NDUA6_MOUSE
ID NDUA6_MOUSE Reviewed; 131 AA.
AC Q9CQZ5;
DT 27-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 {ECO:0000305};
DE AltName: Full=Complex I-B14;
DE Short=CI-B14;
DE AltName: Full=NADH-ubiquinone oxidoreductase B14 subunit;
GN Name=Ndufa6 {ECO:0000312|MGI:MGI:1914380};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, Embryonic liver, and Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 10-26; 45-60 AND 81-88, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane respiratory
CC chain NADH dehydrogenase (Complex I), that is believed to be not
CC involved in catalysis. Required for proper complex I assembly. Complex
CC I functions in the transfer of electrons from NADH to the respiratory
CC chain. The immediate electron acceptor for the enzyme is believed to be
CC ubiquinone. {ECO:0000250|UniProtKB:P56556}.
CC -!- SUBUNIT: Mammalian complex I is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:P56556}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P56556}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P56556}; Matrix side
CC {ECO:0000250|UniProtKB:P56556}.
CC -!- SIMILARITY: Belongs to the complex I LYR family. {ECO:0000305}.
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DR EMBL; AK002749; BAB22326.1; -; mRNA.
DR EMBL; AK010988; BAB27308.1; -; mRNA.
DR EMBL; AK012328; BAB28165.1; -; mRNA.
DR EMBL; BC048451; AAH48451.1; -; mRNA.
DR CCDS; CCDS27689.1; -.
DR RefSeq; NP_080263.1; NM_025987.3.
DR PDB; 6G2J; EM; 3.30 A; W=1-131.
DR PDB; 6G72; EM; 3.90 A; W=1-131.
DR PDB; 6ZR2; EM; 3.10 A; W=1-131.
DR PDB; 6ZTQ; EM; 3.00 A; W=1-131.
DR PDB; 7AK5; EM; 3.17 A; W=1-131.
DR PDB; 7AK6; EM; 3.82 A; W=1-131.
DR PDB; 7B93; EM; 3.04 A; W=1-131.
DR PDB; 7PSA; EM; 3.40 A; W=1-131.
DR PDBsum; 6G2J; -.
DR PDBsum; 6G72; -.
DR PDBsum; 6ZR2; -.
DR PDBsum; 6ZTQ; -.
DR PDBsum; 7AK5; -.
DR PDBsum; 7AK6; -.
DR PDBsum; 7B93; -.
DR PDBsum; 7PSA; -.
DR AlphaFoldDB; Q9CQZ5; -.
DR SMR; Q9CQZ5; -.
DR BioGRID; 211963; 60.
DR ComplexPortal; CPX-266; Mitochondrial respiratory chain complex I.
DR CORUM; Q9CQZ5; -.
DR IntAct; Q9CQZ5; 3.
DR STRING; 10090.ENSMUSP00000023085; -.
DR iPTMnet; Q9CQZ5; -.
DR PhosphoSitePlus; Q9CQZ5; -.
DR EPD; Q9CQZ5; -.
DR jPOST; Q9CQZ5; -.
DR MaxQB; Q9CQZ5; -.
DR PaxDb; Q9CQZ5; -.
DR PeptideAtlas; Q9CQZ5; -.
DR PRIDE; Q9CQZ5; -.
DR ProteomicsDB; 287467; -.
DR Antibodypedia; 45919; 119 antibodies from 22 providers.
DR DNASU; 67130; -.
DR Ensembl; ENSMUST00000023085; ENSMUSP00000023085; ENSMUSG00000022450.
DR GeneID; 67130; -.
DR KEGG; mmu:67130; -.
DR UCSC; uc007wzc.2; mouse.
DR CTD; 4700; -.
DR MGI; MGI:1914380; Ndufa6.
DR VEuPathDB; HostDB:ENSMUSG00000022450; -.
DR eggNOG; KOG3426; Eukaryota.
DR GeneTree; ENSGT00390000018898; -.
DR HOGENOM; CLU_111660_3_0_1; -.
DR InParanoid; Q9CQZ5; -.
DR OMA; MVINPTY; -.
DR OrthoDB; 1518948at2759; -.
DR PhylomeDB; Q9CQZ5; -.
DR TreeFam; TF105625; -.
DR Reactome; R-MMU-611105; Respiratory electron transport.
DR Reactome; R-MMU-6799198; Complex I biogenesis.
DR BioGRID-ORCS; 67130; 19 hits in 74 CRISPR screens.
DR ChiTaRS; Ndufa6; mouse.
DR PRO; PR:Q9CQZ5; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q9CQZ5; protein.
DR Bgee; ENSMUSG00000022450; Expressed in right kidney and 257 other tissues.
DR ExpressionAtlas; Q9CQZ5; baseline and differential.
DR Genevisible; Q9CQZ5; MM.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISO:MGI.
DR GO; GO:0031966; C:mitochondrial membrane; ISO:MGI.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0009060; P:aerobic respiration; IC:ComplexPortal.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR GO; GO:0042776; P:proton motive force-driven mitochondrial ATP synthesis; IC:ComplexPortal.
DR GO; GO:0006979; P:response to oxidative stress; ISO:MGI.
DR CDD; cd20266; Complex1_LYR_NDUFA6_LYRM6; 1.
DR InterPro; IPR008011; Complex1_LYR_dom.
DR InterPro; IPR045299; Complex1_LYR_NDUFA6_LYRM6.
DR InterPro; IPR016488; NADH_Ub_cplx-1_asu_su-6.
DR PANTHER; PTHR12964; PTHR12964; 1.
DR Pfam; PF05347; Complex1_LYR; 1.
DR PIRSF; PIRSF006643; NDUA6; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Electron transport; Membrane;
KW Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Respiratory chain; Transport.
FT CHAIN 1..131
FT /note="NADH dehydrogenase [ubiquinone] 1 alpha subcomplex
FT subunit 6"
FT /id="PRO_0000174303"
FT STRAND 26..28
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 29..52
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 59..71
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 72..75
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 79..97
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 98..100
FT /evidence="ECO:0007829|PDB:7AK5"
FT HELIX 103..107
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 108..111
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 112..114
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 121..126
FT /evidence="ECO:0007829|PDB:6ZTQ"
SQ SEQUENCE 131 AA; 15283 MW; 3D829A5BA1D42FC5 CRC64;
MAAAATGLRQ AAAAAASTSV KPIFSRDLNE AKRRVRELYR AWYREVPNTV HLMQLDITVK
QGRDKVREMF MKNAHVTDPR VVDLLVIKGK MELQETIKVW KQRTHVMRFF HETETPRPKD
FLSKFYMGHD P
