NDF1_DANRE
ID NDF1_DANRE Reviewed; 350 AA.
AC O42202;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Neurogenic differentiation factor 1;
DE Short=NeuroD;
DE Short=NeuroD1;
GN Name=neurod1 {ECO:0000250|UniProtKB:Q13562};
GN Synonyms=neurod {ECO:0000312|ZFIN:ZDB-GENE-990415-172},
GN nrd {ECO:0000312|EMBL:AAB70529.1};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAB70529.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=9409673; DOI=10.1242/dev.124.22.4557;
RA Blader P., Fischer N., Gradwohl G., Guillemont F., Straehle U.;
RT "The activity of neurogenin1 is controlled by local cues in the zebrafish
RT embryo.";
RL Development 124:4557-4569(1997).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAB88820.1}
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo {ECO:0000269|PubMed:9733104};
RX PubMed=9733104;
RX DOI=10.1002/(sici)1097-0177(199809)213:1<92::aid-aja9>3.0.co;2-t;
RA Korzh V., Sleptsova I., Liao J., He J., Gong Z.;
RT "Expression of zebrafish bHLH genes ngn1 and nrd defines distinct stages of
RT neural differentiation.";
RL Dev. Dyn. 213:92-104(1998).
RN [3] {ECO:0000312|EMBL:AAH56603.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo {ECO:0000312|EMBL:AAH56603.1};
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX PubMed=10235116; DOI=10.1089/104454999315394;
RA Liao J., He J., Yan T., Korzh V., Gong Z.;
RT "A class of neuroD-related basic helix-loop-helix transcription factors
RT expressed in developing central nervous system in zebrafish.";
RL DNA Cell Biol. 18:333-344(1999).
RN [5] {ECO:0000305}
RP FUNCTION, AND MUTAGENESIS OF ASN-107.
RX PubMed=12044886; DOI=10.1016/s0014-5793(02)02809-0;
RA Wang X., Korzh V., Gong Z.;
RT "The functional specificity of NeuroD is defined by a single amino acid
RT residue (N11) in the basic domain.";
RL FEBS Lett. 520:139-144(2002).
RN [6] {ECO:0000305}
RP TISSUE SPECIFICITY.
RX PubMed=12524185; DOI=10.1016/s0165-3806(02)00583-7;
RA Mueller T., Wullimann M.F.;
RT "Anatomy of neurogenesis in the early zebrafish brain.";
RL Brain Res. Dev. Brain Res. 140:137-155(2003).
CC -!- FUNCTION: May act as a transcriptional activator. Differentiation
CC factor required for neurogenesis. Acts as an upstream activator of
CC isl1. {ECO:0000269|PubMed:12044886}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. {ECO:0000250|UniProtKB:Q60867}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q64289}. Nucleus
CC {ECO:0000250|UniProtKB:Q64289, ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: In the embryo, expressed broadly in a subset of
CC primary neurons in the brain and spinal cord. At 28 hours post-
CC fertilization (hpf), regions of expression include telencephalon,
CC olfactory placode, epiphysis, cranial ganglia, acoustic ganglia, Rohon-
CC Beard mechano-sensory neurons and motoneurons. In 2 day postembryonic
CC brain, expressed in many brain regions but absent from subpallium, the
CC ventral preoptic region, ventral thalamus and hypothalamus; sites of
CC expression extend laterally from the ventricular proliferative regions
CC and correspond to freshly determined cell populations. In adult,
CC expressed in all tissues examined with highest levels in brain.
CC {ECO:0000269|PubMed:10235116, ECO:0000269|PubMed:12524185,
CC ECO:0000269|PubMed:9409673, ECO:0000269|PubMed:9733104}.
CC -!- DEVELOPMENTAL STAGE: First detected at 10 hpf (1-somite stage), with
CC increased levels during subsequent embryonic development. Also
CC expressed in adult. {ECO:0000269|PubMed:10235116,
CC ECO:0000269|PubMed:9409673, ECO:0000269|PubMed:9733104}.
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DR EMBL; AF017302; AAB70529.1; -; Genomic_DNA.
DR EMBL; AF036148; AAB88820.1; -; mRNA.
DR EMBL; BC056603; AAH56603.1; -; mRNA.
DR RefSeq; NP_571053.1; NM_130978.2.
DR AlphaFoldDB; O42202; -.
DR SMR; O42202; -.
DR STRING; 7955.ENSDARP00000026490; -.
DR PaxDb; O42202; -.
DR Ensembl; ENSDART00000011837; ENSDARP00000026490; ENSDARG00000019566.
DR GeneID; 30169; -.
DR KEGG; dre:30169; -.
DR CTD; 4760; -.
DR ZFIN; ZDB-GENE-990415-172; neurod1.
DR eggNOG; KOG3898; Eukaryota.
DR GeneTree; ENSGT00940000160478; -.
DR InParanoid; O42202; -.
DR OMA; FKHEPAA; -.
DR OrthoDB; 1096531at2759; -.
DR PhylomeDB; O42202; -.
DR TreeFam; TF315153; -.
DR PRO; PR:O42202; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 9.
DR Bgee; ENSDARG00000019566; Expressed in ganglion and 94 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0062139; P:camera-type eye photoreceptor cell development; IMP:ZFIN.
DR GO; GO:0031018; P:endocrine pancreas development; IMP:ZFIN.
DR GO; GO:0060119; P:inner ear receptor cell development; IMP:ZFIN.
DR GO; GO:0045746; P:negative regulation of Notch signaling pathway; IMP:ZFIN.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0048923; P:posterior lateral line neuromast hair cell differentiation; IMP:ZFIN.
DR GO; GO:0031099; P:regeneration; IMP:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR032652; Neurod1.
DR InterPro; IPR022575; Neurogenic_DUF.
DR InterPro; IPR016637; TF_bHLH_NeuroD.
DR PANTHER; PTHR19290:SF88; PTHR19290:SF88; 1.
DR Pfam; PF00010; HLH; 1.
DR Pfam; PF12533; Neuro_bHLH; 1.
DR PIRSF; PIRSF015618; bHLH_NeuroD; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; Developmental protein; Differentiation; DNA-binding;
KW Neurogenesis; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..350
FT /note="Neurogenic differentiation factor 1"
FT /id="PRO_0000274817"
FT DOMAIN 96..148
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..91
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 82..88
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 1..24
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..41
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 42..71
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 107
FT /note="N->S: Reduction in ability to induce ectopic
FT expression of isl1."
FT /evidence="ECO:0000269|PubMed:12044886"
SQ SEQUENCE 350 AA; 39415 MW; 90A3812107858433 CRC64;
MTKSYSEESM MLESQSSSNW TDKCHSSSQD ERDVDKTSEP MLNDMEDDDD AGLNRLEDED
DEEEEEEEED GDDTKPKRRG PKKKKMTKAR MQRFKMRRMK ANARERNRMH GLNDALESLR
KVVPCYSKTQ KLSKIETLRL AKNYIWALSE ILRSGKSPDL MSFVQALCKG LSQPTTNLVA
GCLQLNPRTF LPEQSQEMPP HMQTASASFS ALPYSYQTPG LPSPPYGTMD SSHIFHVKPH
AYGSALEPFF DTTLTDCTSP SFDGPLSPPL SVNGNFSFKH EPSSEFEKNY AFTMHYQAAG
LAGAQGHAAS LYAGSTQRCD IPMENIMSYD GHSHHERVMN AQLNAIFHDS
