NDK5_MOUSE
ID NDK5_MOUSE Reviewed; 211 AA.
AC Q99MH5; Q810R1;
DT 16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2003, sequence version 2.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Nucleoside diphosphate kinase homolog 5;
DE Short=NDK-H 5;
DE Short=NDP kinase homolog 5;
DE AltName: Full=Rsph23 {ECO:0000303|PubMed:32203505};
DE AltName: Full=nm23-M5;
GN Name=Nme5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=ICR; TISSUE=Testis;
RX PubMed=12788088; DOI=10.1016/s0006-291x(03)00916-1;
RA Hwang K.-C., Ok D.-W., Hong J.-C., Kim M.-O., Kim J.-H.;
RT "Cloning, sequencing, and characterization of the murine nm23-M5 gene
RT during mouse spermatogenesis and spermiogenesis.";
RL Biochem. Biophys. Res. Commun. 306:198-207(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=32203505; DOI=10.1371/journal.pgen.1008664;
RA Yoke H., Ueno H., Narita A., Sakai T., Horiuchi K., Shingyoji C.,
RA Hamada H., Shinohara K.;
RT "Rsph4a is essential for the triplet radial spoke head assembly of the
RT mouse motile cilia.";
RL PLoS Genet. 16:e1008664-e1008664(2020).
CC -!- FUNCTION: Does not seem to have NDK kinase activity. Confers protection
CC from cell death by Bax and alters the cellular levels of several
CC antioxidant enzymes including Gpx5. May play a role in spermiogenesis
CC by increasing the ability of late-stage spermatids to eliminate
CC reactive oxygen species. {ECO:0000269|PubMed:12788088}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC {ECO:0000269|PubMed:32203505}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=Additional isoforms seem to exist.;
CC Name=1;
CC IsoId=Q99MH5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q99MH5-2; Sequence=VSP_007773, VSP_007774;
CC -!- TISSUE SPECIFICITY: Expressed in the trachea, ependymal cells and
CC oviduct (at protein level) (PubMed:32203505). Expressed predominantly
CC in germ cells of the testis. Not expressed in testicular somatic cells.
CC {ECO:0000269|PubMed:12788088, ECO:0000269|PubMed:32203505}.
CC -!- DEVELOPMENTAL STAGE: First appears in pachytene spermatocytes and
CC increases in abundance in subsequent stages.
CC {ECO:0000269|PubMed:12788088}.
CC -!- SIMILARITY: Belongs to the NDK family. {ECO:0000305}.
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DR EMBL; AF343565; AAK20866.1; -; mRNA.
DR EMBL; BC049625; AAH49625.1; -; mRNA.
DR CCDS; CCDS29130.1; -. [Q99MH5-1]
DR RefSeq; NP_542368.2; NM_080637.3. [Q99MH5-1]
DR RefSeq; XP_017173493.1; XM_017318004.1.
DR AlphaFoldDB; Q99MH5; -.
DR SMR; Q99MH5; -.
DR STRING; 10090.ENSMUSP00000078269; -.
DR PhosphoSitePlus; Q99MH5; -.
DR MaxQB; Q99MH5; -.
DR PaxDb; Q99MH5; -.
DR PRIDE; Q99MH5; -.
DR ProteomicsDB; 252931; -. [Q99MH5-1]
DR ProteomicsDB; 252932; -. [Q99MH5-2]
DR Antibodypedia; 26568; 184 antibodies from 25 providers.
DR DNASU; 75533; -.
DR Ensembl; ENSMUST00000079287; ENSMUSP00000078269; ENSMUSG00000035984. [Q99MH5-1]
DR Ensembl; ENSMUST00000134875; ENSMUSP00000118213; ENSMUSG00000035984. [Q99MH5-2]
DR GeneID; 75533; -.
DR KEGG; mmu:75533; -.
DR UCSC; uc008eks.1; mouse. [Q99MH5-1]
DR CTD; 8382; -.
DR MGI; MGI:1922783; Nme5.
DR VEuPathDB; HostDB:ENSMUSG00000035984; -.
