ID   NDUA3_NEUCR             Reviewed;          82 AA.
AC   P42117; Q7SHY3;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=NADH-ubiquinone oxidoreductase 9.5 kDa subunit;
DE   AltName: Full=Complex I-9.5kD;
DE            Short=CI-9.5;
DE   AltName: Full=Ubiquinone-binding protein;
GN   Name=nuo9.5; ORFNames=NCU00670;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 2-7.
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=1445879; DOI=10.1021/bi00161a021;
RA   Heinrich H., Azevedo J.E., Werner S.;
RT   "Characterization of the 9.5-kDa ubiquinone-binding protein of
RT   NADH:ubiquinone oxidoreductase (complex I) from Neurospora crassa.";
RL   Biochemistry 31:11420-11424(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
RN   [3]
RP   CHARACTERIZATION.
RX   PubMed=1445878; DOI=10.1021/bi00161a020;
RA   Heinrich H., Werner S.;
RT   "Identification of the ubiquinone-binding site of NADH:ubiquinone
RT   oxidoreductase (complex I) from Neurospora crassa.";
RL   Biochemistry 31:11413-11419(1992).
CC   -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC       chain NADH dehydrogenase (Complex I), that is believed not to be
CC       involved in catalysis. Complex I functions in the transfer of electrons
CC       from NADH to the respiratory chain. The immediate electron acceptor for
CC       the enzyme is believed to be ubiquinone. This subunit binds ubiquinone.
CC   -!- SUBUNIT: Complex I is composed of about 40 different subunits.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Single-pass
CC       membrane protein.
CC   -!- SIMILARITY: Belongs to the complex I NDUFA3 subunit family.
CC       {ECO:0000305}.
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DR   EMBL; S49807; AAB24322.1; -; mRNA.
DR   EMBL; CM002236; EAA36574.3; -; Genomic_DNA.
DR   PIR; A44210; A44210.
DR   RefSeq; XP_965810.3; XM_960717.3.
DR   AlphaFoldDB; P42117; -.
DR   SMR; P42117; -.
DR   STRING; 5141.EFNCRP00000000869; -.
DR   EnsemblFungi; EAA36574; EAA36574; NCU00670.
DR   GeneID; 3881935; -.
DR   KEGG; ncr:NCU00670; -.
DR   VEuPathDB; FungiDB:NCU00670; -.
DR   HOGENOM; CLU_2050261_0_0_1; -.
DR   InParanoid; P42117; -.
DR   Proteomes; UP000001805; Chromosome 1, Linkage Group I.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   InterPro; IPR039961; Nuo9.5.
DR   PANTHER; PTHR38488; PTHR38488; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Electron transport; Membrane; Mitochondrion;
KW   Mitochondrion inner membrane; Reference proteome; Respiratory chain;
KW   Transmembrane; Transmembrane helix; Transport.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:1445879"
FT   CHAIN           2..82
FT                   /note="NADH-ubiquinone oxidoreductase 9.5 kDa subunit"
FT                   /id="PRO_0000118795"
FT   TRANSMEM        25..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   82 AA;  9262 MW;  F7EC76B3246C4E77 CRC64;
     MSGTIPHFWA QPFRYIRWSA REKPAYFYSC VIAGLGPVFL TVVPPVRKYF GDVNPAPIPV
     TYPIPTGPRK QLTGYDDDTE EA