ID   NDF4_CHICK              Reviewed;         330 AA.
AC   P79766;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Neurogenic differentiation factor 4;
DE            Short=NeuroD4;
DE   AltName: Full=NeuroM;
GN   Name=NEUROD4;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC   STRAIN=White leghorn; TISSUE=Embryonic retina;
RX   PubMed=9310321; DOI=10.1242/dev.124.17.3263;
RA   Roztocil T., Matter-Sadzinski L., Alliod C., Ballivet M., Matter J.-M.;
RT   "NeuroM, a neural helix-loop-helix transcription factor, defines a new
RT   transition stage in neurogenesis.";
RL   Development 124:3263-3272(1997).
CC   -!- FUNCTION: Probably acts as a transcriptional activator. Mediates
CC       neuronal differentiation. Required for the regulation of amacrine cell
CC       fate specification in the retina (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC       protein. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC   -!- TISSUE SPECIFICITY: Expressed in both the developing central nervous
CC       system and peripheral nervous system. {ECO:0000269|PubMed:9310321}.
CC   -!- DEVELOPMENTAL STAGE: Expression in the developing nervous system is
CC       transient and restricted to cells lining the ventricular zone that have
CC       ceased proliferation but have not yet begun to migrate into the outer
CC       layers. In retina, neurom is also transiently expressed in cells as
CC       they withdraw from the mitotic cycle, but persists in horizontal and
CC       bipolar neurons until full differentiation. In the peripheral nervous
CC       system, its expression closely follows cell proliferation.
CC       {ECO:0000269|PubMed:9310321}.
CC   -!- PTM: Serine or threonine phosphorylation within the basic region may
CC       regulate neurogenic activity. {ECO:0000250}.
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DR   EMBL; Y09597; CAA70785.1; -; mRNA.
DR   RefSeq; NP_990407.1; NM_205076.1.
DR   AlphaFoldDB; P79766; -.
DR   SMR; P79766; -.
DR   STRING; 9031.ENSGALP00000040481; -.
DR   PaxDb; P79766; -.
DR   GeneID; 395959; -.
DR   KEGG; gga:395959; -.
DR   CTD; 58158; -.
DR   VEuPathDB; HostDB:geneid_395959; -.
DR   eggNOG; KOG3898; Eukaryota.
DR   InParanoid; P79766; -.
DR   OrthoDB; 1096531at2759; -.
DR   PhylomeDB; P79766; -.
DR   PRO; PR:P79766; -.
DR   Proteomes; UP000000539; Unplaced.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0035881; P:amacrine cell differentiation; ISS:UniProtKB.
DR   GO; GO:0048666; P:neuron development; IEA:InterPro.
DR   GO; GO:0045597; P:positive regulation of cell differentiation; ISS:UniProtKB.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   InterPro; IPR032651; Neurod4.
DR   InterPro; IPR022575; Neurogenic_DUF.
DR   InterPro; IPR016637; TF_bHLH_NeuroD.
DR   PANTHER; PTHR19290:SF86; PTHR19290:SF86; 1.
DR   Pfam; PF00010; HLH; 1.
DR   Pfam; PF12533; Neuro_bHLH; 1.
DR   PIRSF; PIRSF015618; bHLH_NeuroD; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   2: Evidence at transcript level;
KW   Activator; Developmental protein; Differentiation; DNA-binding;
KW   Neurogenesis; Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..330
FT                   /note="Neurogenic differentiation factor 4"
FT                   /id="PRO_0000127395"
FT   DOMAIN          87..139
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          1..79
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          162..183
FT                   /note="Leucine-zipper"
FT   MOTIF           73..79
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   330 AA;  36707 MW;  96492F06A3C07B41 CRC64;
     MTKTYTKAKE MAELVGTQGW MDDALSSKDE LKAENGRPGP FGLVAGLNEE HDSIEEEEEE
     EDDGEKPKRR GPKKKKMTKA RLERFRARRV KANARERTRM HGLNDALDNL RRVMPCYSKT
     QKLSKIETLR LARNYIWALS EVLETGQTPE GKSFVEMLCK GLSQPTSNLV AGCLQLGPQT
     LFLEKHEEKT PGCESAISSH SFTYQSPGLP SPPYGSMETH LLHLKPPAFK SLVDASFGNP
     PDCTTPPYEG PLTPPLSISG NFSLKQDGSP DLDKPYAFMA HYPSVSLAGA HGHPTHFQNA
     VPRYEIPIDM SYESYPHHVA GPQLNAIFNE