ID   13S1_FAGES              Reviewed;         565 AA.
AC   O23878;
DT   11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=13S globulin seed storage protein 1;
DE   AltName: Full=Legumin-like protein 1;
DE   Contains:
DE     RecName: Full=13S globulin seed storage protein 1 acidic chain;
DE   Contains:
DE     RecName: Full=13S globulin seed storage protein 1 basic chain;
DE   Flags: Precursor;
GN   Name=FA02;
OS   Fagopyrum esculentum (Common buckwheat) (Polygonum fagopyrum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Polygonaceae; Polygonoideae; Fagopyreae; Fagopyrum.
OX   NCBI_TaxID=3617;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC   STRAIN=cv. Kitayuki; TISSUE=Immature seed;
RX   PubMed=11308332; DOI=10.1021/jf0011485;
RA   Fujino K., Funatsuki H., Inada M., Shimono Y., Kikuta Y.;
RT   "Expression, cloning, and immunological analysis of buckwheat (Fagopyrum
RT   esculentum Moench) seed storage proteins.";
RL   J. Agric. Food Chem. 49:1825-1829(2001).
CC   -!- FUNCTION: Seed storage protein.
CC   -!- SUBUNIT: Hexamer; each subunit is composed of an acidic and a basic
CC       chain derived from a single precursor and linked by a disulfide bond.
CC       {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed only in immature seeds.
CC       {ECO:0000269|PubMed:11308332}.
CC   -!- DEVELOPMENTAL STAGE: Expressed between 7 and 28 days after pollination.
CC       {ECO:0000269|PubMed:11308332}.
CC   -!- MISCELLANEOUS: The sequence of the probable beta chain is highly
CC       homologous to the N-terminal sequence of BW24KD, a major buckwheat
CC       allergen.
CC   -!- SIMILARITY: Belongs to the 11S seed storage protein (globulins) family.
CC       {ECO:0000305}.
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DR   EMBL; D87980; BAA21758.1; -; mRNA.
DR   PIR; T10696; T10696.
DR   AlphaFoldDB; O23878; -.
DR   SMR; O23878; -.
DR   Allergome; 698; Fag e 1.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.10; -; 3.
DR   InterPro; IPR006044; 11S_seedstore_pln.
DR   InterPro; IPR006045; Cupin_1.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF00190; Cupin_1; 2.
DR   PRINTS; PR00439; 11SGLOBULIN.
DR   SMART; SM00835; Cupin_1; 2.
DR   SUPFAM; SSF51182; SSF51182; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Seed storage protein; Signal; Storage protein.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..377
FT                   /note="13S globulin seed storage protein 1 acidic chain"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000032003"
FT   CHAIN           378..565
FT                   /note="13S globulin seed storage protein 1 basic chain"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000032004"
FT   DOMAIN          49..331
FT                   /note="Cupin type-1 1"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          390..539
FT                   /note="Cupin type-1 2"
FT                   /evidence="ECO:0000255"
FT   REGION          126..224
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          271..301
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          356..376
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        133..222
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        274..301
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        356..374
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        44..77
FT                   /evidence="ECO:0000250"
FT   DISULFID        120..384
FT                   /note="Interchain (between acidic and basic chains)"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   565 AA;  64518 MW;  2DD7FCC64E3CD4F0 CRC64;
     MSTKLILSFS LCLMVLSCSA QLLPWRKGQR SRPHRGHQQF HHQCDVQRLT ASEPSRRVRS
     EAGVTEIWDN DTPEFRCAGF VAVRVVIQPG GLLLPSYSNA PYITFVEQGR GVQGVVVPGC
     PETFQSESEF EYPQSQRDQR SRQSESEESS RGDQRTRQSE SEEFSRGDQR TRQSESEEFS
     RGDQRTRQSE SEEFSRGDQR TRQSESEEFS RGDQHQKIFR IRDGDVIPSP AGVVQWTHND
     GDNDLISITL YDANSFQNQL DGNVRNFFLA GQSKQSREDR RSQRQTREEG SDRQSRESDD
     DEALLEANIL TGFQDEILQE IFRNVDQETI SKLRGDNDQR GFIVQARDLK LRVPEEYEEE
     LQRERGDRKR GGSGRSNGLE QAFCNLKFKQ NVNRPSRADV FNPRAGRINT VNSNNLPILE
     FIQLSAQHVV LYKNAILGPR WNLNAHSALY VTRGEGRVQV VGDEGRSVFD DNVQRGQILV
     VPQGFAVVLK AGREGLEWVE LKNDDNAITS PIAGKTSVLR AIPVEVLANS YDISTKEAFR
     LKNGRQEVEV FLPFQSRDEK ERERF