NDUA3_MOUSE
ID NDUA3_MOUSE Reviewed; 84 AA.
AC Q9CQ91; Q0VBA7;
DT 31-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3;
DE AltName: Full=Complex I-B9;
DE Short=CI-B9;
DE AltName: Full=NADH-ubiquinone oxidoreductase B9 subunit;
GN Name=Ndufa3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Heart, and Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 48-58 AND 59-82, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone.
CC {ECO:0000250|UniProtKB:O95167}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:O95167}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:O95167}; Single-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the complex I NDUFA3 subunit family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK019099; BAB31545.1; -; mRNA.
DR EMBL; AK003133; BAB22593.1; -; mRNA.
DR EMBL; BC120712; AAI20713.1; -; mRNA.
DR EMBL; BC120714; AAI20715.1; -; mRNA.
DR CCDS; CCDS39728.1; -.
DR RefSeq; NP_079624.1; NM_025348.2.
DR PDB; 6G2J; EM; 3.30 A; b=1-84.
DR PDB; 6G72; EM; 3.90 A; b=1-84.
DR PDB; 6ZR2; EM; 3.10 A; b=1-84.
DR PDB; 6ZTQ; EM; 3.00 A; b=1-84.
DR PDB; 7AK5; EM; 3.17 A; b=2-84.
DR PDB; 7AK6; EM; 3.82 A; b=1-84.
DR PDB; 7B93; EM; 3.04 A; b=1-84.
DR PDB; 7PSA; EM; 3.40 A; b=1-84.
DR PDBsum; 6G2J; -.
DR PDBsum; 6G72; -.
DR PDBsum; 6ZR2; -.
DR PDBsum; 6ZTQ; -.
DR PDBsum; 7AK5; -.
DR PDBsum; 7AK6; -.
DR PDBsum; 7B93; -.
DR PDBsum; 7PSA; -.
DR AlphaFoldDB; Q9CQ91; -.
DR SMR; Q9CQ91; -.
DR BioGRID; 211208; 53.
DR ComplexPortal; CPX-266; Mitochondrial respiratory chain complex I.
DR CORUM; Q9CQ91; -.
DR IntAct; Q9CQ91; 1.
DR STRING; 10090.ENSMUSP00000075953; -.
DR PhosphoSitePlus; Q9CQ91; -.
DR EPD; Q9CQ91; -.
DR jPOST; Q9CQ91; -.
DR MaxQB; Q9CQ91; -.
DR PaxDb; Q9CQ91; -.
DR PeptideAtlas; Q9CQ91; -.
DR PRIDE; Q9CQ91; -.
DR ProteomicsDB; 252939; -.
DR Antibodypedia; 32779; 141 antibodies from 21 providers.
DR DNASU; 66091; -.
DR Ensembl; ENSMUST00000076657; ENSMUSP00000075953; ENSMUSG00000035674.
DR GeneID; 66091; -.
DR KEGG; mmu:66091; -.
DR UCSC; uc009eve.1; mouse.
DR CTD; 4696; -.
DR MGI; MGI:1913341; Ndufa3.
DR VEuPathDB; HostDB:ENSMUSG00000035674; -.
DR eggNOG; ENOG502S4RS; Eukaryota.
DR GeneTree; ENSGT00390000004322; -.
DR HOGENOM; CLU_171491_0_0_1; -.
DR InParanoid; Q9CQ91; -.
DR OMA; PDIPAHP; -.
DR OrthoDB; 1577370at2759; -.
DR PhylomeDB; Q9CQ91; -.
DR TreeFam; TF333021; -.
DR Reactome; R-MMU-611105; Respiratory electron transport.
DR Reactome; R-MMU-6799198; Complex I biogenesis.
DR BioGRID-ORCS; 66091; 21 hits in 72 CRISPR screens.
DR ChiTaRS; Ndufa3; mouse.
DR PRO; PR:Q9CQ91; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q9CQ91; protein.
DR Bgee; ENSMUSG00000035674; Expressed in cardiac muscle of left ventricle and 253 other tissues.
DR ExpressionAtlas; Q9CQ91; baseline and differential.
DR Genevisible; Q9CQ91; MM.
DR GO; GO:0005743; C:mitochondrial inner membrane; HDA:MGI.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0009060; P:aerobic respiration; IC:ComplexPortal.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR GO; GO:0042776; P:proton motive force-driven mitochondrial ATP synthesis; IC:ComplexPortal.
DR InterPro; IPR026626; NDUFA3.
DR PANTHER; PTHR15221; PTHR15221; 1.
DR Pfam; PF14987; NADHdh_A3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Direct protein sequencing; Electron transport;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Respiratory chain; Transmembrane; Transmembrane helix; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q02371"
FT CHAIN 2..84
FT /note="NADH dehydrogenase [ubiquinone] 1 alpha subcomplex
FT subunit 3"
FT /id="PRO_0000118794"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 59..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q02371"
FT HELIX 6..14
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 17..35
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 38..40
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 41..48
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 64..66
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 68..73
FT /evidence="ECO:0007829|PDB:6ZR2"
FT HELIX 78..80
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 81..83
FT /evidence="ECO:0007829|PDB:6ZTQ"
SQ SEQUENCE 84 AA; 9331 MW; B05E59B217D95A92 CRC64;
MAGRISAFLK NAWAKEPVLV VSFSVWGLAI IMPMISPYTK YASMINKATP YNYPVPVRDD
GNMPDVPSHP QDPLGPSLDW LKNL