DR eggNOG; KOG0888; Eukaryota.
DR GeneTree; ENSGT00940000159595; -.
DR HOGENOM; CLU_060216_8_3_1; -.
DR InParanoid; Q99MH5; -.
DR OMA; HNAISYW; -.
DR PhylomeDB; Q99MH5; -.
DR TreeFam; TF354225; -.
DR BioGRID-ORCS; 75533; 0 hits in 74 CRISPR screens.
DR ChiTaRS; Nme5; mouse.
DR PRO; PR:Q99MH5; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q99MH5; protein.
DR Bgee; ENSMUSG00000035984; Expressed in spermatid and 192 other tissues.
DR ExpressionAtlas; Q99MH5; baseline and differential.
DR Genevisible; Q99MH5; MM.
DR GO; GO:0005929; C:cilium; IDA:UniProtKB.
DR GO; GO:0005576; C:extracellular region; IEA:GOC.
DR GO; GO:0036126; C:sperm flagellum; IDA:MGI.
DR GO; GO:0004550; F:nucleoside diphosphate kinase activity; IEA:InterPro.
DR GO; GO:0060271; P:cilium assembly; IMP:MGI.
DR GO; GO:0003341; P:cilium movement; IBA:GO_Central.
DR GO; GO:0006241; P:CTP biosynthetic process; IEA:InterPro.
DR GO; GO:0003351; P:epithelial cilium movement involved in extracellular fluid movement; IMP:MGI.
DR GO; GO:0051649; P:establishment of localization in cell; IMP:MGI.
DR GO; GO:0006183; P:GTP biosynthetic process; IEA:InterPro.
DR GO; GO:1902176; P:negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway; IDA:MGI.
DR GO; GO:0006165; P:nucleoside diphosphate phosphorylation; IEA:InterPro.
DR GO; GO:0000302; P:response to reactive oxygen species; NAS:UniProtKB.
DR GO; GO:0007286; P:spermatid development; IDA:UniProtKB.
DR GO; GO:0006228; P:UTP biosynthetic process; IEA:InterPro.
DR GO; GO:0021591; P:ventricular system development; IMP:MGI.
DR Gene3D; 3.30.70.141; -; 1.
DR InterPro; IPR007858; Dpy-30_motif.
DR InterPro; IPR034907; NDK-like_dom.
DR InterPro; IPR036850; NDK-like_dom_sf.
DR InterPro; IPR012410; NDK_H5.
DR InterPro; IPR001564; Nucleoside_diP_kinase.
DR Pfam; PF05186; Dpy-30; 1.
DR Pfam; PF00334; NDK; 1.
DR PIRSF; PIRSF036504; NDK_H5; 1.
DR PRINTS; PR01243; NUCDPKINASE.
DR SMART; SM00562; NDK; 1.
DR SUPFAM; SSF54919; SSF54919; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Developmental protein;
KW Differentiation; Reference proteome; Spermatogenesis.
FT CHAIN 1..211
FT /note="Nucleoside diphosphate kinase homolog 5"
FT /id="PRO_0000137126"
FT ACT_SITE 127
FT /note="Pros-phosphohistidine intermediate"
FT /evidence="ECO:0000255"
FT VAR_SEQ 113..114
FT /note="LR -> DY (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12788088,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_007773"
FT VAR_SEQ 115..211
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12788088,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_007774"
FT CONFLICT 2
FT /note="E -> V (in Ref. 1; AAK20866)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 211 AA; 24002 MW; 3624D0C76BDF3ADD CRC64;
MEVSMPLPQI YVEKTLALIK PDVVDKEEEI QDIILGSGFT IIQRRKLHLS PEHCSNFYVE
QYGKMFFPNL TAYMSSGPLV AMILARHKAI SYWKELMGPS NSLVAKETHP DSLRAIYGTD
ELRNALHGSN DFAASEREIR FMFPAVIIEP IPIGQAAKDY INLYVAPTLL QGLTELCKEK
PPDPYLWLAD WLMKNNPNKP KLCHFPVTEE P
