NDT1_ARATH
ID NDT1_ARATH Reviewed; 312 AA.
AC O22261; Q94JT4;
DT 09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Nicotinamide adenine dinucleotide transporter 1, chloroplastic;
DE Short=AtNDT1;
DE AltName: Full=NAD(+) transporter 1;
GN Name=NDT1; Synonyms=NADT1; OrderedLocusNames=At2g47490; ORFNames=T30B22.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=cv. Wassilewskija;
RX PubMed=16055441; DOI=10.1074/jbc.m506045200;
RA Bedhomme M., Hoffmann M., McCarthy E.A., Gambonnet B., Moran R.G.,
RA Rebeille F., Ravanel S.;
RT "Folate metabolism in plants: an Arabidopsis homolog of the mammalian
RT mitochondrial folate transporter mediates folate import into
RT chloroplasts.";
RL J. Biol. Chem. 280:34823-34831(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBSTRATE SPECIFICITY, ACTIVITY
RP REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=19745225; DOI=10.1074/jbc.m109.041830;
RA Palmieri F., Rieder B., Ventrella A., Blanco E., Do P.T., Nunes-Nesi A.,
RA Trauth A.U., Fiermonte G., Tjaden J., Agrimi G., Kirchberger S.,
RA Paradies E., Fernie A.R., Neuhaus H.E.;
RT "Molecular identification and functional characterization of Arabidopsis
RT thaliana mitochondrial and chloroplastic NAD+ carrier proteins.";
RL J. Biol. Chem. 284:31249-31259(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Mediates the NAD(+) import into chloroplast. Favors the
CC NAD(+)(in)/ADP or AMP(out) antiport exchange, but is also able to
CC catalyze a low unidirectional transport (uniport) of NAD(+). Transports
CC NAD(+), nicotinic acid adenine dinucleotide, nicotinamide
CC mononucleotide, nicotinic acid mononucleotide, FAD, FMN, TTP, TDP, TMP,
CC UTP, UDP, UMP, CTP, CDP, CMP, GTP, GDP, GMP, 3'-AMP, ATP, ADP, AND AMP,
CC has low transport activity with cAMP, pyrophosphate, NADH and alpha-
CC NAD(+), and has no activity with NADP(+), NADPH, nicotinamide,
CC nicotinic acid, adenosine, thiamine mono- or diphosphate, inorganic
CC phosphate, CoA, folate, NaCl, malate, malonate, citrate, fumarate,
CC aspartate, glutamate, S-adenosylmethionine, lysine, arginine, and
CC ornithine. {ECO:0000269|PubMed:16055441, ECO:0000269|PubMed:19745225}.
CC -!- ACTIVITY REGULATION: Inhibited by pyridoxal 5'-phosphate,
CC bathophenanthroline, tannic acid, mersalyl, mercuric chloride, p-
CC hydroxymercuribenzoate, p-hydroxymercuribenzoate sulfonate, bromocresol
CC purple and N-ethylmaleimide. {ECO:0000269|PubMed:19745225}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.24 mM for the NAD(+)/NAD(+) exchange
CC {ECO:0000269|PubMed:19745225};
CC Vmax=1.41 mmol/min/g enzyme for the NAD(+)/NAD(+) exchange
CC {ECO:0000269|PubMed:19745225};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Highly expressed in young leaf mesophyll cells,
CC root tips and at the branches of adventitious roots. Low expression in
CC all flower tissues and not detected in siliques and seeds.
CC {ECO:0000269|PubMed:19745225}.
CC -!- MISCELLANEOUS: Appears to be a chloroplast envelope-located membrane
CC protein lacking an N-terminal-located transit peptide.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; AJ871011; CAI38582.1; -; mRNA.
DR EMBL; FM211595; CAR70090.1; -; mRNA.
DR EMBL; AC002535; AAC62861.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC10850.1; -; Genomic_DNA.
DR EMBL; AF372957; AAK50096.1; -; mRNA.
DR EMBL; AY074833; AAL69531.1; -; mRNA.
DR EMBL; AK316807; BAH19521.1; -; mRNA.
DR EMBL; AY084889; AAM61452.1; -; mRNA.
DR PIR; T00435; T00435.
DR RefSeq; NP_566102.1; NM_130317.3.
DR AlphaFoldDB; O22261; -.
DR SMR; O22261; -.
DR STRING; 3702.AT2G47490.1; -.
DR TCDB; 2.A.29.10.11; the mitochondrial carrier (mc) family.
DR iPTMnet; O22261; -.
DR PaxDb; O22261; -.
DR PRIDE; O22261; -.
DR ProteomicsDB; 251106; -.
DR EnsemblPlants; AT2G47490.1; AT2G47490.1; AT2G47490.
DR GeneID; 819362; -.
DR Gramene; AT2G47490.1; AT2G47490.1; AT2G47490.
DR KEGG; ath:AT2G47490; -.
DR Araport; AT2G47490; -.
DR TAIR; locus:2062002; AT2G47490.
DR eggNOG; KOG0764; Eukaryota.
DR HOGENOM; CLU_015166_6_4_1; -.
DR InParanoid; O22261; -.
DR OMA; HVHEGWR; -.
DR OrthoDB; 1080385at2759; -.
DR PhylomeDB; O22261; -.
DR PRO; PR:O22261; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22261; baseline and differential.
DR Genevisible; O22261; AT.
DR GO; GO:0031969; C:chloroplast membrane; IDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0051724; F:NAD transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0035352; P:NAD transmembrane transport; IBA:GO_Central.
DR GO; GO:0043132; P:NAD transport; IDA:TAIR.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR002067; Mit_carrier.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR InterPro; IPR044712; SLC25A32-like.
DR PANTHER; PTHR45683; PTHR45683; 1.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00926; MITOCARRIER.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 1: Evidence at protein level;
KW Antiport; Chloroplast; Membrane; Plastid; Reference proteome; Repeat;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..312
FT /note="Nicotinamide adenine dinucleotide transporter 1,
FT chloroplastic"
FT /id="PRO_0000420695"
FT TRANSMEM 17..37
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..232
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 271..293
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 11..103
FT /note="Solcar 1"
FT REPEAT 111..199
FT /note="Solcar 2"
FT REPEAT 211..299
FT /note="Solcar 3"
SQ SEQUENCE 312 AA; 33883 MW; 95D5FF1039D3F634 CRC64;
MSANSHPPNS KNVLCNAAAG AAAGVVAATF VCPLDVIKTR FQVHGLPKLG DANIKGSLIV
GSLEQIFKRE GMRGLYRGLS PTVMALLSNW AIYFTMYDQL KSFLCSNDHK LSVGANVLAA
SGAGAATTIA TNPLWVVKTR LQTQGMRVGI VPYKSTFSAL RRIAYEEGIR GLYSGLVPAL
AGISHVAIQF PTYEMIKVYL AKKGDKSVDN LNARDVAVAS SIAKIFASTL TYPHEVVRAR
LQEQGHHSEK RYSGVRDCIK KVFEKDGFPG FYRGCATNLL RTTPAAVITF TSFEMVHRFL
VTHIPSEQSS IL
