NDRG4_MOUSE
ID NDRG4_MOUSE Reviewed; 352 AA.
AC Q8BTG7; Q6ZPW3; Q923D7;
DT 11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Protein NDRG4;
DE AltName: Full=N-myc downstream-regulated gene 4 protein;
DE AltName: Full=Protein Ndr4;
GN Name=Ndrg4; Synonyms=Kiaa1180, Ndr4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Heart, and Medulla oblongata;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Brain;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain cortex;
RX PubMed=17114649; DOI=10.1074/mcp.m600046-mcp200;
RA Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,
RA Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B.,
RA Panse C., Schlapbach R., Mansuy I.M.;
RT "Qualitative and quantitative analyses of protein phosphorylation in naive
RT and stimulated mouse synaptosomal preparations.";
RL Mol. Cell. Proteomics 6:283-293(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323, PHOSPHORYLATION [LARGE
RP SCALE ANALYSIS] AT SER-293 (ISOFORM 2), AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=21636852; DOI=10.1074/jbc.m111.256446;
RA Yamamoto H., Kokame K., Okuda T., Nakajo Y., Yanamoto H., Miyata T.;
RT "NDRG4 protein-deficient mice exhibit spatial learning deficits and
RT vulnerabilities to cerebral ischemia.";
RL J. Biol. Chem. 286:26158-26165(2011).
CC -!- FUNCTION: Contributes to the maintenance of intracerebral BDNF levels
CC within the normal range, which is necessary for the preservation of
CC spatial learning and the resistance to neuronal cell death caused by
CC ischemic stress. May enhance growth factor-induced ERK1 and ERK2
CC phosphorylation. May attenuate NGF-promoted ELK1 phosphorylation in a
CC microtubule-dependent manner. {ECO:0000269|PubMed:21636852}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8BTG7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BTG7-2; Sequence=VSP_022959;
CC Name=3;
CC IsoId=Q8BTG7-3; Sequence=VSP_022958;
CC -!- TISSUE SPECIFICITY: Predominantly expressed in the brain (at protein
CC level). Detected in neurons of various parts of brain, including the
CC olfactory bulb, olfactory tuberculum, cerebral cortex, striatum,
CC hippocampus, dentate gyrus, thalamus, hypothalamus, mesencephalon,
CC cerebellum, pons and medulla oblongata. {ECO:0000269|PubMed:21636852}.
CC -!- DISRUPTION PHENOTYPE: Mutant mice have impaired spatial learning and
CC memory but normal motor function. {ECO:0000269|PubMed:21636852}.
CC -!- SIMILARITY: Belongs to the NDRG family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC98115.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK090374; BAC41189.1; -; mRNA.
DR EMBL; AK168873; BAE40691.1; -; mRNA.
DR EMBL; AK129305; BAC98115.1; ALT_INIT; mRNA.
DR EMBL; BC006595; AAH06595.1; -; mRNA.
DR CCDS; CCDS22565.1; -. [Q8BTG7-2]
DR CCDS; CCDS57634.1; -. [Q8BTG7-1]
DR CCDS; CCDS90441.1; -. [Q8BTG7-3]
DR RefSeq; NP_001181935.1; NM_001195006.1. [Q8BTG7-1]
DR RefSeq; NP_663577.1; NM_145602.3. [Q8BTG7-2]
DR RefSeq; XP_006530930.1; XM_006530867.1.
DR AlphaFoldDB; Q8BTG7; -.
DR SMR; Q8BTG7; -.
DR BioGRID; 231544; 1.
DR STRING; 10090.ENSMUSP00000079495; -.
DR ESTHER; mouse-ndr4; Ndr_family.
DR MEROPS; S33.986; -.
DR iPTMnet; Q8BTG7; -.
DR PhosphoSitePlus; Q8BTG7; -.
DR MaxQB; Q8BTG7; -.
DR PaxDb; Q8BTG7; -.
DR PeptideAtlas; Q8BTG7; -.
DR PRIDE; Q8BTG7; -.
DR ProteomicsDB; 287463; -. [Q8BTG7-1]
DR ProteomicsDB; 287464; -. [Q8BTG7-2]
DR ProteomicsDB; 287465; -. [Q8BTG7-3]
DR Antibodypedia; 2919; 303 antibodies from 33 providers.
DR DNASU; 234593; -.
DR Ensembl; ENSMUST00000041318; ENSMUSP00000036226; ENSMUSG00000036564. [Q8BTG7-3]
DR Ensembl; ENSMUST00000073139; ENSMUSP00000072883; ENSMUSG00000036564. [Q8BTG7-1]
DR Ensembl; ENSMUST00000080666; ENSMUSP00000079495; ENSMUSG00000036564. [Q8BTG7-2]
DR GeneID; 234593; -.
