ID   NDNF_XENTR              Reviewed;         570 AA.
AC   A4IIK7; B1H2I6;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 2.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Protein NDNF;
DE   Flags: Precursor;
GN   Name=ndnf; ORFNames=TNeu083d09;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Neurula;
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Secretory protein that plays a role in various cellular
CC       processes. Acts as a chemorepellent acting on gonadotropin-releasing
CC       hormone (GnRH) expressing neurons regulating their migration to the
CC       hypothalamus. Also promotes neuron migration, growth and survival as
CC       well as neurite outgrowth and is involved in the development of the
CC       olfactory system. May also act through the regulation of growth factors
CC       activity and downstream signaling (By similarity). Also regulates
CC       extracellular matrix assembly and cell adhesiveness (By similarity).
CC       Promotes endothelial cell survival, vessel formation and plays an
CC       important role in the process of revascularization through NOS3-
CC       dependent mechanisms (By similarity). {ECO:0000250|UniProtKB:Q8C119,
CC       ECO:0000250|UniProtKB:Q8TB73}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q8C119}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAI36061.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};
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DR   EMBL; CR942677; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; BC136060; AAI36061.1; ALT_SEQ; mRNA.
DR   EMBL; BC161015; AAI61015.1; -; mRNA.
DR   RefSeq; NP_001116272.1; NM_001122800.1.
DR   RefSeq; XP_012821337.1; XM_012965883.2.
DR   AlphaFoldDB; A4IIK7; -.
DR   STRING; 8364.ENSXETP00000004498; -.
DR   PaxDb; A4IIK7; -.
DR   GeneID; 100125014; -.
DR   KEGG; xtr:100125014; -.
DR   CTD; 79625; -.
DR   Xenbase; XB-GENE-1016849; ndnf.
DR   eggNOG; KOG4806; Eukaryota.
DR   InParanoid; A4IIK7; -.
DR   OrthoDB; 294977at2759; -.
DR   Proteomes; UP000008143; Chromosome 1.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000002103; Expressed in brain and 10 other tissues.
DR   GO; GO:0031012; C:extracellular matrix; ISS:UniProtKB.
DR   GO; GO:0005576; C:extracellular region; ISS:UniProtKB.
DR   GO; GO:0005539; F:glycosaminoglycan binding; ISS:UniProtKB.
DR   GO; GO:0008201; F:heparin binding; ISS:UniProtKB.
DR   GO; GO:0044344; P:cellular response to fibroblast growth factor stimulus; ISS:UniProtKB.
DR   GO; GO:0030198; P:extracellular matrix organization; ISS:UniProtKB.
DR   GO; GO:0021828; P:gonadotrophin-releasing hormone neuronal migration to the hypothalamus; ISS:UniProtKB.
DR   GO; GO:0043524; P:negative regulation of neuron apoptotic process; ISS:UniProtKB.
DR   GO; GO:0001764; P:neuron migration; ISS:UniProtKB.
DR   GO; GO:0019800; P:peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; ISS:UniProtKB.
DR   GO; GO:0010811; P:positive regulation of cell-substrate adhesion; ISS:UniProtKB.
DR   GO; GO:0010976; P:positive regulation of neuron projection development; ISS:UniProtKB.
DR   CDD; cd00063; FN3; 1.
DR   Gene3D; 2.60.40.10; -; 1.
DR   InterPro; IPR003961; FN3_dom.
DR   InterPro; IPR036116; FN3_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR019326; NDNF.
DR   InterPro; IPR045805; NDNF_C.
DR   PANTHER; PTHR14619; PTHR14619; 1.
DR   Pfam; PF10179; NDNF; 1.
DR   Pfam; PF19433; NDNF_C; 1.
DR   SMART; SM00060; FN3; 2.
DR   SUPFAM; SSF49265; SSF49265; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Neurogenesis; Reference proteome; Repeat; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..570
FT                   /note="Protein NDNF"
FT                   /id="PRO_0000301968"
FT   DOMAIN          261..333
FT                   /note="Fibronectin type-III 1"
FT   DOMAIN          447..566
FT                   /note="Fibronectin type-III 2"
FT   CARBOHYD        324
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   570 AA;  64752 MW;  CBC0A31823EBCDAA CRC64;
     MKLCPWYIAL ILLPVCLQSQ KLPTRDEELF QMQIRDKALF HDSSVIPDGA EISGYLFRDN
     PKRYFFVVEE DNTPLSVIVT PCDAPLEWKL TLQELPEEAS GEGSGEPEPL EEQKQQIVNE
     EGTELFSYKG NDVEYFVSSS SPSGLYQLEL ISTEKDTHFK VYATTTPESD QPYPELPYDP
     RVDVTSLGRT TVTLAWKPTP TSSVMKQPIQ YCVVINKEHN FKSICAVEAK MNADDAFMMV
     PKPGIDFNPF DFAHFGFQSD NNAGKDRNFM PKASSSKLLR QITTKPKVDI QKVCIGNKNI
     FTVSDLKPDT QYYFDVFAVN AATNMSTAYV GTFARTKEEA KQKTVELKDG KVTDVFIKRK
     GTKFLRFSPV SSHQKVTFSV HSCLDAIQIQ VRRDGKLLLS HSVEGVRQFQ LRGKPKAKYL
     IRLKGSKKGA SMLKILATSK FNKQPFPSLP EDTRIKAFDK LRTCSSVTVA WLGTQERNKF
     CVYKKEVDDD YTEEHKKREQ NQCLGPDTRK KTEKVLCKYF HSQNIHKAVT TETIKGLQPG
     KSYMLDVYVM GHGGHSVKYQ SKIVKTRKFC