ID   NDUA4_DANRE             Reviewed;          82 AA.
AC   Q6PBH5;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Cytochrome c oxidase subunit NDUFA4;
GN   Name=ndufa4; ORFNames=zgc:73405;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the cytochrome c oxidase, the last enzyme in the
CC       mitochondrial electron transport chain which drives oxidative
CC       phosphorylation. The respiratory chain contains 3 multisubunit
CC       complexes succinate dehydrogenase (complex II, CII), ubiquinol-
CC       cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III,
CC       CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to
CC       transfer electrons derived from NADH and succinate to molecular oxygen,
CC       creating an electrochemical gradient over the inner membrane that
CC       drives transmembrane transport and the ATP synthase. Cytochrome c
CC       oxidase is the component of the respiratory chain that catalyzes the
CC       reduction of oxygen to water. Electrons originating from reduced
CC       cytochrome c in the intermembrane space (IMS) are transferred via the
CC       dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1
CC       to the active site in subunit 1, a binuclear center (BNC) formed by
CC       heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2
CC       water molecules unsing 4 electrons from cytochrome c in the IMS and 4
CC       protons from the mitochondrial matrix. NDUFA4 is required for complex
CC       IV maintenance. {ECO:0000250|UniProtKB:O00483}.
CC   -!- SUBUNIT: Component of the cytochrome c oxidase (complex IV, CIV), a
CC       multisubunit enzyme composed of 14 subunits. The complex is composed of
CC       a catalytic core of 3 subunits MT-CO1, MT-CO2 and MT-CO3, encoded in
CC       the mitochondrial DNA, and 11 supernumerary subunits COX4I, COX5A,
CC       COX5B, COX6A, COX6B, COX6C, COX7A, COX7B, COX7C, COX8 and NDUFA4, which
CC       are encoded in the nuclear genome. The complex exists as a monomer or a
CC       dimer and forms supercomplexes (SCs) in the inner mitochondrial
CC       membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and
CC       ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex
CC       III, CIII), resulting in different assemblies (supercomplex
CC       SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)) (By similarity).
CC       Interacts with RAB5IF (By similarity). {ECO:0000250|UniProtKB:O00483}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:O00483}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:O00483}.
CC   -!- SIMILARITY: Belongs to the complex IV NDUFA4 subunit family.
CC       {ECO:0000305}.
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DR   EMBL; BC059706; AAH59706.1; -; mRNA.
DR   RefSeq; NP_998190.1; NM_213025.1.
DR   AlphaFoldDB; Q6PBH5; -.
DR   SMR; Q6PBH5; -.
DR   STRING; 7955.ENSDARP00000055018; -.
DR   PaxDb; Q6PBH5; -.
DR   Ensembl; ENSDART00000055019; ENSDARP00000055018; ENSDARG00000056108.
DR   Ensembl; ENSDART00000182410; ENSDARP00000155008; ENSDARG00000116304.
DR   GeneID; 406298; -.
DR   KEGG; dre:406298; -.
DR   CTD; 406298; -.
DR   ZFIN; ZDB-GENE-040426-1962; ndufa4b.
DR   eggNOG; ENOG502S65P; Eukaryota.
DR   GeneTree; ENSGT00940000154268; -.
DR   HOGENOM; CLU_181002_0_0_1; -.
DR   InParanoid; Q6PBH5; -.
DR   OMA; QHKFYSP; -.
DR   OrthoDB; 1552648at2759; -.
DR   PhylomeDB; Q6PBH5; -.
DR   TreeFam; TF106383; -.
DR   Reactome; R-DRE-5628897; TP53 Regulates Metabolic Genes.
DR   Reactome; R-DRE-611105; Respiratory electron transport.
DR   Reactome; R-DRE-9707564; Cytoprotection by HMOX1.
DR   PRO; PR:Q6PBH5; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 12.
DR   Bgee; ENSDARG00000056108; Expressed in heart and 24 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005751; C:mitochondrial respiratory chain complex IV; ISS:UniProtKB.
DR   GO; GO:1904960; P:positive regulation of cytochrome-c oxidase activity; ISS:UniProtKB.
DR   InterPro; IPR010530; B12D.
DR   PANTHER; PTHR14256; PTHR14256; 1.
DR   Pfam; PF06522; B12D; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Reference proteome; Respiratory chain; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..82
FT                   /note="Cytochrome c oxidase subunit NDUFA4"
FT                   /id="PRO_0000245770"
FT   TOPO_DOM        1..14
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000250|UniProtKB:O00483"
FT   TRANSMEM        15..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:O00483"
FT   TOPO_DOM        38..82
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000250|UniProtKB:O00483"
SQ   SEQUENCE   82 AA;  9417 MW;  DE2F85D90254E0BD CRC64;
     MLATVMKQLK SHPALIPLFI FIGGGATMSM LYLGRLALKN PDCSWDRKNN PEPWNKLGPN
     DQYKLFSVNM DYSKLKKDRP DF