NDR1_ARATH
ID NDR1_ARATH Reviewed; 219 AA.
AC O48915;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Protein NDR1;
DE AltName: Full=Non-race specific disease resistance protein 1;
DE Short=AtNDR1;
DE Flags: Precursor;
GN Name=NDR1; OrderedLocusNames=At3g20600; ORFNames=K10D20.24;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RX PubMed=9395402; DOI=10.1126/science.278.5345.1963;
RA Century K.S., Shapiro A.D., Repetti P.P., Dahlbeck D., Holub E.,
RA Staskawicz B.J.;
RT "NDR1, a pathogen-induced component required for Arabidopsis disease
RT resistance.";
RL Science 278:1963-1965(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Aa-0, cv. Columbia, cv. Ei-2, and cv. Nd-1;
RX PubMed=19064707; DOI=10.1534/genetics.108.097279;
RA Caldwell K.S., Michelmore R.W.;
RT "Arabidopsis thaliana genes encoding defense signaling and recognition
RT proteins exhibit contrasting evolutionary dynamics.";
RL Genetics 181:671-684(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=11607554; DOI=10.1073/pnas.92.14.6597;
RA Century K.S., Holub E.B., Staskawicz B.J.;
RT "NDR1, a locus of Arabidopsis thaliana that is required for disease
RT resistance to both a bacterial and a fungal pathogen.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:6597-6601(1995).
RN [7]
RP FUNCTION.
RX PubMed=11706189; DOI=10.1104/pp.010096;
RA Shapiro A.D., Zhang C.;
RT "The role of NDR1 in avirulence gene-directed signaling and control of
RT programmed cell death in Arabidopsis.";
RL Plant Physiol. 127:1089-1101(2001).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, GPI-ANCHOR, AND GLYCOSYLATION.
RX PubMed=15447649; DOI=10.1111/j.1365-313x.2004.02203.x;
RA Coppinger P., Repetti P.P., Day B., Dahlbeck D., Mehlert A.,
RA Staskawicz B.J.;
RT "Overexpression of the plasma membrane-localized NDR1 protein results in
RT enhanced bacterial disease resistance in Arabidopsis thaliana.";
RL Plant J. 40:225-237(2004).
RN [9]
RP FUNCTION.
RX PubMed=16941900; DOI=10.1094/mpmi-19-0958;
RA Johansson A., Staal J., Dixelius C.;
RT "Early responses in the Arabidopsis-Verticillium longisporum pathosystem
RT are dependent on NDR1, JA- and ET-associated signals via cytosolic NPR1 and
RT RFO1.";
RL Mol. Plant Microbe Interact. 19:958-969(2006).
RN [10]
RP FUNCTION, INTERACTION WITH RIN4, AND MUTAGENESIS OF MET-1; ASN-2; ASN-3;
RP GLN-4; ASN-5; GLU-6; ASP-7 AND THR-8.
RX PubMed=17012600; DOI=10.1105/tpc.106.044693;
RA Day B., Dahlbeck D., Staskawicz B.J.;
RT "NDR1 interaction with RIN4 mediates the differential activation of
RT multiple disease resistance pathways in Arabidopsis.";
RL Plant Cell 18:2782-2791(2006).
CC -!- FUNCTION: Involved in disease resistance. Required for resistance
CC conferred by multiple R genes recognizing different bacterial and
CC oomycete pathogen isolates like avirulent P.syringae or H.parasitica
CC (downy mildew). Required for the establishment of hypersensitive
CC response (HR) and systemic acquired resistance (SAR) after infection
CC with the bacterial pathogen P.syringae DC3000 carrying avrRpt2.
CC Required for resistance to the soilborne fungus V.longisporum.
CC Interaction with RIN4 is required for the activation of the R gene RPS2
CC and RPS2-mediated resistance. {ECO:0000269|PubMed:11607554,
CC ECO:0000269|PubMed:11706189, ECO:0000269|PubMed:15447649,
CC ECO:0000269|PubMed:16941900, ECO:0000269|PubMed:17012600}.
CC -!- SUBUNIT: Interacts with RIN4 (via C-terminus).
CC {ECO:0000269|PubMed:17012600}.
CC -!- INTERACTION:
CC O48915; Q8GYN5: RIN4; NbExp=3; IntAct=EBI-2314492, EBI-2270391;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:15447649};
CC Lipid-anchor, GPI-anchor {ECO:0000305|PubMed:15447649}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:15447649}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC condition. In case of infection, plants loose R gene resistance
CC mediated by RPM1, RPS2, RPS5. Plants overexpressing NDR1 show enhanced
CC resistance against the virulent strain of the bacterial pathogen Pst
CC DC3000. {ECO:0000269|PubMed:11607554}.
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DR EMBL; AF021346; AAB95208.1; -; Genomic_DNA.
