ID   NDC80_XENTR             Reviewed;         640 AA.
AC   Q5U4X5;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Kinetochore protein NDC80 homolog;
DE   AltName: Full=Kinetochore protein Hec1;
DE   AltName: Full=Kinetochore-associated protein 2;
GN   Name=ndc80; Synonyms=kntc2;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       NDC80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity. Required for kinetochore integrity and the
CC       organization of stable microtubule binding sites in the outer plate of
CC       the kinetochore. The NDC80 complex synergistically enhances the
CC       affinity of the SKA1 complex for microtubules and may allow the NDC80
CC       complex to track depolymerizing microtubules. May play a role in
CC       chromosome congression and may be essential for the end-on attachment
CC       of the kinetochores to spindle microtubules.
CC       {ECO:0000250|UniProtKB:O14777, ECO:0000250|UniProtKB:Q8AWF5}.
CC   -!- SUBUNIT: Component of the NDC80 complex, which is composed of ndc80,
CC       cdca1, spbc24 and spbc25. The NDC80 complex interacts with mis12 and
CC       zwint. Interacts with cep57r (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8AWF5}.
CC       Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q8AWF5}.
CC       Note=Localizes to kinetochores from late prophase to anaphase.
CC       {ECO:0000250|UniProtKB:Q8AWF5}.
CC   -!- SIMILARITY: Belongs to the NDC80/HEC1 family. {ECO:0000305}.
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DR   EMBL; BC084918; AAH84918.1; -; mRNA.
DR   RefSeq; NP_001011162.1; NM_001011162.1.
DR   RefSeq; XP_012809877.1; XM_012954423.2.
DR   AlphaFoldDB; Q5U4X5; -.
DR   SMR; Q5U4X5; -.
DR   STRING; 8364.ENSXETP00000045005; -.
DR   DNASU; 496580; -.
DR   Ensembl; ENSXETT00000045005; ENSXETP00000045005; ENSXETG00000020840.
DR   GeneID; 496580; -.
DR   KEGG; xtr:496580; -.
DR   CTD; 10403; -.
DR   Xenbase; XB-GENE-997352; ndc80.
DR   eggNOG; KOG0995; Eukaryota.
DR   InParanoid; Q5U4X5; -.
DR   OrthoDB; 925552at2759; -.
DR   Reactome; R-XTR-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR   Reactome; R-XTR-2467813; Separation of Sister Chromatids.
DR   Reactome; R-XTR-2500257; Resolution of Sister Chromatid Cohesion.
DR   Reactome; R-XTR-5663220; RHO GTPases Activate Formins.
DR   Reactome; R-XTR-9648025; EML4 and NUDC in mitotic spindle formation.
DR   Proteomes; UP000008143; Chromosome 2.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000020840; Expressed in 2-cell stage embryo and 15 other tissues.
DR   ExpressionAtlas; Q5U4X5; baseline.
DR   GO; GO:0000776; C:kinetochore; ISS:UniProtKB.
DR   GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0140483; F:kinetochore adaptor activity; ISS:UniProtKB.
DR   GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; ISS:UniProtKB.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; ISS:UniProtKB.
DR   GO; GO:0051310; P:metaphase plate congression; ISS:UniProtKB.
DR   GO; GO:0007052; P:mitotic spindle organization; ISS:UniProtKB.
DR   GO; GO:0090267; P:positive regulation of mitotic cell cycle spindle assembly checkpoint; ISS:UniProtKB.
DR   Gene3D; 1.10.418.30; -; 1.
DR   InterPro; IPR040967; DUF5595.
DR   InterPro; IPR005550; Kinetochore_Ndc80.
DR   InterPro; IPR038273; Ndc80_sf.
DR   PANTHER; PTHR10643; PTHR10643; 1.
DR   Pfam; PF18077; DUF5595; 1.
DR   Pfam; PF03801; Ndc80_HEC; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..640
FT                   /note="Kinetochore protein NDC80 homolog"
FT                   /id="PRO_0000249556"
FT   REGION          1..79
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          249..394
FT                   /evidence="ECO:0000255"
FT   COILED          467..636
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        1..15
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        22..71
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   640 AA;  74637 MW;  C21F7CF074F48ED6 CRC64;
     MRRSSVTNRQ SLLPLRVQDA NRMGLTTPQS KDRQGFGKLS MSKPHSGTSE RKTSFFGKRM
     SNGTARTSQY GAFGGTEKIK DPRPLHDKAF IQQCIRQLCE FLNENGYSQT LTVKSLQGPS
     TKDFLKIFAF IYTFICPNYE NPESKFEEEI PRIFKELGYP FALSKSSMYT VGAPHTWPQI
     VAALVWLIDC VKLCCVLRSE NPVFEEPQMG EQSENGIDFN QLFLDYTVRC YDQFMEGRDT
     FEEYDADMCI RLKEAYHVDE SNLEALQQES RRLLEEIERL EKEKENEPDR LASMRKLKVS
     LQADIQKYQN YLTEIESHST LLEQRVSSVS EDLEATELES RAVQQENLRL KNILDNQKYS
     VADIERIKYE ENELQQTIAK LTKDLDEDKQ QLWSEELKYA KMKESVETQL SEFHKIGRKV
     RLIPPTAEYA NGYDFQIQCN LDSEQSSLIH YRNKINVPLV EILSQSEGQI AGATNKKMGV
     EDMVEQLNTL IGEKKNEVKV QKDEAQKLEE TYQQKVEEAE EDEKRWISEI ESLEKHRQLL
     ESGVNKSLDE ALKDLQKEQQ ELQLVEHQTE EEMRQVENKL VRVVHAVANH VAVIEKHLEE
     KRLKVEREYE EFMKEDLLLD LRELLDKYKE KARVLDSPPY