NDUA5_BOVIN
ID NDUA5_BOVIN Reviewed; 116 AA.
AC P23935; Q32P63;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5;
DE AltName: Full=Complex I subunit B13;
DE AltName: Full=Complex I-13kD-B;
DE Short=CI-13kD-B;
DE AltName: Full=NADH-ubiquinone oxidoreductase 13 kDa-B subunit;
GN Name=NDUFA5;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND SUBCELLULAR
RP LOCATION.
RC TISSUE=Heart;
RX PubMed=1518044; DOI=10.1016/0022-2836(92)91052-q;
RA Walker J.E., Arizmendi J.M., Dupuis A., Fearnley I.M., Finel M., Medd S.M.,
RA Pilkington S.J., Runswick M.J., Skehel J.M.;
RT "Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine
RT heart mitochondria. Application of a novel strategy for sequencing proteins
RT using the polymerase chain reaction.";
RL J. Mol. Biol. 226:1051-1072(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PROTEIN SEQUENCE OF 2-116, ACETYLATION AT ALA-2, AND SUBCELLULAR LOCATION.
RC TISSUE=Heart;
RX PubMed=1907966; DOI=10.1093/oxfordjournals.jbchem.a123416;
RA Masui R., Wakabayashi S., Matsubara H., Hatefi Y.;
RT "The amino acid sequences of two 13 kDa polypeptides and partial amino acid
RT sequence of 30 kDa polypeptide of complex I from bovine heart mitochondria:
RT possible location of iron-sulfur clusters.";
RL J. Biochem. 109:534-543(1991).
RN [4]
RP PARTIAL PROTEIN SEQUENCE, SUBUNIT, IDENTIFICATION IN COMPLEX I, AND
RP SUBCELLULAR LOCATION.
RX PubMed=10852722; DOI=10.1021/bi000335t;
RA Sazanov L.A., Peak-Chew S.Y., Fearnley I.M., Walker J.E.;
RT "Resolution of the membrane domain of bovine complex I into subcomplexes:
RT implications for the structural organization of the enzyme.";
RL Biochemistry 39:7229-7235(2000).
RN [5]
RP SUBUNIT, IDENTIFICATION IN COMPLEX I, AND SUBCELLULAR LOCATION.
RX PubMed=18721790; DOI=10.1016/j.ab.2008.07.029;
RA Lemma-Gray P., Valusova E., Carroll C.A., Weintraub S.T., Musatov A.,
RA Robinson N.C.;
RT "Subunit analysis of bovine heart complex I by reversed-phase high-
RT performance liquid chromatography, electrospray ionization-tandem mass
RT spectrometry, and matrix-assisted laser desorption/ionization-time-of-
RT flight mass spectrometry.";
RL Anal. Biochem. 382:116-121(2008).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed not to be
CC involved in catalysis. Complex I functions in the transfer of electrons
CC from NADH to the respiratory chain. The immediate electron acceptor for
CC the enzyme is believed to be ubiquinone.
CC {ECO:0000250|UniProtKB:Q16718}.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC {ECO:0000269|PubMed:10852722, ECO:0000269|PubMed:18721790}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000305|PubMed:10852722, ECO:0000305|PubMed:1518044,
CC ECO:0000305|PubMed:18721790, ECO:0000305|PubMed:1907966}; Peripheral
CC membrane protein; Matrix side.
CC -!- SIMILARITY: Belongs to the complex I NDUFA5 subunit family.
CC {ECO:0000305}.
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DR EMBL; X63218; CAA44903.1; -; mRNA.
DR EMBL; BC108243; AAI08244.1; -; mRNA.
DR PIR; S28244; S28244.
DR RefSeq; NP_787023.1; NM_175829.2.
DR PDB; 5LC5; EM; 4.35 A; V=1-116.
DR PDB; 5LDW; EM; 4.27 A; V=2-116.
DR PDB; 5LDX; EM; 5.60 A; V=2-116.
DR PDB; 5O31; EM; 4.13 A; V=2-116.
DR PDB; 7QSD; EM; 3.10 A; V=1-116.
DR PDBsum; 5LC5; -.
DR PDBsum; 5LDW; -.
DR PDBsum; 5LDX; -.
DR PDBsum; 5O31; -.
DR PDBsum; 7QSD; -.
DR AlphaFoldDB; P23935; -.
DR SMR; P23935; -.
DR CORUM; P23935; -.
DR DIP; DIP-38832N; -.
DR IntAct; P23935; 4.
DR STRING; 9913.ENSBTAP00000012287; -.
DR TCDB; 3.D.1.6.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR iPTMnet; P23935; -.
DR PaxDb; P23935; -.
DR PRIDE; P23935; -.
DR Ensembl; ENSBTAT00000012287; ENSBTAP00000012287; ENSBTAG00000009334.
DR GeneID; 327714; -.
DR KEGG; bta:327714; -.
DR CTD; 4698; -.
DR VEuPathDB; HostDB:ENSBTAG00000009334; -.
DR VGNC; VGNC:31949; NDUFA5.
DR eggNOG; KOG3365; Eukaryota.
DR GeneTree; ENSGT00390000008099; -.
DR HOGENOM; CLU_099943_2_0_1; -.
DR InParanoid; P23935; -.
DR OMA; VNEHPHR; -.
DR OrthoDB; 1372253at2759; -.
DR TreeFam; TF313785; -.
DR Reactome; R-BTA-611105; Respiratory electron transport.
DR Reactome; R-BTA-6799198; Complex I biogenesis.
DR Reactome; R-BTA-9013408; RHOG GTPase cycle.
DR Proteomes; UP000009136; Chromosome 4.
DR Bgee; ENSBTAG00000009334; Expressed in tongue muscle and 104 other tissues.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IDA:UniProtKB.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR GO; GO:0022904; P:respiratory electron transport chain; IBA:GO_Central.
DR InterPro; IPR006806; NDUFA5.
DR PANTHER; PTHR12653; PTHR12653; 1.
DR Pfam; PF04716; ETC_C1_NDUFA5; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Direct protein sequencing; Electron transport;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Phosphoprotein;
KW Reference proteome; Respiratory chain; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:1907966"
FT CHAIN 2..116
FT /note="NADH dehydrogenase [ubiquinone] 1 alpha subcomplex
FT subunit 5"
FT /id="PRO_0000118631"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000269|PubMed:1907966"
FT MOD_RES 30
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q16718"
FT MOD_RES 46
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q9CPP6"
FT MOD_RES 60
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q16718"
FT MOD_RES 89
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q63362"
FT MOD_RES 98
FT /note="N6-acetyllysine; alternate"
FT /evidence="ECO:0000250|UniProtKB:Q9CPP6"
FT MOD_RES 98
FT /note="N6-succinyllysine; alternate"
FT /evidence="ECO:0000250|UniProtKB:Q9CPP6"
FT CONFLICT 108
FT /note="P -> W (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 112
FT /note="Missing (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 116 AA; 13316 MW; 8AD6550D2ECD7B0D CRC64;
MAGLLKKTTG LVGLAVCETP HERLKILYTK ILDVLGHIPK NAAYRKYTEQ ITNEKLSMVK
AEPDVKKLEE RLQGGQIEEV ILQAENELSL ARKMIQWKPW EPLVEEPPAS QWKWPI
