NDS5B_ARATH
ID NDS5B_ARATH Reviewed; 83 AA.
AC Q9LZI6; Q8L9F7;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B;
GN OrderedLocusNames=At3g62790; ORFNames=F26K9_220;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Complex I is composed of at least 49 different subunits. This
CC is a component of the iron-sulfur (IP) fragment of the enzyme.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}. Mitochondrion inner
CC membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC Mitochondrion intermembrane space {ECO:0000305}.
CC -!- DOMAIN: Contains two C-X9-C motifs that are predicted to form a helix-
CC coil-helix structure, permitting the formation of intramolecular
CC disulfide bonds. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I NDUFS5 subunit family.
CC {ECO:0000305}.
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DR EMBL; AL162651; CAB83129.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE80392.1; -; Genomic_DNA.
DR EMBL; AY062842; AAL32920.1; -; mRNA.
DR EMBL; AY114576; AAM47895.1; -; mRNA.
DR EMBL; AY088460; AAM65996.1; -; mRNA.
DR PIR; T48068; T48068.
DR RefSeq; NP_191838.1; NM_116144.4.
DR PDB; 7A23; EM; 3.70 A; j=1-83.
DR PDB; 7A24; EM; 3.80 A; j=1-83.
DR PDB; 7AQQ; EM; 3.06 A; e=1-83.
DR PDB; 7AR8; EM; 3.53 A; e=1-83.
DR PDB; 7ARB; EM; 3.41 A; e=1-83.
DR PDBsum; 7A23; -.
DR PDBsum; 7A24; -.
DR PDBsum; 7AQQ; -.
DR PDBsum; 7AR8; -.
DR PDBsum; 7ARB; -.
DR AlphaFoldDB; Q9LZI6; -.
DR SMR; Q9LZI6; -.
DR BioGRID; 10768; 5.
DR IntAct; Q9LZI6; 5.
DR STRING; 3702.AT3G62790.1; -.
DR TCDB; 3.D.1.6.3; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR PaxDb; Q9LZI6; -.
DR PRIDE; Q9LZI6; -.
DR ProteomicsDB; 251186; -.
DR EnsemblPlants; AT3G62790.1; AT3G62790.1; AT3G62790.
DR GeneID; 825454; -.
DR Gramene; AT3G62790.1; AT3G62790.1; AT3G62790.
DR KEGG; ath:AT3G62790; -.
DR Araport; AT3G62790; -.
DR TAIR; locus:2081600; AT3G62790.
DR eggNOG; ENOG502S4B1; Eukaryota.
DR HOGENOM; CLU_162182_2_0_1; -.
DR InParanoid; Q9LZI6; -.
DR OMA; SRCFAYW; -.
DR OrthoDB; 1549192at2759; -.
DR PhylomeDB; Q9LZI6; -.
DR PRO; PR:Q9LZI6; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LZI6; baseline and differential.
DR Genevisible; Q9LZI6; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IEA:UniProtKB-SubCell.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; HDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; IBA:GO_Central.
DR InterPro; IPR019342; NADH_UbQ_OxRdtase_FeS-su5.
DR PANTHER; PTHR15224; PTHR15224; 1.
DR Pfam; PF10200; Ndufs5; 1.
DR PROSITE; PS51808; CHCH; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Electron transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Respiratory chain;
KW Transport.
FT CHAIN 1..83
FT /note="NADH dehydrogenase [ubiquinone] iron-sulfur protein
FT 5-B"
FT /id="PRO_0000410793"
FT DOMAIN 11..52
FT /note="CHCH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT REGION 62..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 14..24
FT /note="Cx9C motif 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 34..44
FT /note="Cx9C motif 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 14..44
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 24..34
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT CONFLICT 78
FT /note="G -> E (in Ref. 4; AAM65996)"
FT /evidence="ECO:0000305"
FT STRAND 9..11
FT /evidence="ECO:0007829|PDB:7AQQ"
FT HELIX 15..25
FT /evidence="ECO:0007829|PDB:7AQQ"
FT HELIX 35..46
FT /evidence="ECO:0007829|PDB:7AQQ"
FT HELIX 48..65
FT /evidence="ECO:0007829|PDB:7AQQ"
SQ SEQUENCE 83 AA; 9893 MW; 8E0502A0353871DD CRC64;
MASGWGITGN KGRCYDFWMD FSECMSHCRE PKDCTLLRED YLECLHHSKE FQRRNRIYKE
EQRKLRAASR KGEETGDGTH THH
