NDRG4_RAT
ID NDRG4_RAT Reviewed; 352 AA.
AC Q9Z2L9; Q6XQ62; Q6XQ63; Q6XQ64; Q6XQ65; Q78ZK9; Q7TST8;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Protein NDRG4;
DE AltName: Full=Brain development-related molecule 1;
GN Name=Ndrg4; Synonyms=Bdm1, Ndr4;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC STRAIN=Long Evans; TISSUE=Brain;
RX PubMed=10320792; DOI=10.1016/s0169-328x(99)00084-4;
RA Yamauchi Y., Hongo S., Nishinaka N., Ohashi T., Takahashi R., Takeda M.;
RT "Molecular cloning and characterization of a novel developmentally
RT regulated gene, Bdm1, showing predominant expression in postnatal rat
RT brain.";
RL Brain Res. Mol. Brain Res. 68:149-158(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1; 2; 3; 4; 5 AND 6),
RP DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RC STRAIN=Long Evans;
RX PubMed=14693380; DOI=10.1016/j.gene.2003.09.029;
RA Maeda A., Hongo S., Miyazaki A.;
RT "Genomic organization, expression, and comparative analysis of noncoding
RT region of the rat Ndrg4 gene.";
RL Gene 324:149-158(2004).
RN [3]
RP FUNCTION.
RX PubMed=16408304; DOI=10.1002/jcb.20763;
RA Hongo S., Watanabe T., Takahashi K., Miyazaki A.;
RT "Ndrg4 enhances NGF-induced ERK activation uncoupled with Elk-1
RT activation.";
RL J. Cell. Biochem. 98:185-193(2006).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-298 AND SER-317, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Contributes to the maintenance of intracerebral BDNF levels
CC within the normal range, which is necessary for the preservation of
CC spatial learning and the resistance to neuronal cell death caused by
CC ischemic stress (By similarity). May enhance growth factor-induced ERK1
CC and ERK2 phosphorylation, including that induced by NGF. May attenuate
CC NGF-promoted ELK1 phosphorylation in a microtubule-dependent manner.
CC {ECO:0000250, ECO:0000269|PubMed:16408304}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=6;
CC Name=1; Synonyms=A1;
CC IsoId=Q9Z2L9-1; Sequence=Displayed;
CC Name=2; Synonyms=B1;
CC IsoId=Q9Z2L9-2; Sequence=VSP_022960;
CC Name=3; Synonyms=C1;
CC IsoId=Q9Z2L9-3; Sequence=VSP_022961;
CC Name=4; Synonyms=A2;
CC IsoId=Q9Z2L9-4; Sequence=VSP_022962;
CC Name=5; Synonyms=B2;
CC IsoId=Q9Z2L9-5; Sequence=VSP_022960, VSP_022962;
CC Name=6; Synonyms=C2;
CC IsoId=Q9Z2L9-6; Sequence=VSP_022961, VSP_022962;
CC -!- TISSUE SPECIFICITY: Expressed in the brain and heart, weakly in the
CC kidney; most prominently in postnatal brain where it is expressed
CC widely in the olfactory bulb, cerebral cortex, hippocampus, cerebellum,
CC thalamus, and medulla oblongata. {ECO:0000269|PubMed:10320792,
CC ECO:0000269|PubMed:14693380}.
CC -!- DEVELOPMENTAL STAGE: Isoform 1, isoform 2 and isoform 3 are detected in
CC maturing and mature brain, whereas isoform 4, isoform 5 and isoform 6
CC are expressed in embryonic and early postnatal brain. All isoforms are
CC expressed in heart. {ECO:0000269|PubMed:14693380}.
CC -!- SIMILARITY: Belongs to the NDRG family. {ECO:0000305}.
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DR EMBL; AF045564; AAD02415.1; -; mRNA.
DR EMBL; AY217029; AAO65543.1; -; mRNA.
DR EMBL; AY217030; AAO65544.1; -; mRNA.
DR EMBL; AY217031; AAO65545.1; -; mRNA.
DR EMBL; AY217032; AAO65546.1; -; mRNA.
DR EMBL; AY217033; AAO65547.1; -; mRNA.
DR EMBL; AF524894; AAP46191.1; -; Genomic_DNA.
DR EMBL; AF524894; AAP46192.1; -; Genomic_DNA.
