NDHL_ARATH
ID NDHL_ARATH Reviewed; 191 AA.
AC Q9CAC5; Q0WMK1; Q9SSL2;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=NAD(P)H-quinone oxidoreductase subunit L, chloroplastic;
DE EC=7.1.1.- {ECO:0000305};
DE AltName: Full=NAD(P)H dehydrogenase subunit L {ECO:0000303|PubMed:21785130};
DE Short=NDH subunit L {ECO:0000305};
DE Short=NDH-L {ECO:0000305};
DE AltName: Full=NADH-plastoquinone oxidoreductase subunit L {ECO:0000305};
DE AltName: Full=Protein CHLORORESPIRATORY REDUCTION 23 {ECO:0000303|PubMed:18388109};
DE Flags: Precursor;
GN Name=ndhL {ECO:0000303|PubMed:21785130};
GN Synonyms=CRR23 {ECO:0000303|PubMed:18388109}, NDH-L {ECO:0000305};
GN OrderedLocusNames=At1g70760 {ECO:0000312|Araport:AT1G70760};
GN ORFNames=F15H11.1 {ECO:0000312|EMBL:AAD55491.1},
GN F5A18.6 {ECO:0000312|EMBL:AAG52336.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-191.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP COMPONENT OF THE NDH COMPLEX, DISRUPTION PHENOTYPE, AND SUBCELLULAR
RP LOCATION.
RX PubMed=18388109; DOI=10.1093/pcp/pcn058;
RA Shimizu H., Peng L., Myouga F., Motohashi R., Shinozaki K., Shikanai T.;
RT "CRR23/NdhL is a subunit of the chloroplast NAD(P)H dehydrogenase complex
RT in Arabidopsis.";
RL Plant Cell Physiol. 49:835-842(2008).
RN [6]
RP REVIEW.
RX PubMed=19995722; DOI=10.1093/mp/ssp052;
RA Suorsa M., Sirpioe S., Aro E.M.;
RT "Towards characterization of the chloroplast NAD(P)H dehydrogenase
RT complex.";
RL Mol. Plant 2:1127-1140(2009).
RN [7]
RP REVIEW.
RX PubMed=21029720; DOI=10.1016/j.bbabio.2010.10.015;
RA Peng L., Yamamoto H., Shikanai T.;
RT "Structure and biogenesis of the chloroplast NAD(P)H dehydrogenase
RT complex.";
RL Biochim. Biophys. Acta 1807:945-953(2011).
RN [8]
RP NOMENCLATURE, AND COMPONENT OF THE NDH COMPLEX.
RX PubMed=21785130; DOI=10.1093/pcp/pcr098;
RA Ifuku K., Endo T., Shikanai T., Aro E.M.;
RT "Structure of the chloroplast NADH dehydrogenase-like complex: nomenclature
RT for nuclear-encoded subunits.";
RL Plant Cell Physiol. 52:1560-1568(2011).
CC -!- FUNCTION: NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN
CC and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain
CC and possibly in a chloroplast respiratory chain. The immediate electron
CC acceptor for the enzyme in this species is believed to be
CC plastoquinone. Couples the redox reaction to proton translocation, and
CC thus conserves the redox energy in a proton gradient. {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADH = a plastoquinol + n
CC H(+)(out) + NAD(+); Xref=Rhea:RHEA:42608, Rhea:RHEA-COMP:9561,
CC Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62192;
CC Evidence={ECO:0000305};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADPH = a plastoquinol + n
CC H(+)(out) + NADP(+); Xref=Rhea:RHEA:42612, Rhea:RHEA-COMP:9561,
CC Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:62192;
CC Evidence={ECO:0000305};
CC -!- SUBUNIT: Part of the chloroplast NDH complex, composed of a mixture of
CC chloroplast and nucleus encoded subunits. Component of the NDH
CC subcomplex A, at least composed of ndhH, ndhI, ndhJ, ndhK, ndhL, ndhM,
CC ndhN and ndhO. {ECO:0000269|PubMed:18388109,
CC ECO:0000269|PubMed:21785130}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:18388109}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Malfunction of the NDH complex.
CC {ECO:0000269|PubMed:18388109}.
CC -!- SIMILARITY: Belongs to the NDH complex subunit L family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD55491.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC008148; AAD55491.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC011663; AAG52336.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35111.1; -; Genomic_DNA.
DR EMBL; BT010638; AAQ89660.1; -; mRNA.
DR EMBL; AK229819; BAF01650.1; -; mRNA.
DR PIR; B96732; B96732.
DR RefSeq; NP_177233.1; NM_105744.4.
DR PDB; 7WFG; EM; 4.33 A; L=1-191.
DR PDB; 7WG5; EM; 3.89 A; L=1-191.
DR PDBsum; 7WFG; -.
DR PDBsum; 7WG5; -.
DR AlphaFoldDB; Q9CAC5; -.
DR SMR; Q9CAC5; -.
DR IntAct; Q9CAC5; 43.
DR STRING; 3702.AT1G70760.1; -.
DR TCDB; 3.D.1.8.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR PaxDb; Q9CAC5; -.
DR PRIDE; Q9CAC5; -.
DR ProteomicsDB; 251126; -.
DR EnsemblPlants; AT1G70760.1; AT1G70760.1; AT1G70760.
DR GeneID; 843413; -.
DR Gramene; AT1G70760.1; AT1G70760.1; AT1G70760.
DR KEGG; ath:AT1G70760; -.
DR Araport; AT1G70760; -.
DR TAIR; locus:2013925; AT1G70760.
DR eggNOG; ENOG502S0BN; Eukaryota.
DR HOGENOM; CLU_103108_1_0_1; -.
DR InParanoid; Q9CAC5; -.
DR OMA; PWSTPEN; -.
DR OrthoDB; 1464671at2759; -.
DR PhylomeDB; Q9CAC5; -.
DR PRO; PR:Q9CAC5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CAC5; baseline and differential.
DR Genevisible; Q9CAC5; AT.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0010598; C:NAD(P)H dehydrogenase complex (plastoquinone); IDA:UniProtKB.
DR GO; GO:0042651; C:thylakoid membrane; IDA:TAIR.
DR GO; GO:0016655; F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor; IEA:InterPro.
DR GO; GO:0048038; F:quinone binding; IEA:UniProtKB-KW.
DR GO; GO:0010258; P:NADH dehydrogenase complex (plastoquinone) assembly; IMP:UniProtKB.
DR GO; GO:0009773; P:photosynthetic electron transport in photosystem I; TAS:TAIR.
DR InterPro; IPR019654; NADH-quinone_OxRdatse_su_L.
DR Pfam; PF10716; NdhL; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Membrane; NAD; NADP; Plastid; Plastoquinone;
KW Quinone; Reference proteome; Thylakoid; Transit peptide; Translocase;
KW Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..46
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 47..191
FT /note="NAD(P)H-quinone oxidoreductase subunit L,
FT chloroplastic"
FT /id="PRO_0000431813"
FT TRANSMEM 61..81
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..149
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
SQ SEQUENCE 191 AA; 21963 MW; E813C727C7580E94 CRC64;
MSRCGSLGLY APNALPSLSL KPRSVKSPFC ITSHTKPNDT LLHNVNKMRA KACDILGAKK
TILAAQLGAV LATIDHPALA ITGVNNQQEL SSVVLDIGII SVWYFLVMPP IIMNWLRVRW
YRRKFFEMYL QFMFVFMFFP GLLLWAPFLN FRKFPRDPNM KNPWDKPTDP DSIKNVYLKY
PYATPEDYDL D
