NDE1_DROME
ID NDE1_DROME Reviewed; 317 AA.
AC Q9VT70; Q95RM2;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Nuclear distribution protein nudE homolog {ECO:0000303|PubMed:26490864};
GN Name=nudE {ECO:0000303|PubMed:26490864, ECO:0000312|FlyBase:FBgn0036059};
GN ORFNames=CG8104 {ECO:0000312|FlyBase:FBgn0036059};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26490864; DOI=10.1523/jneurosci.1610-15.2015;
RA Wang X., Zhang M.W., Kim J.H., Macara A.M., Sterne G., Yang T., Ye B.;
RT "The Krueppel-Like factor Dar1 determines multipolar neuron morphology.";
RL J. Neurosci. 35:14251-14259(2015).
CC -!- FUNCTION: Chaperone protein with functions in nuclear localization
CC (PubMed:26490864). Required for centrosome duplication and formation
CC and function of the mitotic spindle (By similarity). In postmitotic
CC neurons, acts with nudC downstream of dar1 to ensure correct
CC positioning of the nuclei in primary dendrites and as a consequence, is
CC required for determining multipolar neuron morphology
CC (PubMed:26490864). {ECO:0000250|UniProtKB:Q9NXR1,
CC ECO:0000269|PubMed:26490864}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}. Cytoplasm,
CC cytoskeleton, microtubule organizing center, centrosome {ECO:0000250}.
CC Cytoplasm, cytoskeleton, spindle {ECO:0000250}. Note=Localizes to the
CC interphase centrosome and to the mitotic spindle. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in multipolar
CC Class I dendritic arborizing neurons forming a bipolar morphology
CC (PubMed:26490864). Dendritic arborization is unaffected
CC (PubMed:26490864). {ECO:0000269|PubMed:26490864}.
CC -!- SIMILARITY: Belongs to the nudE family. {ECO:0000305}.
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DR EMBL; AE014296; AAF50184.2; -; Genomic_DNA.
DR EMBL; AY061280; AAL28828.1; -; mRNA.
DR RefSeq; NP_648373.1; NM_140116.3.
DR AlphaFoldDB; Q9VT70; -.
DR SMR; Q9VT70; -.
DR BioGRID; 64556; 17.
DR IntAct; Q9VT70; 1.
DR STRING; 7227.FBpp0112072; -.
DR PaxDb; Q9VT70; -.
DR PRIDE; Q9VT70; -.
DR DNASU; 39169; -.
DR EnsemblMetazoa; FBtr0076344; FBpp0076073; FBgn0036059.
DR GeneID; 39169; -.
DR KEGG; dme:Dmel_CG8104; -.
DR UCSC; CG8104-RA; d. melanogaster.
DR CTD; 39169; -.
DR FlyBase; FBgn0036059; nudE.
DR VEuPathDB; VectorBase:FBgn0036059; -.
DR eggNOG; KOG1853; Eukaryota.
DR GeneTree; ENSGT00390000000111; -.
DR InParanoid; Q9VT70; -.
DR BioGRID-ORCS; 39169; 0 hits in 3 CRISPR screens.
DR ChiTaRS; nudE; fly.
DR GenomeRNAi; 39169; -.
DR PRO; PR:Q9VT70; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0036059; Expressed in egg cell and 21 other tissues.
DR ExpressionAtlas; Q9VT70; baseline and differential.
DR Genevisible; Q9VT70; DM.
DR GO; GO:0030424; C:axon; IDA:FlyBase.
DR GO; GO:0005813; C:centrosome; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0030425; C:dendrite; IDA:FlyBase.
DR GO; GO:0005871; C:kinesin complex; IBA:GO_Central.
DR GO; GO:0000776; C:kinetochore; IDA:FlyBase.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005635; C:nuclear envelope; IDA:FlyBase.
DR GO; GO:0005819; C:spindle; IDA:FlyBase.
DR GO; GO:0070732; C:spindle envelope; IDA:FlyBase.
DR GO; GO:0031616; C:spindle pole centrosome; ISS:UniProtKB.
DR GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR GO; GO:0007409; P:axonogenesis; IMP:FlyBase.
DR GO; GO:0007298; P:border follicle cell migration; IMP:FlyBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0016477; P:cell migration; IBA:GO_Central.
DR GO; GO:0051298; P:centrosome duplication; ISS:UniProtKB.
DR GO; GO:0051642; P:centrosome localization; IMP:FlyBase.
DR GO; GO:0007059; P:chromosome segregation; IBA:GO_Central.
DR GO; GO:0048813; P:dendrite morphogenesis; IMP:FlyBase.
DR GO; GO:0051303; P:establishment of chromosome localization; IBA:GO_Central.
DR GO; GO:0000132; P:establishment of mitotic spindle orientation; IBA:GO_Central.
DR GO; GO:0030951; P:establishment or maintenance of microtubule cytoskeleton polarity; IMP:FlyBase.
DR GO; GO:0007294; P:germarium-derived oocyte fate determination; IMP:FlyBase.
DR GO; GO:0007060; P:male meiosis chromosome segregation; IMP:FlyBase.
DR GO; GO:0007020; P:microtubule nucleation; IBA:GO_Central.
DR GO; GO:0007100; P:mitotic centrosome separation; IBA:GO_Central.
DR GO; GO:0007080; P:mitotic metaphase plate congression; IMP:FlyBase.
DR GO; GO:0007052; P:mitotic spindle organization; IMP:FlyBase.
DR GO; GO:0051647; P:nucleus localization; IMP:FlyBase.
DR GO; GO:0050775; P:positive regulation of dendrite morphogenesis; IGI:FlyBase.
DR GO; GO:0047496; P:vesicle transport along microtubule; IBA:GO_Central.
DR InterPro; IPR033494; NUDE.
DR InterPro; IPR006964; NUDE_dom.
DR PANTHER; PTHR10921; PTHR10921; 1.
DR Pfam; PF04880; NUDE_C; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton;
KW Microtubule; Mitosis; Reference proteome.
FT CHAIN 1..317
FT /note="Nuclear distribution protein nudE homolog"
FT /id="PRO_0000240209"
FT REGION 186..205
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 29..180
FT /evidence="ECO:0000255"
SQ SEQUENCE 317 AA; 36239 MW; FBDECA1685AF983D CRC64;
MESPPMFNSV EDECRYWKER SKQYHKEWTD VKQEYDEFVE QSREMEIEMD ATLDQKQSII
KDLTAKLTMF ERENESLKLK LESHGIDMSN MEKQLETVKK DRDTMKVYLR QLEQKNDDLE
RAHRILNESI ENFEKMLDQA YEKNALLELE VDEKGLLQEK LQRLMDETRD LKQELNVKSR
FTPVVNGTSV PTANDTNTVN SSMNSSASLP NGIVANGELV KHDNAVATRA TSVSVNALNG
SLVNRNEYNQ QHSLKSDGDE TEDVPREWSA NAAATPLLPA IHTSGAGRTA LLLAGCRVVW
ILQFSVIHKP QIRNPIT
