ID   NDH_STAES               Reviewed;         402 AA.
AC   Q8CPV5;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Type II NADH:quinone oxidoreductase {ECO:0000250|UniProtKB:Q2FZV7};
DE            EC=1.6.5.9 {ECO:0000250|UniProtKB:Q2FZV7};
DE   AltName: Full=NDH-2 {ECO:0000250|UniProtKB:Q2FZV7};
GN   OrderedLocusNames=SE_0635;
OS   Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=176280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 12228 / FDA PCI 1200;
RX   PubMed=12950922; DOI=10.1046/j.1365-2958.2003.03671.x;
RA   Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q.,
RA   Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H.,
RA   Zhao G.-P., Qu D., Danchin A., Wen Y.-M.;
RT   "Genome-based analysis of virulence genes in a non-biofilm-forming
RT   Staphylococcus epidermidis strain (ATCC 12228).";
RL   Mol. Microbiol. 49:1577-1593(2003).
CC   -!- FUNCTION: Alternative, nonproton pumping NADH:quinone oxidoreductase
CC       that delivers electrons to the respiratory chain by oxidation of NADH
CC       and reduction of quinones, and contributes to the regeneration of
CC       NAD(+). {ECO:0000250|UniProtKB:Q2FZV7}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a quinone + H(+) + NADH = a quinol + NAD(+);
CC         Xref=Rhea:RHEA:46160, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124; EC=1.6.5.9;
CC         Evidence={ECO:0000250|UniProtKB:Q2FZV7};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000250|UniProtKB:Q2FZV7};
CC       Note=Binds 1 FAD per subunit. {ECO:0000250|UniProtKB:Q2FZV7};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q2FZV7}.
CC   -!- SIMILARITY: Belongs to the NADH dehydrogenase family. {ECO:0000305}.
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DR   EMBL; AE015929; AAO04232.1; -; Genomic_DNA.
DR   RefSeq; NP_764190.1; NC_004461.1.
DR   RefSeq; WP_011082633.1; NZ_WBME01000044.1.
DR   AlphaFoldDB; Q8CPV5; -.
DR   SMR; Q8CPV5; -.
DR   STRING; 176280.SE_0635; -.
DR   EnsemblBacteria; AAO04232; AAO04232; SE_0635.
DR   KEGG; sep:SE_0635; -.
DR   PATRIC; fig|176280.10.peg.608; -.
DR   eggNOG; COG1252; Bacteria.
DR   HOGENOM; CLU_021377_7_2_9; -.
DR   OMA; DHCIFLD; -.
DR   Proteomes; UP000001411; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0050136; F:NADH dehydrogenase (quinone) activity; IEA:RHEA.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR023753; FAD/NAD-binding_dom.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   SUPFAM; SSF51905; SSF51905; 2.
PE   3: Inferred from homology;
KW   Cell membrane; FAD; Flavoprotein; Membrane; NAD; Oxidoreductase.
FT   CHAIN           1..402
FT                   /note="Type II NADH:quinone oxidoreductase"
FT                   /id="PRO_0000287373"
FT   ACT_SITE        172
FT                   /evidence="ECO:0000250|UniProtKB:Q2FZV7"
FT   BINDING         12..16
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:Q2FZV7"
FT   BINDING         39..40
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:Q2FZV7"
FT   BINDING         83
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:Q2FZV7"
FT   BINDING         302
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:Q2FZV7"
FT   BINDING         319..320
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:Q2FZV7"
FT   BINDING         379
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:Q2FZV7"
SQ   SEQUENCE   402 AA;  44255 MW;  662B59D0D7AD1281 CRC64;
     MAQDRKKVLV LGAGYAGLQT VTKLQKELSA DAAEITLINK NEYHYESTWL HEASAGTINY
     EDLLYPVEKT VNKNKVNFVV AEVTKIDRNA KRVETDKGVY DFDILVVALG FVSETFGIDG
     MKEHAFQIEN VLTSRKLSRH IEDKFANYAA SKEKDDKDLS ILVGGAGFTG IEFLGELTDR
     IPELCSKYGV DQSKVKLTCV EAAPKMLPMF SDDLVSYAVK YLEDRGVEFK IATPIVACNE
     KGFVVEVNGE KQQLEAGTSV WTAGVRGSHL MEESFEGVKR GRIINKQDLT IEGHNDIFVI
     GDCSAFIPAD EERPLPTTAQ IAMQQGEHTA SNIKRLLNGE STQDFQYVNR GTVCSLGAND
     GVGIVYGRDI AGKKAAFLKK VIDTRAIYKL GGIGLAFKKG KF