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NDHA_MYCTU
ID   NDHA_MYCTU              Reviewed;         470 AA.
AC   P95200; F2GN78; I6WYA2; Q7D9W6;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 147.
DE   RecName: Full=Type II NADH:quinone oxidoreductase NdhA {ECO:0000305};
DE            EC=1.6.5.9 {ECO:0000269|PubMed:15767566};
DE   AltName: Full=Type II NADH dehydrogenase NdhA {ECO:0000303|PubMed:25128581};
GN   Name=ndhA {ECO:0000303|PubMed:15767566};
GN   OrderedLocusNames=Rv0392c {ECO:0000312|EMBL:CCP43122.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, AND SUBCELLULAR
RP   LOCATION.
RC   STRAIN=H37Rv;
RX   PubMed=15767566; DOI=10.1073/pnas.0500469102;
RA   Weinstein E.A., Yano T., Li L.S., Avarbock D., Avarbock A., Helm D.,
RA   McColm A.A., Duncan K., Lonsdale J.T., Rubin H.;
RT   "Inhibitors of type II NADH:menaquinone oxidoreductase represent a class of
RT   antitubercular drugs.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:4548-4553(2005).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [4]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RX   PubMed=25128581; DOI=10.1016/j.gene.2014.08.024;
RA   Awasthy D., Ambady A., Narayana A., Morayya S., Sharma U.;
RT   "Roles of the two type II NADH dehydrogenases in the survival of
RT   Mycobacterium tuberculosis in vitro.";
RL   Gene 550:110-116(2014).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=29382761; DOI=10.1073/pnas.1721545115;
RA   Vilcheze C., Weinrick B., Leung L.W., Jacobs W.R. Jr.;
RT   "Plasticity of Mycobacterium tuberculosis NADH dehydrogenases and their
RT   role in virulence.";
RL   Proc. Natl. Acad. Sci. U.S.A. 115:1599-1604(2018).
CC   -!- FUNCTION: Alternative, nonproton pumping NADH:quinone oxidoreductase
CC       that delivers electrons to the respiratory chain by oxidation of NADH
CC       and reduction of quinones. {ECO:0000269|PubMed:15767566,
CC       ECO:0000269|PubMed:29382761}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a quinone + H(+) + NADH = a quinol + NAD(+);
CC         Xref=Rhea:RHEA:46160, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124; EC=1.6.5.9;
CC         Evidence={ECO:0000269|PubMed:15767566};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a menaquinone + H(+) + NADH = a menaquinol + NAD(+);
CC         Xref=Rhea:RHEA:29235, Rhea:RHEA-COMP:9537, Rhea:RHEA-COMP:9539,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16374, ChEBI:CHEBI:18151,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC         Evidence={ECO:0000269|PubMed:15767566};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000250|UniProtKB:Q2FZV7};
CC       Note=Binds 1 FAD per subunit. {ECO:0000250|UniProtKB:Q2FZV7};
CC   -!- ACTIVITY REGULATION: Inhibited by phenothiazine analogs.
CC       {ECO:0000269|PubMed:15767566}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:15767566}; Single-pass membrane protein
CC       {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the gene does not affect virulence in
CC       mice (PubMed:29382761). Deletion of the gene does not induce any major
CC       redox perturbation in M.tuberculosis (PubMed:29382761). Non-essential,
CC       can be deleted without causing any adverse effects in vitro
CC       (PubMed:25128581). The ndh-ndhA double knockout could not be obtained,
CC       suggesting that at least one type II NADH dehydrogenase is required for
CC       M.tuberculosis growth (PubMed:29382761). {ECO:0000269|PubMed:25128581,
CC       ECO:0000269|PubMed:29382761}.
CC   -!- SIMILARITY: Belongs to the NADH dehydrogenase family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP43122.1; -; Genomic_DNA.
DR   RefSeq; NP_214906.1; NC_000962.3.
DR   RefSeq; WP_003898428.1; NZ_NVQJ01000002.1.
DR   AlphaFoldDB; P95200; -.
DR   SMR; P95200; -.
DR   STRING; 83332.Rv0392c; -.
DR   PaxDb; P95200; -.
DR   PRIDE; P95200; -.
DR   DNASU; 886430; -.
DR   GeneID; 45424357; -.
DR   GeneID; 886430; -.
DR   KEGG; mtu:Rv0392c; -.
DR   PATRIC; fig|83332.111.peg.431; -.
DR   TubercuList; Rv0392c; -.
DR   eggNOG; COG1252; Bacteria.
DR   OMA; DHCIFLD; -.
DR   PhylomeDB; P95200; -.
DR   PHI-base; PHI:3629; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005829; C:cytosol; HDA:MTBBASE.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0003955; F:NAD(P)H dehydrogenase (quinone) activity; IDA:MTBBASE.
DR   GO; GO:0003954; F:NADH dehydrogenase activity; IEA:InterPro.
DR   GO; GO:0051701; P:biological process involved in interaction with host; IMP:MTBBASE.
DR   GO; GO:0006116; P:NADH oxidation; IEA:InterPro.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR023753; FAD/NAD-binding_dom.
DR   InterPro; IPR045024; NDH-2.
DR   PANTHER; PTHR43706; PTHR43706; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; FAD; Flavoprotein; Membrane; NAD;
KW   Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..470
FT                   /note="Type II NADH:quinone oxidoreductase NdhA"
FT                   /id="PRO_0000452728"
FT   TRANSMEM        389..409
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        184
FT                   /evidence="ECO:0000250|UniProtKB:Q2FZV7"
FT   BINDING         21..25
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:Q2FZV7"
FT   BINDING         89
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:Q2FZV7"
FT   BINDING         323
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:Q2FZV7"
FT   BINDING         334..335
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:Q2FZV7"
SQ   SEQUENCE   470 AA;  50385 MW;  584208FA80048828 CRC64;
     MTLSSGEPSA VGGRHRVVII GSGFGGLNAA KALKRADVDI TLISKTTTHL FQPLLYQVAT
     GILSEGDIAP TTRLILRRQK NVRVLLGEVN AIDLKAQTVT SKLMDMTTVT PYDSLIVAAG
     AQQSYFGNDE FATFAPGMKT IDDALELRGR ILGAFEAAEV STDHAERERR LTFVVVGAGP
     TGVEVAGQIV ELAERTLAGA FRTITPSECR VILLDAAPAV LPPMGPKLGL KAQRRLEKMD
     VEVQLNAMVT AVDYKGITIK EKDGGERRIE CACKVWAAGV AASPLGKMIA EGSDGTEIDR
     AGRVIVEPDL TVKGHPNVFV VGDLMFVPGV PGVAQGAIQG ARYATTVIKH MVKGNDDPAN
     RKPFHYFNKG SMATISRHSA VAQVGKLEFA GYFAWLAWLV LHLVYLVGYR NRIAALFAWG
     ISFMGRARGQ MAITSQMIYA RLVMTLMEQQ AQGALAAAEQ AEHAEQEAAG
 
 
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