NDUA2_BOVIN
ID NDUA2_BOVIN Reviewed; 99 AA.
AC Q02370; Q148D8; Q2VYC4;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2;
DE AltName: Full=Complex I-B8;
DE Short=CI-B8;
DE AltName: Full=NADH-ubiquinone oxidoreductase B8 subunit;
GN Name=NDUFA2;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 40-45; 47-61 AND 63-68.
RC TISSUE=Heart;
RX PubMed=1518044; DOI=10.1016/0022-2836(92)91052-q;
RA Walker J.E., Arizmendi J.M., Dupuis A., Fearnley I.M., Finel M., Medd S.M.,
RA Pilkington S.J., Runswick M.J., Skehel J.M.;
RT "Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine
RT heart mitochondria. Application of a novel strategy for sequencing proteins
RT using the polymerase chain reaction.";
RL J. Mol. Biol. 226:1051-1072(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Conrad M.L., Mawer M.A., Davis S.K., Koop B.F.;
RT "Genomic sequencing of the T cell receptor alpha locus.";
RL Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Fetal cerebellum;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP PARTIAL PROTEIN SEQUENCE, SUBUNIT, IDENTIFICATION IN COMPLEX I, AND
RP SUBCELLULAR LOCATION.
RX PubMed=10852722; DOI=10.1021/bi000335t;
RA Sazanov L.A., Peak-Chew S.Y., Fearnley I.M., Walker J.E.;
RT "Resolution of the membrane domain of bovine complex I into subcomplexes:
RT implications for the structural organization of the enzyme.";
RL Biochemistry 39:7229-7235(2000).
RN [5]
RP SUBUNIT, IDENTIFICATION IN COMPLEX I, AND SUBCELLULAR LOCATION.
RX PubMed=18721790; DOI=10.1016/j.ab.2008.07.029;
RA Lemma-Gray P., Valusova E., Carroll C.A., Weintraub S.T., Musatov A.,
RA Robinson N.C.;
RT "Subunit analysis of bovine heart complex I by reversed-phase high-
RT performance liquid chromatography, electrospray ionization-tandem mass
RT spectrometry, and matrix-assisted laser desorption/ionization-time-of-
RT flight mass spectrometry.";
RL Anal. Biochem. 382:116-121(2008).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone.
CC {ECO:0000250|UniProtKB:O43678}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC {ECO:0000269|PubMed:10852722, ECO:0000269|PubMed:18721790}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000305|PubMed:10852722, ECO:0000305|PubMed:18721790}; Peripheral
CC membrane protein {ECO:0000305}; Matrix side {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the complex I NDUFA2 subunit family.
CC {ECO:0000305}.
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DR EMBL; X63219; CAA44904.1; -; mRNA.
DR EMBL; AY227782; ABC02172.1; -; Genomic_DNA.
DR EMBL; BC118428; AAI18429.1; -; mRNA.
DR PIR; S28249; S28249.
DR RefSeq; NP_787009.1; NM_175815.2.
DR PDB; 5LC5; EM; 4.35 A; S=17-96.
DR PDB; 5LDW; EM; 4.27 A; S=1-99.
DR PDB; 5LDX; EM; 5.60 A; S=1-99.
DR PDB; 5O31; EM; 4.13 A; S=2-99.
DR PDB; 7QSD; EM; 3.10 A; S=1-99.
DR PDBsum; 5LC5; -.
DR PDBsum; 5LDW; -.
DR PDBsum; 5LDX; -.
DR PDBsum; 5O31; -.
DR PDBsum; 7QSD; -.
DR AlphaFoldDB; Q02370; -.
DR SMR; Q02370; -.
DR CORUM; Q02370; -.
DR DIP; DIP-38834N; -.
DR IntAct; Q02370; 18.
DR STRING; 9913.ENSBTAP00000020018; -.
DR TCDB; 3.D.1.6.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR PaxDb; Q02370; -.
DR PRIDE; Q02370; -.
DR Ensembl; ENSBTAT00000020018; ENSBTAP00000020018; ENSBTAG00000015041.
DR GeneID; 327698; -.
DR KEGG; bta:327698; -.
DR CTD; 4695; -.
DR VEuPathDB; HostDB:ENSBTAG00000015041; -.
DR eggNOG; KOG3446; Eukaryota.
DR GeneTree; ENSGT00390000006178; -.
DR HOGENOM; CLU_110897_0_0_1; -.
DR InParanoid; Q02370; -.
DR OMA; INSHYVA; -.
DR OrthoDB; 1633416at2759; -.
DR TreeFam; TF300229; -.
DR Reactome; R-BTA-611105; Respiratory electron transport.
DR Reactome; R-BTA-6799198; Complex I biogenesis.
DR Proteomes; UP000009136; Chromosome 7.
DR Bgee; ENSBTAG00000015041; Expressed in oocyte and 108 other tissues.
DR GO; GO:0031966; C:mitochondrial membrane; ISS:AgBase.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IDA:UniProtKB.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR InterPro; IPR016464; NADH_Ub_cplx-1_asu_su-2.
DR InterPro; IPR007741; Ribosome/NADH_DH.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR PANTHER; PTHR12878; PTHR12878; 1.
DR Pfam; PF05047; L51_S25_CI-B8; 1.
DR PIRSF; PIRSF005822; NDUA2; 1.
DR SMART; SM00916; L51_S25_CI-B8; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Direct protein sequencing; Disulfide bond;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Reference proteome; Respiratory chain; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:O43678"
FT CHAIN 2..99
FT /note="NADH dehydrogenase [ubiquinone] 1 alpha subcomplex
FT subunit 2"
FT /id="PRO_0000118788"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:O43678"
FT MOD_RES 64
FT /note="N6-acetyllysine; alternate"
FT /evidence="ECO:0000250|UniProtKB:Q9CQ75"
FT MOD_RES 64
FT /note="N6-succinyllysine; alternate"
FT /evidence="ECO:0000250|UniProtKB:Q9CQ75"
FT MOD_RES 75
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q9CQ75"
FT DISULFID 24..58
FT /note="Redox-active"
FT /evidence="ECO:0000250"
FT CONFLICT 44
FT /note="L -> V (in Ref. 2; ABC02172)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 99 AA; 11080 MW; B844420E64652EC2 CRC64;
MAAAAAIRGV RGKLGLREIR IHLCQRSPGS QGVRDFIEKR YVELKKANPD LPILIRECSD
VQPKLWARYA FGQEKNVSLN NFSADQVTRA LENVLSSKA
