NDHN_ARATH
ID NDHN_ARATH Reviewed; 209 AA.
AC Q9LVM2; B3H5R4;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=NAD(P)H-quinone oxidoreductase subunit N, chloroplastic {ECO:0000305};
DE EC=7.1.1.- {ECO:0000305};
DE AltName: Full=NAD(P)H dehydrogenase subunit N {ECO:0000303|PubMed:21785130};
DE Short=NDH subunit N {ECO:0000305};
DE Short=NDH-N {ECO:0000303|PubMed:15608332};
DE AltName: Full=NADH-plastoquinone oxidoreductase subunit N {ECO:0000305};
DE Flags: Precursor;
GN Name=ndhN {ECO:0000303|PubMed:21785130};
GN Synonyms=NDH-N {ECO:0000303|PubMed:15608332};
GN OrderedLocusNames=At5g58260 {ECO:0000312|Araport:AT5G58260};
GN ORFNames=MCK7.13 {ECO:0000312|EMBL:BAA96917.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf {ECO:0000312|EMBL:BAH56820.1};
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP COMPONENT OF THE NDH COMPLEX, AND DISRUPTION PHENOTYPE.
RX PubMed=15608332; DOI=10.1105/tpc.104.028282;
RA Rumeau D., Becuwe-Linka N., Beyly A., Louwagie M., Garin J., Peltier G.;
RT "New subunits NDH-M, -N, and -O, encoded by nuclear genes, are essential
RT for plastid Ndh complex functioning in higher plants.";
RL Plant Cell 17:219-232(2005).
RN [6]
RP REVIEW.
RX PubMed=19995722; DOI=10.1093/mp/ssp052;
RA Suorsa M., Sirpioe S., Aro E.M.;
RT "Towards characterization of the chloroplast NAD(P)H dehydrogenase
RT complex.";
RL Mol. Plant 2:1127-1140(2009).
RN [7]
RP REVIEW.
RX PubMed=21029720; DOI=10.1016/j.bbabio.2010.10.015;
RA Peng L., Yamamoto H., Shikanai T.;
RT "Structure and biogenesis of the chloroplast NAD(P)H dehydrogenase
RT complex.";
RL Biochim. Biophys. Acta 1807:945-953(2011).
RN [8]
RP NOMENCLATURE, AND COMPONENT OF THE NDH COMPLEX.
RX PubMed=21785130; DOI=10.1093/pcp/pcr098;
RA Ifuku K., Endo T., Shikanai T., Aro E.M.;
RT "Structure of the chloroplast NADH dehydrogenase-like complex: nomenclature
RT for nuclear-encoded subunits.";
RL Plant Cell Physiol. 52:1560-1568(2011).
CC -!- FUNCTION: NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN
CC and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain
CC and possibly in a chloroplast respiratory chain. The immediate electron
CC acceptor for the enzyme in this species is believed to be
CC plastoquinone. Couples the redox reaction to proton translocation, and
CC thus conserves the redox energy in a proton gradient. {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADH = a plastoquinol + n
CC H(+)(out) + NAD(+); Xref=Rhea:RHEA:42608, Rhea:RHEA-COMP:9561,
CC Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62192;
CC Evidence={ECO:0000305};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADPH = a plastoquinol + n
CC H(+)(out) + NADP(+); Xref=Rhea:RHEA:42612, Rhea:RHEA-COMP:9561,
CC Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:62192;
CC Evidence={ECO:0000305};
CC -!- SUBUNIT: Part of the chloroplast NDH complex, composed of a mixture of
CC chloroplast and nucleus encoded subunits. Component of the NDH
CC subcomplex A, at least composed of ndhH, ndhI, ndhJ, ndhK, ndhL, ndhM,
CC ndhN and ndhO. {ECO:0000269|PubMed:15608332,
CC ECO:0000269|PubMed:21785130}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000250|UniProtKB:Q9CAC5}; Peripheral membrane protein
CC {ECO:0000305}; Stromal side {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9LVM2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9LVM2-2; Sequence=VSP_057441;
CC -!- DISRUPTION PHENOTYPE: Malfunction of the NDH complex.