DR EMBL; EF470696; ABR45925.1; -; Genomic_DNA.
DR EMBL; EF470697; ABR45926.1; -; Genomic_DNA.
DR EMBL; EF470704; ABR45933.1; -; Genomic_DNA.
DR EMBL; EF470706; ABR45935.1; -; Genomic_DNA.
DR EMBL; AP000410; BAB01168.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76402.1; -; Genomic_DNA.
DR EMBL; BT002004; AAN72015.1; -; mRNA.
DR EMBL; BT006267; AAP13375.1; -; mRNA.
DR RefSeq; NP_188696.1; NM_112952.4.
DR AlphaFoldDB; O48915; -.
DR BioGRID; 6939; 2.
DR IntAct; O48915; 1.
DR STRING; 3702.AT3G20600.1; -.
DR PaxDb; O48915; -.
DR PRIDE; O48915; -.
DR ProteomicsDB; 251185; -.
DR DNASU; 821607; -.
DR EnsemblPlants; AT3G20600.1; AT3G20600.1; AT3G20600.
DR GeneID; 821607; -.
DR Gramene; AT3G20600.1; AT3G20600.1; AT3G20600.
DR KEGG; ath:AT3G20600; -.
DR Araport; AT3G20600; -.
DR TAIR; locus:2085725; AT3G20600.
DR eggNOG; ENOG502RZ5H; Eukaryota.
DR HOGENOM; CLU_107779_0_0_1; -.
DR InParanoid; O48915; -.
DR OMA; KSMAPRM; -.
DR OrthoDB; 1507382at2759; -.
DR PhylomeDB; O48915; -.
DR PRO; PR:O48915; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; O48915; baseline and differential.
DR Genevisible; O48915; AT.
DR GO; GO:0046658; C:anchored component of plasma membrane; IDA:UniProtKB.
DR GO; GO:0016020; C:membrane; ISS:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; IBA:GO_Central.
DR GO; GO:0042742; P:defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0050832; P:defense response to fungus; IMP:TAIR.
DR GO; GO:0098542; P:defense response to other organism; IMP:TAIR.
DR GO; GO:0009626; P:plant-type hypersensitive response; IMP:TAIR.
DR GO; GO:0010942; P:positive regulation of cell death; IMP:UniProtKB.
DR InterPro; IPR044839; NDR1-like.
DR PANTHER; PTHR31415; PTHR31415; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; GPI-anchor; Hypersensitive response; Immunity;
KW Innate immunity; Lipoprotein; Membrane; Plant defense; Proteoglycan;
KW Reference proteome; Signal.
FT SIGNAL 1..38
FT /evidence="ECO:0000255"
FT CHAIN 39..195
FT /note="Protein NDR1"
FT /id="PRO_0000403652"
FT PROPEP 196..219
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000403653"
FT LIPID 195
FT /note="GPI-anchor amidated aspartate"
FT /evidence="ECO:0000255"
FT CARBOHYD 62
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 88
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 93
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 98
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 107
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 132
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 142
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT MUTAGEN 1
FT /note="M->A: No effect on the interaction with RIN4."
FT /evidence="ECO:0000269|PubMed:17012600"
FT MUTAGEN 2
FT /note="N->A: No effect on the interaction with RIN4."
FT /evidence="ECO:0000269|PubMed:17012600"
FT MUTAGEN 3
FT /note="N->A: No effect on the interaction with RIN4."
FT /evidence="ECO:0000269|PubMed:17012600"
FT MUTAGEN 4
FT /note="Q->A: Loss of interaction with RIN4."
FT /evidence="ECO:0000269|PubMed:17012600"
FT MUTAGEN 5
FT /note="N->A: Loss of interaction with RIN4."
FT /evidence="ECO:0000269|PubMed:17012600"
FT MUTAGEN 6
FT /note="E->A: No effect on the interaction with RIN4."
FT /evidence="ECO:0000269|PubMed:17012600"
FT MUTAGEN 7
FT /note="D->A: No effect on the interaction with RIN4."
FT /evidence="ECO:0000269|PubMed:17012600"
FT MUTAGEN 8
FT /note="T->A: No effect on the interaction with RIN4."
FT /evidence="ECO:0000269|PubMed:17012600"
SQ SEQUENCE 219 AA; 24674 MW; DD53EF984F89D0DD CRC64;
MNNQNEDTEG GRNCCTCCLS FIFTAGLTSL FLWLSLRADK PKCSIQNFFI PALGKDPNSR
DNTTLNFMVR CDNPNKDKGI YYDDVHLNFS TINTTKINSS ALVLVGNYTV PKFYQGHKKK
AKKWGQVKPL NNQTVLRAVL PNGSAVFRLD LKTQVRFKIV FWKTKRYGVE VGADVEVNGD
GVKAQKKGIK MKKSDSSFPL RSSFPISVLM NLLVFFAIR
