ID   NDMC_PSEDT              Reviewed;         282 AA.
AC   F0E1K6;
DT   29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   03-MAY-2011, sequence version 1.
DT   25-MAY-2022, entry version 27.
DE   RecName: Full=Probable methylxanthine N7-demethylase NdmC;
DE            EC=1.14.13.128;
DE   AltName: Full=7-methylxanthine demethylase;
GN   ORFNames=G1E_06898;
OS   Pseudomonas sp. (strain TJI-51).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=985010;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=TJI-51;
RA   Asif H., Azim M.K., Khan A.;
RT   "A draft genome sequence and comparative analysis of Pseudomonas putida
RT   TJI-51.";
RL   Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=TJI-51;
RX   PubMed=22328667; DOI=10.1128/jb.06637-11;
RA   Summers R.M., Louie T.M., Yu C.L., Gakhar L., Louie K.C., Subramanian M.;
RT   "Novel, highly specific N-demethylases enable bacteria to live on caffeine
RT   and related purine alkaloids.";
RL   J. Bacteriol. 194:2041-2049(2012).
CC   -!- FUNCTION: Involved in the caffeine degradation, which is the essential
CC       first step for assimilating the carbon and nitrogen in caffeine.
CC       Probably catalyzes the N7-demethylation of 7-methylxanthine to produce
CC       xanthine and formaldehyde. {ECO:0000269|PubMed:22328667}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=7-methylxanthine + H(+) + NADPH + O2 = formaldehyde + H2O +
CC         NADP(+) + xanthine; Xref=Rhea:RHEA:30307, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16842,
CC         ChEBI:CHEBI:17712, ChEBI:CHEBI:48991, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349; EC=1.14.13.128;
CC         Evidence={ECO:0000269|PubMed:22328667};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=7-methylxanthine + H(+) + NADH + O2 = formaldehyde + H2O +
CC         NAD(+) + xanthine; Xref=Rhea:RHEA:30311, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16842,
CC         ChEBI:CHEBI:17712, ChEBI:CHEBI:48991, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.14.13.128;
CC         Evidence={ECO:0000269|PubMed:22328667};
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DR   EMBL; AEWE02000020; EGB99694.1; -; Genomic_DNA.
DR   RefSeq; WP_009682459.1; NZ_AEWE02000020.1.
DR   AlphaFoldDB; F0E1K6; -.
DR   SMR; F0E1K6; -.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009822; P:alkaloid catabolic process; IDA:UniProtKB.
DR   InterPro; IPR044043; VanA_C_cat.
DR   Pfam; PF19112; VanA_C; 1.
PE   1: Evidence at protein level;
KW   Alkaloid metabolism; NAD; Oxidoreductase.
FT   CHAIN           1..282
FT                   /note="Probable methylxanthine N7-demethylase NdmC"
FT                   /id="PRO_0000422369"
SQ   SEQUENCE   282 AA;  31473 MW;  18040EB9F9D50516 CRC64;
     MHAENSFVID DWYPVGALAE TVSGRKYHTR ILGTEIWYQL ADGTVSAGLA DNTAELASKS
     IYGLLWVSLS DNPRDVIAIP EFAEADRRVV SAGSIRVATS GLRVIENFLD MAHFPFVHTD
     ILGAEPLTEV AAYDVEIDEA ADEIRAVNCR FPQPKGSAAA SEPVEMQYVY RIARPFIAIL
     YKTCVIDANR LDVLGLFVQP VDQESSIAHT IMCYLDDINT DKQLRDFQQR IFGQDIMILI
     NQVPKALPLN PRHETPVRAD ALSSAYRRWL NDRNVTFGTT RG