CC {ECO:0000269|PubMed:15608332}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the NDH complex subunit N family. {ECO:0000305}.
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DR EMBL; AB019228; BAA96917.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97025.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97026.1; -; Genomic_DNA.
DR EMBL; AY080743; AAL85990.1; -; mRNA.
DR EMBL; AY113999; AAM45047.1; -; mRNA.
DR EMBL; AK318705; BAH56820.1; -; mRNA.
DR RefSeq; NP_001119457.1; NM_001125985.1. [Q9LVM2-2]
DR RefSeq; NP_200634.1; NM_125211.4. [Q9LVM2-1]
DR PDB; 7WFG; EM; 4.33 A; N=1-209.
DR PDB; 7WG5; EM; 3.89 A; N=1-209.
DR PDBsum; 7WFG; -.
DR PDBsum; 7WG5; -.
DR AlphaFoldDB; Q9LVM2; -.
DR SMR; Q9LVM2; -.
DR STRING; 3702.AT5G58260.1; -.
DR TCDB; 3.D.1.8.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR PaxDb; Q9LVM2; -.
DR PRIDE; Q9LVM2; -.
DR ProteomicsDB; 251319; -. [Q9LVM2-1]
DR EnsemblPlants; AT5G58260.1; AT5G58260.1; AT5G58260. [Q9LVM2-1]
DR EnsemblPlants; AT5G58260.2; AT5G58260.2; AT5G58260. [Q9LVM2-2]
DR GeneID; 835938; -.
DR Gramene; AT5G58260.1; AT5G58260.1; AT5G58260. [Q9LVM2-1]
DR Gramene; AT5G58260.2; AT5G58260.2; AT5G58260. [Q9LVM2-2]
DR KEGG; ath:AT5G58260; -.
DR Araport; AT5G58260; -.
DR TAIR; locus:2161263; AT5G58260.
DR eggNOG; ENOG502QUZI; Eukaryota.
DR HOGENOM; CLU_087432_0_0_1; -.
DR InParanoid; Q9LVM2; -.
DR OMA; HGIRPPH; -.
DR PhylomeDB; Q9LVM2; -.
DR PRO; PR:Q9LVM2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LVM2; baseline and differential.
DR Genevisible; Q9LVM2; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0010598; C:NAD(P)H dehydrogenase complex (plastoquinone); TAS:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0016655; F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor; IEA:InterPro.
DR GO; GO:0048038; F:quinone binding; IEA:UniProtKB-KW.
DR GO; GO:0050832; P:defense response to fungus; IDA:TAIR.
DR GO; GO:0010258; P:NADH dehydrogenase complex (plastoquinone) assembly; IMP:TAIR.
DR InterPro; IPR020874; NAD(P)H-quinone_OxRdtase_su_N.
DR PANTHER; PTHR35515; PTHR35515; 1.
DR Pfam; PF11909; NdhN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Chloroplast; Membrane; NAD; NADP;
KW Plastid; Plastoquinone; Quinone; Reference proteome; Thylakoid;
KW Transit peptide; Translocase; Transport.
FT TRANSIT 1..45
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 46..209
FT /note="NAD(P)H-quinone oxidoreductase subunit N,
FT chloroplastic"
FT /id="PRO_0000431814"
FT VAR_SEQ 164..209
FT /note="Missing (in isoform 2)"
FT /id="VSP_057441"
SQ SEQUENCE 209 AA; 23398 MW; A8A621C6CA7B97D7 CRC64;
MGSRAICIQR VAPPCFEASQ VKKIKTVGSF LVNTRSKRRR STGVKCSSIA DYIGGDLVKP
DIGQWLQDVE EHKAIAIYAP HEGGYEGRYL NRLKMQGYYF LDISARGLGD PETTLLKNYP
VCPAHLGKQP IARWYYPPEV DYRLAALPPS AKGLVVWVLE AKVLSKSELQ FLALLPSLRP
NVRVIAECGN WRKFVWKPLA EIANLAAQE
