NDF6B_DANRE
ID NDF6B_DANRE Reviewed; 317 AA.
AC Q9W6C7; Q6DHB0;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 3.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Neurogenic differentiation factor 6-B;
DE Short=NeuroD6-B;
DE AltName: Full=Protein atonal homolog 2-B;
GN Name=neurod6b {ECO:0000250|UniProtKB:Q96NK8};
GN Synonyms=atoh2b {ECO:0000312|ZFIN:ZDB-GENE-010608-2},
GN ndr1b {ECO:0000312|EMBL:AAD23442.2}; ORFNames=zgc:92543;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAD23442.2}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE, AND TISSUE
RP SPECIFICITY.
RC TISSUE=Embryo {ECO:0000269|PubMed:10235116};
RX PubMed=10235116; DOI=10.1089/104454999315394;
RA Liao J., He J., Yan T., Korzh V., Gong Z.;
RT "A class of neuroD-related basic helix-loop-helix transcription factors
RT expressed in developing central nervous system in zebrafish.";
RL DNA Cell Biol. 18:333-344(1999).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAD23442.2}
RP SEQUENCE REVISION TO 85 AND 88, FUNCTION, AND MUTAGENESIS OF GLN-81 AND
RP SER-89.
RX PubMed=12044886; DOI=10.1016/s0014-5793(02)02809-0;
RA Wang X., Korzh V., Gong Z.;
RT "The functional specificity of NeuroD is defined by a single amino acid
RT residue (N11) in the basic domain.";
RL FEBS Lett. 520:139-144(2002).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAH76065.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain {ECO:0000312|EMBL:AAH76065.1};
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Differentiation factor required for neurogenesis (By
CC similarity). Does not act as an upstream activator of isl1.
CC {ECO:0000250|UniProtKB:Q6NYU3, ECO:0000269|PubMed:12044886}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. {ECO:0000250|UniProtKB:P48986}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1 {ECO:0000269|PubMed:10235116};
CC IsoId=Q9W6C7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9W6C7-2; Sequence=VSP_052291;
CC -!- TISSUE SPECIFICITY: Embryonic olfactory bulbs and olfactory placodes.
CC In adult, expressed in brain and eye. {ECO:0000269|PubMed:10235116}.
CC -!- DEVELOPMENTAL STAGE: Embryonic, larval and adult stages. First detected
CC at 22 hours post-fertilization (hpf). {ECO:0000269|PubMed:10235116}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD23442.2; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAH76065.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF115773; AAD23442.2; ALT_FRAME; mRNA.
DR EMBL; BC076065; AAH76065.1; ALT_INIT; mRNA.
DR RefSeq; NP_001296773.1; NM_001309844.1. [Q9W6C7-1]
DR RefSeq; NP_571892.3; NM_131817.3. [Q9W6C7-1]
DR AlphaFoldDB; Q9W6C7; -.
DR SMR; Q9W6C7; -.
DR STRING; 7955.ENSDARP00000088468; -.
DR PaxDb; Q9W6C7; -.
DR Ensembl; ENSDART00000018150; ENSDARP00000088468; ENSDARG00000020794. [Q9W6C7-1]
DR Ensembl; ENSDART00000111135; ENSDARP00000101953; ENSDARG00000020794. [Q9W6C7-1]
DR GeneID; 114415; -.
DR KEGG; dre:114415; -.
DR CTD; 114415; -.
DR ZFIN; ZDB-GENE-010608-2; neurod6b.
DR eggNOG; KOG3898; Eukaryota.
DR GeneTree; ENSGT00940000159827; -.
DR HOGENOM; CLU_055134_1_0_1; -.
DR InParanoid; Q9W6C7; -.
DR OMA; ANYPRES; -.
DR OrthoDB; 1096531at2759; -.
DR PhylomeDB; Q9W6C7; -.
DR TreeFam; TF315153; -.
DR PRO; PR:Q9W6C7; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 2.
DR Bgee; ENSDARG00000020794; Expressed in brain and 24 other tissues.
DR ExpressionAtlas; Q9W6C7; baseline.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR032650; Neurod6.
DR InterPro; IPR022575; Neurogenic_DUF.
DR PANTHER; PTHR19290:SF9; PTHR19290:SF9; 1.
DR Pfam; PF00010; HLH; 1.
DR Pfam; PF12533; Neuro_bHLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; Differentiation; DNA-binding;
KW Neurogenesis; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..317
FT /note="Neurogenic differentiation factor 6-B"
FT /id="PRO_0000274816"
FT DOMAIN 78..130
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..90
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 297..317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 65..70
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 69..90
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 299..317
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 92..167
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_052291"
FT MUTAGEN 81
FT /note="Q->M: Does not confer ability to induce ectopic
FT expression of isl1."
FT /evidence="ECO:0000269|PubMed:12044886"
FT MUTAGEN 89
FT /note="S->N: Confers ability to induce ectopic expression
FT of isl1."
FT /evidence="ECO:0000269|PubMed:12044886"
FT CONFLICT 247
FT /note="P -> N (in Ref. 1; AAD23442)"
FT /evidence="ECO:0000305"
FT CONFLICT 255
FT /note="F -> L (in Ref. 1; AAD23442)"
FT /evidence="ECO:0000305"
FT CONFLICT 273
FT /note="G -> E (in Ref. 2; AAH76065)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 317 AA; 36196 MW; A9D4823231DD2B68 CRC64;
MLTVPFEEPD MMRESQFGAT FTRQEDVRTL SSAELKEAED DNTDREEEEE REEDENGLPK
KKGPRKKKSE GRGDRVKMRR QEANARERSR MHGLNDALES LRKVVPCYSK TQKLSKIETL
RLAKNYIWAL SETLSAGKRP DLLAFVQTLC KGLSQPTTNL VAGCLQLNAR NFLTDHNGDV
SFSGRPAYDS LYPYPNAEMA TPTGLSSGTR ESVKPFRPYN YYASYESYYD SASPESSSPH
FDGQMSPPIN YNGIFSLKKH DEQVEYSKNC HYGMRYCNVP GRGSMYRVSP DSHFPYDLHP
RSQSFQSQDE LNTGYHN
