenberg P.T.;
RT   "Xenopus Cep57 is a novel kinetochore component involved in microtubule
RT   attachment.";
RL   Cell 130:893-905(2007).
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       NDC80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity (PubMed:12514103, PubMed:14738735). Required for
CC       kinetochore integrity and the organization of stable microtubule
CC       binding sites in the outer plate of the kinetochore. The NDC80 complex
CC       synergistically enhances the affinity of the SKA1 complex for
CC       microtubules and may allow the NDC80 complex to track depolymerizing
CC       microtubules. May play a role in chromosome congression and may be
CC       essential for the end-on attachment of the kinetochores to spindle
CC       microtubules (By similarity). {ECO:0000250|UniProtKB:O14777,
CC       ECO:0000269|PubMed:12514103, ECO:0000269|PubMed:14738735}.
CC   -!- SUBUNIT: Component of the NDC80 complex, which is composed of ndc80,
CC       cdca1, spbc24 and spbc25. The NDC80 complex interacts with mis12 and
CC       zwint. Interacts with cep57r. {ECO:0000269|PubMed:12514103,
CC       ECO:0000269|PubMed:14738735, ECO:0000269|PubMed:16079178,
CC       ECO:0000269|PubMed:17803911}.
CC   -!- INTERACTION:
CC       Q8AWF5; Q8AWF4: nuf2-a; NbExp=8; IntAct=EBI-1000337, EBI-1000342;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12514103}.
CC       Chromosome, centromere, kinetochore {ECO:0000269|PubMed:12514103}.
CC       Note=Localizes to kinetochores from late prophase to anaphase.
CC       {ECO:0000269|PubMed:12514103}.
CC   -!- SIMILARITY: Belongs to the NDC80/HEC1 family. {ECO:0000305}.
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DR   EMBL; AY168013; AAN87031.1; -; mRNA.
DR   EMBL; BC070725; AAH70725.1; -; mRNA.
DR   RefSeq; NP_001082372.1; NM_001088903.1.
DR   RefSeq; XP_018103626.1; XM_018248137.1.
DR   AlphaFoldDB; Q8AWF5; -.
DR   SMR; Q8AWF5; -.
DR   IntAct; Q8AWF5; 6.
DR   DNASU; 398429; -.
DR   GeneID; 398429; -.
DR   KEGG; xla:398429; -.
DR   CTD; 398429; -.
DR   Xenbase; XB-GENE-997357; ndc80.S.
DR   OMA; WVAERAQ; -.
DR   OrthoDB; 925552at2759; -.
DR   Proteomes; UP000186698; Chromosome 2S.
DR   Bgee; 398429; Expressed in egg cell and 19 other tissues.
DR   GO; GO:0000776; C:kinetochore; ISS:UniProtKB.
DR   GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0140483; F:kinetochore adaptor activity; ISS:UniProtKB.
DR   GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; ISS:UniProtKB.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; ISS:UniProtKB.
DR   GO; GO:0051310; P:metaphase plate congression; ISS:UniProtKB.
DR   GO; GO:0007052; P:mitotic spindle organization; ISS:UniProtKB.
DR   GO; GO:0090267; P:positive regulation of mitotic cell cycle spindle assembly checkpoint; ISS:UniProtKB.
DR   Gene3D; 1.10.418.30; -; 1.
DR   InterPro; IPR040967; DUF5595.
DR   InterPro; IPR005550; Kinetochore_Ndc80.
DR   InterPro; IPR038273; Ndc80_sf.
DR   PANTHER; PTHR10643; PTHR10643; 1.
DR   Pfam; PF18077; DUF5595; 1.
DR   Pfam; PF03801; Ndc80_HEC; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..638
FT                   /note="Kinetochore protein NDC80 homolog"
FT                   /id="PRO_0000249555"
FT   REGION          1..55
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          249..394
FT                   /evidence="ECO:0000255"
FT   COILED          479..638
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        1..15
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        22..55
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        345
FT                   /note="Q -> R (in Ref. 1; AAN87031)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   638 AA;  74477 MW;  6D725844D425F29E CRC64;
     MRRSSVTNRQ SLLPLRVQDA NRMGLTTPQN KDRQGFGKLS MSKPHSGTSE KKTSFFGKRA
     SNGTARTSQY GAFGGTEKIK DPRPLHDKAF IQQCIRQLCE FLNENGYSQA LTVKSLQGPS
     TKDFLKIFAF IYTFICPNYE NPESKFEEEI PRIFKELGYP FALSKSSMYT VGAPHTWPQI
     VAALVWLIDC VKLCCVLRSE NPVFEDPPMG EQSENGIDFN QLFLDYTVRC YDQFMEGRDT
     FEEYDTDVCI RLKEAYHVDE SNLEALQQES RRLMEEIERL EKEKENEPDR LASMRKLKAS
     LQADIQKYQN YLTEIESHST LLDQRVSSIS EELEATELES RAIQQENLRL KNILDNQKYS
     VADIERIKYE ETELQQTIAK LTKDLDEDKQ QLWHEELKYA KMKESVETQL SEFHKIGRKV
     RLIPPTAEFA NGYDFQIQCN LDSEQSSLMH YRNKINVPLV EILSQSEGHI ASSTNKKMGV
     EDMVEQLNTL IGEKKNEVKV QKDEAQKLEE IYQQKVEESE EHEKRWISEI ESLEKHRQLL
     ESGVNKSLDE AMKDLQKEQQ ELQLVEHRTE EEMRQVENKL VRVVHAVANH VAVIEKHLEE
     KRLKVEREYE EFMKEDLLLD LRELLEKYKE KARVLDSL