NDF4_MOUSE
ID NDF4_MOUSE Reviewed; 330 AA.
AC O09105;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Neurogenic differentiation factor 4;
DE Short=NeuroD4;
DE AltName: Full=Helix-loop-helix protein mATH-3;
DE Short=mATH3;
DE AltName: Full=Protein atonal homolog 3;
GN Name=Neurod4; Synonyms=Ath3, Atoh3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Retina;
RX PubMed=9029157; DOI=10.1093/emboj/16.2.384;
RA Takebayashi K., Takahashi S., Yokota C., Tsuda H., Nakanishi S.,
RA Asashima M., Kageyama R.;
RT "Conversion of ectoderm into a neural fate by ATH-3, a vertebrate basic
RT helix-loop-helix gene homologous to Drosophila proneural gene atonal.";
RL EMBO J. 16:384-395(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=129/J;
RX PubMed=9497361; DOI=10.1074/jbc.273.11.6327;
RA Tsuda H., Takebayashi K., Nakanishi S., Kageyama R.;
RT "Structure and promoter analysis of Math3 gene, a mouse homolog of
RT Drosophila proneural gene atonal. Neural-specific expression by dual
RT promoter elements.";
RL J. Biol. Chem. 273:6327-6333(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Retina;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11861467; DOI=10.1242/dev.129.4.831;
RA Inoue T., Hojo M., Bessho Y., Tano Y., Lee J.E., Kageyama R.;
RT "Math3 and NeuroD regulate amacrine cell fate specification in the
RT retina.";
RL Development 129:831-842(2002).
CC -!- FUNCTION: Probably acts as a transcriptional activator. Mediates
CC neuronal differentiation. Required for the regulation of amacrine cell
CC fate specification in the retina. {ECO:0000269|PubMed:11861467}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Expressed in retinal interneurons amacrine cells
CC (at protein level). Expressed in neurons of ventricular zone of the
CC retina at postnatal day 1 (P1). Expressed transiently in amacrine and
CC horizontal cells of the inner nuclear layer (INL) of the retina at P7
CC until P14. {ECO:0000269|PubMed:11861467, ECO:0000269|PubMed:9029157,
CC ECO:0000269|PubMed:9497361}.
CC -!- DEVELOPMENTAL STAGE: Weakly expressed in the neural tube around 8.5
CC dpc. By 9.5 dpc, expression is prominent in the ventral brain and
CC spinal cord, and weak expression begins in the retina. At 10.5 dpc,
CC highly expressed in the trigeminal and dorsal root ganglions, and the
CC ventral midbrain and hindbrain. At 12.5 dpc, expressed in the mantle
CC layer of the brain and spinal cord, trigeminal ganglion, Rathke's
CC pouch, dorsal root ganglion and the ventricular zone of the neural
CC retina. At 16.5 dpc, expression becomes restricted to the anterior
CC region, persisting in the diencephalon and neural retina but decreasing
CC in other regions. {ECO:0000269|PubMed:9029157,
CC ECO:0000269|PubMed:9497361}.
CC -!- PTM: Serine or threonine phosphorylation within the basic region may
CC regulate neurogenic activity. {ECO:0000250}.
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DR EMBL; D85845; BAA12880.1; -; mRNA.
DR EMBL; AF036257; AAC15969.1; -; Genomic_DNA.
DR EMBL; BC054391; AAH54391.1; -; mRNA.
DR CCDS; CCDS24353.1; -.
DR RefSeq; NP_001316418.1; NM_001329489.1.
DR RefSeq; NP_001316419.1; NM_001329490.1.
DR RefSeq; NP_031527.1; NM_007501.5.
DR AlphaFoldDB; O09105; -.
DR BioGRID; 198239; 1.
DR IntAct; O09105; 1.
DR STRING; 10090.ENSMUSP00000051379; -.
DR PhosphoSitePlus; O09105; -.
DR PaxDb; O09105; -.
DR PRIDE; O09105; -.
DR Antibodypedia; 15429; 79 antibodies from 22 providers.
DR DNASU; 11923; -.
DR Ensembl; ENSMUST00000061571; ENSMUSP00000051379; ENSMUSG00000048015.
DR GeneID; 11923; -.
DR KEGG; mmu:11923; -.
DR UCSC; uc007hri.2; mouse.
DR CTD; 58158; -.
DR MGI; MGI:108055; Neurod4.
DR VEuPathDB; HostDB:ENSMUSG00000048015; -.
DR eggNOG; KOG3898; Eukaryota.
DR GeneTree; ENSGT00940000160217; -.
DR HOGENOM; CLU_055134_0_0_1; -.
DR InParanoid; O09105; -.
DR OMA; MAHYPSV; -.
DR OrthoDB; 1096531at2759; -.
DR PhylomeDB; O09105; -.
DR TreeFam; TF315153; -.
DR BioGRID-ORCS; 11923; 2 hits in 73 CRISPR screens.
DR PRO; PR:O09105; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; O09105; protein.
DR Bgee; ENSMUSG00000048015; Expressed in pineal body and 87 other tissues.
DR ExpressionAtlas; O09105; baseline and differential.
DR Genevisible; O09105; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0035881; P:amacrine cell differentiation; IDA:UniProtKB.
DR GO; GO:0043010; P:camera-type eye development; IGI:MGI.
DR GO; GO:0045165; P:cell fate commitment; IGI:MGI.
DR GO; GO:0097475; P:motor neuron migration; IMP:MGI.
DR GO; GO:0007405; P:neuroblast proliferation; IGI:MGI.
DR GO; GO:0048666; P:neuron development; IMP:MGI.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0007219; P:Notch signaling pathway; IGI:MGI.
DR GO; GO:0048709; P:oligodendrocyte differentiation; IGI:MGI.
DR GO; GO:0045597; P:positive regulation of cell differentiation; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR032651; Neurod4.
DR InterPro; IPR022575; Neurogenic_DUF.
DR InterPro; IPR016637; TF_bHLH_NeuroD.
DR PANTHER; PTHR19290:SF86; PTHR19290:SF86; 1.
DR Pfam; PF00010; HLH; 1.
DR Pfam; PF12533; Neuro_bHLH; 1.
DR PIRSF; PIRSF015618; bHLH_NeuroD; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; Differentiation; DNA-binding;
KW Neurogenesis; Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..330
FT /note="Neurogenic differentiation factor 4"
FT /id="PRO_0000127391"
FT DOMAIN 87..139
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 23..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 330 AA; 37133 MW; F8EC228F4EE0FF88 CRC64;
MAKMYMKSKD MVELVNTQSW MDKGLSSQNE MKEQERRPGS YGMLGTLTEE HDSIEEDEEE
EEDGDKPKRR GPKKKKMTKA RLERFRARRV KANARERTRM HGLNDALDNL RRVMPCYSKT
QKLSKIETLR LARNYIWALS EVLETGQTLE GKGFVEMLCK GLSQPTSNLV AGCLQLGPQS
TLLEKHEEKS SICDSTISVH SFNYQSPGLP SPPYGHMETH SLHLKPQPFK SLGDSFGSHP
PDCSTPPYEG PLTPPLSISG NFSLKQDGSP DLEKSYNFMP HYTSASLSSG HVHSTPFQTG
TPRYDVPVDL SYDSYSHHSI GTQLNTIFSD