DR KEGG; mmu:234593; -.
DR UCSC; uc009mys.2; mouse. [Q8BTG7-3]
DR UCSC; uc009myt.2; mouse. [Q8BTG7-2]
DR UCSC; uc009myu.2; mouse. [Q8BTG7-1]
DR CTD; 65009; -.
DR MGI; MGI:2384590; Ndrg4.
DR VEuPathDB; HostDB:ENSMUSG00000036564; -.
DR eggNOG; KOG2931; Eukaryota.
DR GeneTree; ENSGT00950000182872; -.
DR HOGENOM; CLU_035361_1_0_1; -.
DR InParanoid; Q8BTG7; -.
DR OMA; CMADSGG; -.
DR OrthoDB; 854690at2759; -.
DR PhylomeDB; Q8BTG7; -.
DR TreeFam; TF313168; -.
DR BioGRID-ORCS; 234593; 0 hits in 74 CRISPR screens.
DR ChiTaRS; Ndrg4; mouse.
DR PRO; PR:Q8BTG7; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q8BTG7; protein.
DR Bgee; ENSMUSG00000036564; Expressed in olfactory tubercle and 273 other tissues.
DR ExpressionAtlas; Q8BTG7; baseline and differential.
DR Genevisible; Q8BTG7; MM.
DR GO; GO:0016323; C:basolateral plasma membrane; ISO:MGI.
DR GO; GO:0031253; C:cell projection membrane; IDA:BHF-UCL.
DR GO; GO:0005737; C:cytoplasm; IDA:BHF-UCL.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISO:MGI.
DR GO; GO:0005739; C:mitochondrion; ISO:MGI.
DR GO; GO:0007420; P:brain development; IBA:GO_Central.
DR GO; GO:0060973; P:cell migration involved in heart development; IMP:BHF-UCL.
DR GO; GO:0010642; P:negative regulation of platelet-derived growth factor receptor signaling pathway; ISO:MGI.
DR GO; GO:0014912; P:negative regulation of smooth muscle cell migration; ISO:MGI.
DR GO; GO:0048662; P:negative regulation of smooth muscle cell proliferation; ISO:MGI.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; ISO:MGI.
DR GO; GO:0010976; P:positive regulation of neuron projection development; ISO:MGI.
DR GO; GO:2001135; P:regulation of endocytic recycling; IMP:BHF-UCL.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR GO; GO:0048278; P:vesicle docking; IMP:BHF-UCL.
DR GO; GO:0008542; P:visual learning; IMP:BHF-UCL.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR004142; NDRG.
DR InterPro; IPR030695; NDRG4.
DR PANTHER; PTHR11034; PTHR11034; 1.
DR PANTHER; PTHR11034:SF21; PTHR11034:SF21; 1.
DR Pfam; PF03096; Ndr; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..352
FT /note="Protein NDRG4"
FT /id="PRO_0000159580"
FT REGION 301..352
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 308..352
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 298
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Z2L9"
FT MOD_RES 317
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Z2L9"
FT MOD_RES 323
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..7
FT /note="MPECWDG -> MKVLGHRLQLLTGLLLHDVTMAGLQELRFPEEKPLLRGQDA
FT TEMDNPDAFLSIVDTDWK (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14621295"
FT /id="VSP_022958"
FT VAR_SEQ 289..302
FT /note="IAHLKDRRLSGGAV -> M (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_022959"
FT MOD_RES Q8BTG7-2:293
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 352 AA; 38509 MW; 3ED388148DC852CD CRC64;
MPECWDGEHD IETPYGLLHV VIRGSPKGNR PAILTYHDVG LNHKLCFNTF FNFEDMQEIT
KHFVVCHVDA PGQQVGASQF PQGYQFPSME QLAAMLPSVV QHFGFKYVIG IGVGAGAYVL
AKFALIFPDL VEGLVLMNID PNGKGWIDWA ATKLSGLTST LPDTVLSHLF SQEELVNNTE
LVQSYRQQIS NVVNQANLQL FWNMYNSRRD LDINRPGTVP NAKTLRCPVM LVVGDNAPAE
EGVVECNSKL DPTTTTFLKM ADSGGLPQVT QPGKLTEAFK YFLQGMGYIA HLKDRRLSGG
AVPSASMTRL ARSRTASLTS ASSVDGSRPQ PCAHSDSSEG MGQVNHTMEV SC