DR EMBL; AY255791; AAQ17047.1; -; mRNA.
DR AlphaFoldDB; Q9Z2L9; -.
DR SMR; Q9Z2L9; -.
DR STRING; 10116.ENSRNOP00000044520; -.
DR ESTHER; ratno-ndr4; Ndr_family.
DR MEROPS; S33.986; -.
DR iPTMnet; Q9Z2L9; -.
DR PhosphoSitePlus; Q9Z2L9; -.
DR SwissPalm; Q9Z2L9; -.
DR PaxDb; Q9Z2L9; -.
DR PRIDE; Q9Z2L9; -.
DR UCSC; RGD:621593; rat. [Q9Z2L9-1]
DR RGD; 621593; Ndrg4.
DR eggNOG; KOG2931; Eukaryota.
DR InParanoid; Q9Z2L9; -.
DR PhylomeDB; Q9Z2L9; -.
DR PRO; PR:Q9Z2L9; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0016323; C:basolateral plasma membrane; ISO:RGD.
DR GO; GO:0031253; C:cell projection membrane; ISO:RGD.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:RGD.
DR GO; GO:0005739; C:mitochondrion; IDA:RGD.
DR GO; GO:0007420; P:brain development; IEP:RGD.
DR GO; GO:0060973; P:cell migration involved in heart development; ISO:RGD.
DR GO; GO:0010642; P:negative regulation of platelet-derived growth factor receptor signaling pathway; ISO:RGD.
DR GO; GO:0014912; P:negative regulation of smooth muscle cell migration; ISO:RGD.
DR GO; GO:0048662; P:negative regulation of smooth muscle cell proliferation; ISO:RGD.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IDA:BHF-UCL.
DR GO; GO:0010976; P:positive regulation of neuron projection development; IMP:RGD.
DR GO; GO:2001135; P:regulation of endocytic recycling; ISO:RGD.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR GO; GO:0048278; P:vesicle docking; ISO:RGD.
DR GO; GO:0008542; P:visual learning; ISO:RGD.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR004142; NDRG.
DR InterPro; IPR030695; NDRG4.
DR PANTHER; PTHR11034; PTHR11034; 1.
DR PANTHER; PTHR11034:SF21; PTHR11034:SF21; 1.
DR Pfam; PF03096; Ndr; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..352
FT /note="Protein NDRG4"
FT /id="PRO_0000159581"
FT REGION 301..352
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 308..352
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 298
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 317
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 323
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8BTG7"
FT VAR_SEQ 1..7
FT /note="MPECWDG -> MAGLQELRFPEEKPLLRGQDATELDNPDAFLSVVDTDWK
FT (in isoform 2 and isoform 5)"
FT /evidence="ECO:0000303|PubMed:14693380"
FT /id="VSP_022960"
FT VAR_SEQ 1..7
FT /note="MPECWDG -> MKVLGHRLQLPTGLLLHDVTMAGLQELRFPEEKPLLRGQDA
FT TELDNPDAFLSVVDTDWK (in isoform 3 and isoform 6)"
FT /evidence="ECO:0000303|PubMed:14693380"
FT /id="VSP_022961"
FT VAR_SEQ 289..302
FT /note="IAHLKDRRLSGGAV -> M (in isoform 4, isoform 5 and
FT isoform 6)"
FT /evidence="ECO:0000303|PubMed:14693380"
FT /id="VSP_022962"
SQ SEQUENCE 352 AA; 38487 MW; EF35D773B9D255D9 CRC64;
MPECWDGEHD IETPYGLLHV VIRGSPKGNR PAILTYHDVG LNHKLCFNTL FNLEDMQEIT
KHFVVCHVDA PGQQVGASQF PQGYQFPSME QLATMLPNVV QHFGFKYVIG IGVGAGAYVL
AKFALIFPDL VEGLVLMNID PNGKGWIDWA ATKLSGLTST LPDTVLSHLF SQEELVNNTE
LVQSYRQQIS SVVNQANLQL FWNMYNSRRD LDINRPGTVP NAKTLRCPVM LVVGDNAPAE
DGVVECNSKL DPTTTTFLKM ADSGGLPQVT QPGKLTEAFK YFLQGMGYIA HLKDRRLSGG
AVPSASMTRL ARSRTASLTS ASSVDGSRPQ PCTHSDSSEG MGQVNHTMEV SC
