NDUA5_ARATH
ID NDUA5_ARATH Reviewed; 169 AA.
AC Q9FLX7;
DT 15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial;
DE Flags: Precursor;
GN OrderedLocusNames=At5g52840; ORFNames=MXC20.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:BAB10432.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAL79599.1}
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4] {ECO:0000305}
RP PROTEIN SEQUENCE OF 12-26.
RC TISSUE=Leaf, and Stem;
RX PubMed=11743114; DOI=10.1104/pp.010474;
RA Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P.;
RT "Proteomic approach to identify novel mitochondrial proteins in
RT Arabidopsis.";
RL Plant Physiol. 127:1694-1710(2001).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=14671022; DOI=10.1105/tpc.016055;
RA Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J.,
RA Millar A.H.;
RT "Experimental analysis of the Arabidopsis mitochondrial proteome highlights
RT signaling and regulatory components, provides assessment of targeting
RT prediction programs, and indicates plant-specific mitochondrial proteins.";
RL Plant Cell 16:241-256(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND CLEAVAGE OF TRANSIT PEPTIDE AFTER
RP LEU-11.
RX PubMed=25732537; DOI=10.1093/jxb/erv064;
RA Carrie C., Venne A.S., Zahedi R.P., Soll J.;
RT "Identification of cleavage sites and substrate proteins for two
RT mitochondrial intermediate peptidases in Arabidopsis thaliana.";
RL J. Exp. Bot. 66:2691-2708(2015).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Complex I is composed of at least 49 different subunits.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250,
CC ECO:0000305|PubMed:25732537}; Peripheral membrane protein
CC {ECO:0000250}; Matrix side {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I NDUFA5 subunit family.
CC {ECO:0000305}.
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DR EMBL; AB009055; BAB10432.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96267.1; -; Genomic_DNA.
DR EMBL; AY054251; AAL06910.1; -; mRNA.
DR EMBL; AY079117; AAL79599.1; -; mRNA.
DR RefSeq; NP_568778.1; NM_124662.4.
DR PDB; 7A23; EM; 3.70 A; R=1-169.
DR PDB; 7A24; EM; 3.80 A; R=1-169.
DR PDB; 7AQR; EM; 2.91 A; V=1-169.
DR PDB; 7AR7; EM; 3.72 A; V=12-151.
DR PDB; 7AR8; EM; 3.53 A; V=1-169.
DR PDB; 7ARB; EM; 3.41 A; V=1-169.
DR PDBsum; 7A23; -.
DR PDBsum; 7A24; -.
DR PDBsum; 7AQR; -.
DR PDBsum; 7AR7; -.
DR PDBsum; 7AR8; -.
DR PDBsum; 7ARB; -.
DR AlphaFoldDB; Q9FLX7; -.
DR SMR; Q9FLX7; -.
DR BioGRID; 20606; 1.
DR IntAct; Q9FLX7; 2.
DR STRING; 3702.AT5G52840.1; -.
DR TCDB; 3.D.1.6.3; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR iPTMnet; Q9FLX7; -.
DR SwissPalm; Q9FLX7; -.
DR PaxDb; Q9FLX7; -.
DR PRIDE; Q9FLX7; -.
DR ProteomicsDB; 251322; -.
DR EnsemblPlants; AT5G52840.1; AT5G52840.1; AT5G52840.
DR GeneID; 835361; -.
DR Gramene; AT5G52840.1; AT5G52840.1; AT5G52840.
DR KEGG; ath:AT5G52840; -.
DR Araport; AT5G52840; -.
DR TAIR; locus:2176917; AT5G52840.
DR eggNOG; KOG3365; Eukaryota.
DR HOGENOM; CLU_099943_1_0_1; -.
DR InParanoid; Q9FLX7; -.
DR OrthoDB; 1372253at2759; -.
DR PhylomeDB; Q9FLX7; -.
DR BioCyc; ARA:AT5G52840-MON; -.
DR BioCyc; MetaCyc:AT5G52840-MON; -.
DR PRO; PR:Q9FLX7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FLX7; baseline and differential.
DR Genevisible; Q9FLX7; AT.
DR GO; GO:0031966; C:mitochondrial membrane; IDA:TAIR.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; HDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0045271; C:respiratory chain complex I; IDA:TAIR.
DR GO; GO:0009853; P:photorespiration; TAS:TAIR.
DR GO; GO:0022904; P:respiratory electron transport chain; IBA:GO_Central.
DR InterPro; IPR006806; NDUFA5.
DR PANTHER; PTHR12653; PTHR12653; 1.
DR Pfam; PF04716; ETC_C1_NDUFA5; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Electron transport; Membrane;
KW Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Respiratory chain; Transit peptide; Transport.
FT TRANSIT 1..11
FT /note="Mitochondrion"
FT /evidence="ECO:0000269|PubMed:11743114,
FT ECO:0000269|PubMed:25732537"
FT CHAIN 12..169
FT /note="Probable NADH dehydrogenase [ubiquinone] 1 alpha
FT subcomplex subunit 5, mitochondrial"
FT /id="PRO_0000019995"
FT STRAND 19..22
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 30..43
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 44..46
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 52..70
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 74..81
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 86..106
FT /evidence="ECO:0007829|PDB:7AQR"
FT STRAND 116..119
FT /evidence="ECO:0007829|PDB:7AQR"
FT STRAND 128..130
FT /evidence="ECO:0007829|PDB:7AQR"
FT HELIX 140..150
FT /evidence="ECO:0007829|PDB:7AQR"
SQ SEQUENCE 169 AA; 19179 MW; 5C0FD0C062DFC31F CRC64;
MFLRAIGRPL LAKVKQTTGI VGLDVVPNAR AVLIDLYSKT LKEIQAVPED EGYRKAVESF
TRQRLNVCKE EEDWEMIEKR LGCGQVEELI EEARDELTLI GKMIEWDPWG VPDDYECEVI
ENDAPIPKHV PQHRPGPLPE QFYKTLEGLI AESKTEIPAA TPSDPQLKE
