ID   NDE1A_XENLA             Reviewed;         349 AA.
AC   Q66J96; O13025;
DT   13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=Nuclear distribution protein nudE homolog 1-A;
DE   AltName: Full=Mitotic phosphoprotein 43;
DE            Short=MP43;
GN   Name=nde1-a;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PHOSPHORYLATION.
RX   PubMed=9115395; DOI=10.1016/s0960-9822(06)00157-6;
RA   Stukenberg P.T., Lustig K.D., McGarry T.J., King R.W., Kuang J.,
RA   Kirschner M.W.;
RT   "Systematic identification of mitotic phosphoproteins.";
RL   Curr. Biol. 7:338-348(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=11163258; DOI=10.1016/s0896-6273(00)00145-8;
RA   Feng Y., Olson E.C., Stukenberg P.T., Flanagan L.A., Kirschner M.W.,
RA   Walsh C.A.;
RT   "LIS1 regulates CNS lamination by interacting with mNudE, a central
RT   component of the centrosome.";
RL   Neuron 28:665-679(2000).
CC   -!- FUNCTION: Required for centrosome duplication and formation and
CC       function of the mitotic spindle. {ECO:0000250}.
CC   -!- SUBUNIT: Self-associates. Interacts with pafah1b1 (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}. Cytoplasm,
CC       cytoskeleton, microtubule organizing center, centrosome {ECO:0000250}.
CC       Cytoplasm, cytoskeleton, spindle {ECO:0000250}. Note=Localizes to the
CC       interphase centrosome and to the mitotic spindle. {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Strongly expressed in the eye, branchial arches
CC       and telencephalon of stage 26-33 embryos.
CC       {ECO:0000269|PubMed:11163258}.
CC   -!- PTM: Phosphorylated in mitosis. {ECO:0000269|PubMed:9115395}.
CC   -!- SIMILARITY: Belongs to the nudE family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC60121.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; U95097; AAC60121.1; ALT_INIT; mRNA.
DR   EMBL; BC081010; AAH81010.1; -; mRNA.
DR   RefSeq; NP_001081814.1; NM_001088345.1.
DR   AlphaFoldDB; Q66J96; -.
DR   SMR; Q66J96; -.
DR   IntAct; Q66J96; 2.
DR   DNASU; 398067; -.
DR   GeneID; 398067; -.
DR   KEGG; xla:398067; -.
DR   CTD; 398067; -.
DR   Xenbase; XB-GENE-6252032; nde1.L.
DR   OMA; EQTVNRR; -.
DR   OrthoDB; 1082224at2759; -.
DR   Proteomes; UP000186698; Chromosome 9_10L.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0031616; C:spindle pole centrosome; ISS:UniProtKB.
DR   GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0051298; P:centrosome duplication; ISS:UniProtKB.
DR   InterPro; IPR033494; NUDE.
DR   InterPro; IPR006964; NUDE_dom.
DR   PANTHER; PTHR10921; PTHR10921; 1.
DR   Pfam; PF04880; NUDE_C; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton;
KW   Microtubule; Mitosis; Phosphoprotein; Reference proteome.
FT   CHAIN           1..349
FT                   /note="Nuclear distribution protein nudE homolog 1-A"
FT                   /id="PRO_0000240206"
FT   COILED          22..189
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   349 AA;  39711 MW;  B9F0CEE49EDB98A1 CRC64;
     MDDLENNIFN SVEEEILYWK SVAMKYKQCS EEAQQELQEF QEASREYEAE LEAQLLQTEG
     RNRDLFSENN RLRMELDAIK EKYEEQHSEN YVQISTLEGD LSQTKAVRDQ LQKYIRELEQ
     ANDDLERAKR ATIMSLEDFE QRLNQAIERN AFLENELDEK ENLLESVQRL KDEARDLRQE
     LAVQQKQEKP KSNMVSPETE RMDTSVQASI AIPSAPLTPL SQRGCASTLT SPLSFRTSLD
     DGYSGTPLTP CARISALNIV GDLLRKVGAL ESKLASCRNF VHEQSPNRPL TSVSARMKKT
     REGIENRLSI ASGSSVEKGL IKRLEFGSFP SNTPMQGMHS PQGVVKMII