ID   NDH_DICDI               Reviewed;         451 AA.
AC   Q55CD9;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 2.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Probable NADH dehydrogenase;
DE            EC=7.1.1.2;
GN   ORFNames=DDB_G0270104;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the NADH dehydrogenase family. {ECO:0000305}.
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DR   EMBL; AAFI02000005; EAL72402.2; -; Genomic_DNA.
DR   RefSeq; XP_646542.2; XM_641450.2.
DR   AlphaFoldDB; Q55CD9; -.
DR   SMR; Q55CD9; -.
DR   STRING; 44689.DDB0238855; -.
DR   PaxDb; Q55CD9; -.
DR   EnsemblProtists; EAL72402; EAL72402; DDB_G0270104.
DR   GeneID; 8617508; -.
DR   KEGG; ddi:DDB_G0270104; -.
DR   dictyBase; DDB_G0270104; -.
DR   eggNOG; KOG2495; Eukaryota.
DR   HOGENOM; CLU_021377_1_3_1; -.
DR   InParanoid; Q55CD9; -.
DR   OMA; DHCIFLD; -.
DR   PhylomeDB; Q55CD9; -.
DR   PRO; PR:Q55CD9; -.
DR   Proteomes; UP000002195; Chromosome 1.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006116; P:NADH oxidation; IEA:InterPro.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR023753; FAD/NAD-binding_dom.
DR   InterPro; IPR045024; NDH-2.
DR   PANTHER; PTHR43706; PTHR43706; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   SUPFAM; SSF51905; SSF51905; 2.
PE   3: Inferred from homology;
KW   FAD; Flavoprotein; NAD; Oxidoreductase; Reference proteome; Translocase.
FT   CHAIN           1..451
FT                   /note="Probable NADH dehydrogenase"
FT                   /id="PRO_0000327518"
FT   BINDING         41..71
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   BINDING         199..236
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   451 AA;  51141 MW;  E13D27DFA4848BFF CRC64;
     MGLKNLIIRG RNYISDHLTK SSLEKAIIRR QKRGKVIENE KLIILGCGWG SYSFLKNLNS
     IKYDITVISP RNHFLFTPLL TSSAVGTLEF RSIAEPVRTT RDINEFKYIQ ASVTSINPEN
     NSVLVKSTFH NEKPFEMKYD KLVIGVGSRN NTFGIKGVEE NANFLKELHH AREIRQKIIE
     CFERASLPDV STEERERLLS FVIVGGGATG IEFTSELNDF FSEDLSRLFP FVPVNEVKII
     LLEASGKILS TFDQKLVKKA LINFRNSGID VRTHSSVKEV LKDYVILDNG DRIPYGLLVW
     STGIGQHPLV KNSSFEKDSH DRIIVDDHLR VKNYSNVFSF GDCANVENKN YPPTAQVASQ
     SAVYLAKEFN NLEKLNPNPP KPFAFKFLGL LAYTGKKSGI LQTDFFDLSG FIGFITWRSA
     YLTRLGSLRS KIQVPFDWMR TLIFGRDISS F